Abstract
Recombinant viruses based on the cDNA copy of the tobacco mosaic virus (TMV) genome carrying different versions of the conserved M2e epitope from influenza virus A cloned into the coat protein (CP) gene were obtained and partially characterized by our group previously; cysteines in the human consensus M2e sequence were changed to serine residues. This work intends to show some biological properties of these viruses following plant infections. Agroinfiltration experiments on Nicotiana benthamiana confirmed the efficient systemic expression of M2e peptides, and two point amino acid substitutions in recombinant CPs significantly influenced the symptoms and development of viral infections. Joint expression of RNA interference suppressor protein p19 from tomato bushy stunt virus (TBSV) did not affect the accumulation of CP-M2e-ser recombinant protein in non-inoculated leaves. RT-PCR analysis of RNA isolated from either infected leaves or purified TMV-M2e particles proved the genetic stability of TMV‑based viral vectors. Immunoelectron microscopy of crude plant extracts demonstrated that foreign epitopes are located on the surface of chimeric virions. The rod‑shaped geometry of plant-produced M2e epitopes is different from the icosahedral or helical filamentous arrangement of M2e antigens on the carrier virus-like particles (VLP) described earlier. Thereby, we created a simple and efficient system that employs agrobacteria and plant viral vectors in order to produce a candidate broad-spectrum flu vaccine.
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Claims 7
Claim · model-written summary
Agroinfiltration confirmed efficient systemic M2e expression in Nicotiana benthamiana.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Outcome
- efficient systemic expression of M2e peptidese0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Agroinfiltration experiments on Nicotiana benthamiana confirmed the efficient systemic expression of M2e peptides”
Claim · model-written summary
Two coat protein substitutions significantly influenced infection symptoms and development.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Treatment
- two point amino acid substitutions in recombinant CPse0
- Outcome
- symptoms and development of viral infectionse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two point amino acid substitutions in recombinant CPs significantly influenced the symptoms and development of viral infections.”
t0“Plants”
e1“Agroinfiltration experiments on Nicotiana benthamiana confirmed the efficient systemic expression of M2e peptides”
Claim · model-written summary
Joint p19 expression did not affect CP-M2e-ser accumulation in non-inoculated leaves.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Treatment
- Joint expression of RNA interference suppressor protein p19e0
- Gene
- p19e0
- Outcome
- accumulation of CP-M2e-ser recombinant proteine0
- Direction
- did not affecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Joint expression of RNA interference suppressor protein p19 from tomato bushy stunt virus (TBSV) did not affect the accumulation of CP-M2e-ser recombinant protein in non-inoculated leaves.”
t0“Plants”
e1“Agroinfiltration experiments on Nicotiana benthamiana confirmed the efficient systemic expression of M2e peptides”
Claim · model-written summary
RT-PCR established genetic stability of TMV-based viral vectors.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Outcome
- genetic stability of TMV‑based viral vectorse0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RT-PCR analysis of RNA isolated from either infected leaves or purified TMV-M2e particles proved the genetic stability of TMV‑based viral vectors.”
t0“Plants”
e1“Agroinfiltration experiments on Nicotiana benthamiana confirmed the efficient systemic expression of M2e peptides”
Claim · model-written summary
Immunoelectron microscopy showed epitopes on chimeric virion surfaces.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Outcome
- foreign epitopes are located on the surface of chimeric virionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Immunoelectron microscopy of crude plant extracts demonstrated that foreign epitopes are located on the surface of chimeric virions.”
t0“Plants”
e1“Agroinfiltration experiments on Nicotiana benthamiana confirmed the efficient systemic expression of M2e peptides”
Claim · model-written summary
Plant-produced M2e epitope geometry differed from previously described carrier particles.
Verbatim
e0“The rod‑shaped geometry of plant-produced M2e epitopes is different from the icosahedral or helical filamentous arrangement of M2e antigens on the carrier virus-like particles (VLP) described earlier.”
Claim · model-written summary
The authors report a simple system using agrobacteria and plant viral vectors to produce a candidate flu vaccine.
Not reportedComparatorValue
Verbatim
e0“we created a simple and efficient system that employs agrobacteria and plant viral vectors in order to produce a candidate broad-spectrum flu vaccine.”
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