Abstract
In plants of Sinapis alba induced to flower by one long day, the MADS box gene, SaMADS A, is expressed initially in the central corpus (L3 cells) of the shoot apical meristem (SAM), about 1.5-2 days before initiation of the first floral meristem. We have combined a physiological approach by testing the effects of three putative floral signals on SaMADS A expression in the SAM of S. alba plants with a transgenic approach using Arabidopsis thaliana plants. A single application of a low dose of a cytokinin or a gibberellin to the apex of vegetative S. alba plants is capable of mimicking perfectly the initial effect of the long day on SaMADS A transcription. A treatment combining the two hormones causes the same activation but seems to enhance the level of SaMADS A expression. A sucrose application to the apex of vegetative plants is, on the contrary, unable to activate SaMADS A expression. None of these chemicals, alone or combined, is capable of causing the floral shift at the SAM. Since the constitutive expression of SaMADS A leads to precocious flowering in A. thaliana and antisense expression of a fragment of the A. thaliana homologue AGL20 leads to a delay in flowering time, these results are consistent with SaMADS A activation being an intermediate event in a cytokinin- and/or gibberellin-triggered signal transduction pathway that is involved in the regulation of floral transition in S. alba.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 9
Claim · model-written summary
SaMADS A was expressed initially in central corpus cells before floral-meristem initiation in Sinapis alba.
- Taxon
- Sinapis alba
Sinapis albat0 - Gene
- SaMADS Ae0
- Outcome
- expressed initially in the central corpuse0
- Value
- 1.5-2 dayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SaMADS A, is expressed initially in the central corpus (L3 cells) of the shoot apical meristem (SAM), about 1.5-2 days before initiation of the first floral meristem.”
t0“Sinapis alba”
Claim · model-written summary
Cytokinin application activated SaMADS A transcription similarly to a long day.
- Taxon
- S. albaSinapis albae0
- Treatment
- a cytokinine0
- Gene
- SaMADS Ae0
- Outcome
- SaMADS A transcriptione0
- Direction
- mimicking perfectlye0
- Comparator
- the long daye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A single application of a low dose of a cytokinin or a gibberellin to the apex of vegetative S. alba plants is capable of mimicking perfectly the initial effect of the long day on SaMADS A transcription.”
Claim · model-written summary
Gibberellin application activated SaMADS A transcription similarly to a long day.
- Taxon
- S. albaSinapis albae0
- Treatment
- a gibberelline0
- Gene
- SaMADS Ae0
- Outcome
- SaMADS A transcriptione0
- Direction
- mimicking perfectlye0
- Comparator
- the long daye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A single application of a low dose of a cytokinin or a gibberellin to the apex of vegetative S. alba plants is capable of mimicking perfectly the initial effect of the long day on SaMADS A transcription.”
Claim · model-written summary
Combined hormones activated SaMADS A and appeared to enhance expression.
- Taxon
- Sinapis albaSinapis albat0
- Treatment
- combining the two hormonese0
- Gene
- SaMADS Ae0
- Outcome
- level of SaMADS A expressione0
- Direction
- enhancee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A treatment combining the two hormones causes the same activation but seems to enhance the level of SaMADS A expression.”
t0“Sinapis alba”
Claim · model-written summary
Sucrose application did not activate SaMADS A expression.
- Taxon
- Sinapis albaSinapis albat0
- Treatment
- sucrose applicatione0
- Gene
- SaMADS Ae0
- Outcome
- SaMADS A expressione0
- Direction
- unable to activatee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A sucrose application to the apex of vegetative plants is, on the contrary, unable to activate SaMADS A expression.”
t0“Sinapis alba”
Claim · model-written summary
None of the chemicals, alone or combined, caused a floral shift.
- Taxon
- Sinapis albaSinapis albat0
- Treatment
- these chemicals, alone or combinede0
- Outcome
- floral shift at the SAMe0
- Direction
- Nonee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“None of these chemicals, alone or combined, is capable of causing the floral shift at the SAM.”
t0“Sinapis alba”
Claim · model-written summary
Constitutive SaMADS A expression led to precocious flowering in Arabidopsis thaliana.
- Taxon
- A. thaliana
Arabidopsis thalianae0 - Treatment
- constitutive expression of SaMADS Ae0
- Gene
- SaMADS Ae0
- Outcome
- precocious floweringe0
- Direction
- precociouse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“constitutive expression of SaMADS A leads to precocious flowering in A. thaliana”
Claim · model-written summary
Antisense expression of AGL20 delayed flowering in Arabidopsis thaliana.
- Taxon
- A. thalianaArabidopsis thalianae0
- Treatment
- antisense expression of a fragment of the A. thaliana homologue AGL20e0
- Gene
- AGL20e0
- Outcome
- flowering timee0
- Direction
- delaye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“antisense expression of a fragment of the A. thaliana homologue AGL20 leads to a delay in flowering time”
Claim · model-written summary
SaMADS A activation was interpreted as an intermediate in hormone-triggered floral-transition signaling.
- Taxon
- S. albaSinapis albae0
- Gene
- SaMADS Ae0
- Outcome
- regulation of floral transitione0
- Qualifier
- intermediate event in a cytokinin- and/or gibberellin-triggered signal transduction pathwaye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SaMADS A activation being an intermediate event in a cytokinin- and/or gibberellin-triggered signal transduction pathway that is involved in the regulation of floral transition in S. alba.”
The same questions in other papers
transgenic · phenology · Arabidopsis thaliana (thale cress)Positive and negative results
2 papers in the same direction as this paper · 3 in the opposite direction
- positive
- positive
- positive
2 papers more in this group
See all 6 papers →loss of function · phenology · Arabidopsis thaliana (thale cress)Positive and negative results
8 papers in the same direction as this paper · 6 in the opposite direction
- positive
- positive
- positive
11 papers more in this group
See all 15 papers →overexpression · phenology · Arabidopsis thaliana (thale cress)Positive and negative results
8 papers in the same direction as this paper · 10 in the opposite direction
- no effect
- no effect
- negative
15 papers more in this group
See all 19 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.