Abstract
In a search for Eucalyptus grandis Hill ex Maiden MADS-box genes involved in floral initiation and development, we isolated two cDNAs (EgrMADS 3 and EgrMADS 4), which are functional orthologues of the Arabidopsis thaliana (L.) Heynh. floral activator gene SOC1. These two E. grandis genes are equally and most-actively expressed in vegetative tissues such as apical shoots and young leaves. The two genes are less actively, but differentially expressed in roots and unopened flowers. Ectopic expression of EgrMADS 3 or EgrMADS 4 in the late-flowering A. thaliana Ler soc1 (agl20) derivative complemented the phenotype of this mutant, and some of these transgenic lines flowered significantly earlier than the wild-type Ler ecotype. Overexpression of EgrMADS 3 or EgrMADS 4 in A. thaliana ecotype Columbia accelerated flowering time under short-day conditions. However, under the same conditions the flowering time of A. thaliana ecotype C24 was altered by the ectopic expression of EgrMADS 3, but not that of EgrMADS 4.The nucleotide sequence data reported will appear in the EMBL and GenBank Nucleotide Databases under the accession numbers AY263807 (EgrMADS 3) and AY263808 (EgrMADS 4).
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Claims 8
Claim · model-written summary
EgrMADS 3 and EgrMADS 4 of Eucalyptus grandis are functional orthologues of the Arabidopsis SOC1 floral activator gene.
- Taxon
- E. grandis
Eucalyptus grandise1 - Gene
- EgrMADS 3 and EgrMADS 4e0
- Outcome
- functional orthologues of the Arabidopsis thaliana (L.) Heynh. floral activator gene SOC1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we isolated two cDNAs (EgrMADS 3 and EgrMADS 4), which are functional orthologues of the Arabidopsis thaliana (L.) Heynh. floral activator gene SOC1”
e1“These two E. grandis genes”
Claim · model-written summary
EgrMADS 3 and EgrMADS 4 are equally and most actively expressed in apical shoots and young leaves of Eucalyptus grandis.
- Taxon
- E. grandisEucalyptus grandise1
- Organ / stage
- vegetative tissues such as apical shoots and young leavese1
- Outcome
- most-actively expressede1
- Qualifier
- equallye1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“These two E. grandis genes are equally and most-actively expressed in vegetative tissues such as apical shoots and young leaves”
Claim · model-written summary
EgrMADS 3 and EgrMADS 4 are less actively but differentially expressed in roots and unopened flowers of Eucalyptus grandis.
- Taxon
- E. grandisEucalyptus grandise1
- Organ / stage
- roots and unopened flowerse2
- Outcome
- differentially expressede2
- Qualifier
- less activelye2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“These two E. grandis genes are equally and most-actively expressed in vegetative tissues such as apical shoots and young leaves”
e2“The two genes are less actively, but differentially expressed in roots and unopened flowers”
Claim · model-written summary
Ectopic expression of EgrMADS 3 or EgrMADS 4 complemented the phenotype of the Arabidopsis soc1 mutant.
- Taxon
- A. thaliana
Arabidopsis thalianae3 - Material
- Ler soc1 (agl20) derivativee3
- Treatment
- Ectopic expressione3
- Gene
- EgrMADS 3 or EgrMADS 4e3
- Outcome
- phenotype of this mutante3
- Direction
- complementede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“Ectopic expression of EgrMADS 3 or EgrMADS 4 in the late-flowering A. thaliana Ler soc1 (agl20) derivative complemented the phenotype of this mutant”
Claim · model-written summary
Some transgenic Arabidopsis lines with ectopic expression flowered significantly earlier than wild-type Ler.
- Taxon
- A. thalianaArabidopsis thalianae3
- Material
- transgenic linese4
- Treatment
- Ectopic expressione3
- Outcome
- flowerede4
- Direction
- earliere4
- Comparator
- wild-type Ler ecotypee4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“Ectopic expression of EgrMADS 3 or EgrMADS 4 in the late-flowering A. thaliana Ler soc1 (agl20) derivative complemented the phenotype of this mutant”
e4“some of these transgenic lines flowered significantly earlier than the wild-type Ler ecotype”
Claim · model-written summary
Overexpression of EgrMADS 3 or EgrMADS 4 accelerated flowering time of Arabidopsis ecotype Columbia under short-day conditions.
- Taxon
- A. thalianaArabidopsis thalianae5
- Material
- ecotype Columbiae5
- Treatment
- Overexpression of EgrMADS 3 or EgrMADS 4e5
- Gene
- EgrMADS 3 or EgrMADS 4e5
- Outcome
- flowering timee5
- Direction
- acceleratede5
- Setting
- short-day conditionse5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“Overexpression of EgrMADS 3 or EgrMADS 4 in A. thaliana ecotype Columbia accelerated flowering time under short-day conditions”
Claim · model-written summary
Ectopic expression of EgrMADS 3 altered the flowering time of Arabidopsis ecotype C24.
- Taxon
- A. thalianaArabidopsis thalianae6
- Material
- ecotype C24e6
- Treatment
- ectopic expression of EgrMADS 3e6
- Gene
- EgrMADS 3e6
- Outcome
- flowering timee6
- Direction
- alterede6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“However, under the same conditions the flowering time of A. thaliana ecotype C24 was altered by the ectopic expression of EgrMADS 3, but not that of EgrMADS 4”
Claim · model-written summary
Ectopic expression of EgrMADS 4 did not change the flowering time of Arabidopsis ecotype C24.
- Taxon
- A. thalianaArabidopsis thalianae6
- Material
- ecotype C24e6
- Treatment
- ectopic expressione6
- Gene
- EgrMADS 4e6
- Outcome
- flowering timee6
- Direction
- no_changeinferrede6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“However, under the same conditions the flowering time of A. thaliana ecotype C24 was altered by the ectopic expression of EgrMADS 3, but not that of EgrMADS 4”
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See all 19 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.