Paper · 2005 · HortScience

Cloning and Characterization of Five MADS Box Genes in Peach (Prunus persica)

Yong Xu; Fan Wu; Rong-Cai Ma

14
claims
27
verbatim quotes
0
citations
5
research areas
✓
Open access

Abstract

MADS box genes regulate most of the development processes in plants. Studying peach MADS box genes will provide insights into its flower and fruit development. Five MADS box cDNAs with complete coding regions were cloned in this study. PpMADS2 cDNA is 1116-bp long. RT-PCR analysis indicated that PpMADS2 is expressed in leaf, flower, fruit, and nutlet. PpMAD4 cDNA is 824-bp long, which is the homologue of Agamous. RT-PCR analysis indicated that PpMADS4 is expressed in the two inner parts of flower, fruit, and nutlet; and was absent in leaf and the two outer parts of the flower. This expression pattern is similar to that of Agamous gene in A. thaliana . PpMADS4 could promote the flowering process in A. thaliana tested by genetic transformation. PpMADS5 cDNA is 873-bp long, which is the homologue of SEP3. RT-PCR analysis indicated that PpMADS5 is expressed in the three inner parts of flower, fruit, and nutlet; and was absent in leaf and sepal, similar to the expression pattern of SEP3 gene in Arabidopsis . PpMAD6 cDNA is 1037-bp long, which is the homologue of FUL. RT-PCR analysis indicated that PpMADS6 is expressed in leaf, sepal, petal, carpel, and fruit; and was absent in stamen and nutlet. PpMAD7 cDNA is 1147-bp long, which is the homologue of SEP1. RT-PCR analysis indicated that PpMADS7 is expressed in the four parts of flower and fruit, and was absent in leaf, stamen, and nutlet. Furthermore, two SSRs were identified in the 5' UTR in the two MADS box genes, PpMADS2 and PpMADS7, respectively. The SSR in PpMAD2 was more polymorphic than that in PpMADS7 in the 39 Prunus accessions collected.

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Claims 14

Method / resourceobservedoriginal resultmeasured

Claim · model-written summary

Five peach MADS box cDNAs with complete coding regions were cloned.

Outcome
MADS box cDNAse0
Value
Fivee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Prunus persica
Prunus persica · TaxonAllagash Brewing · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e0

“Five MADS box cDNAs with complete coding regions were cloned in this study.”

✓ located verbatim in the source · abstract, characters 160–235 · show in text ↑
t0

“Peach (Prunus persica)”

✓ located verbatim in the source · title, characters 55–77 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

PpMADS2 cDNA is 1116 bp long.

Gene
PpMADS2e0
Outcome
PpMADS2 cDNAe0
Value
1116-bpe0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Prunus persica
Prunus persica · TaxonAllagash Brewing · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e0

“PpMADS2 cDNA is 1116-bp long.”

✓ located verbatim in the source · abstract, characters 236–265 · show in text ↑
t0

“Peach (Prunus persica)”

✓ located verbatim in the source · title, characters 55–77 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

PpMADS2 is expressed in leaf, flower, fruit and nutlet.

Gene
PpMADS2e0
Outcome
expressed in leaf, flower, fruit, and nutlete0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Prunus persica
Prunus persica · TaxonAllagash Brewing · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e0

“PpMADS2 is expressed in leaf, flower, fruit, and nutlet.”

✓ located verbatim in the source · abstract, characters 297–353 · show in text ↑
t0

“Peach (Prunus persica)”

✓ located verbatim in the source · title, characters 55–77 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

PpMADS4 cDNA is 824 bp long and homologous to Agamous.

Gene
PpMAD4e0
Outcome
homologue of Agamouse0
Value
824-bpe0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Prunus persica
Prunus persica · TaxonAllagash Brewing · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e0

“PpMAD4 cDNA is 824-bp long, which is the homologue of Agamous.”

✓ located verbatim in the source · abstract, characters 354–416 · show in text ↑
t0

“Peach (Prunus persica)”

✓ located verbatim in the source · title, characters 55–77 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

PpMADS4 is expressed in the inner flower parts, fruit and nutlet, but absent from leaf and outer flower parts.

Gene
PpMADS4e0
Outcome
expressed in the two inner parts of flower, fruit, and nutlet; and was absent in leaf and the two outer parts of the flowere0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Prunus persica
Prunus persica · TaxonAllagash Brewing · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e0

“PpMADS4 is expressed in the two inner parts of flower, fruit, and nutlet; and was absent in leaf and the two outer parts of the flower.”

✓ located verbatim in the source · abstract, characters 448–583 · show in text ↑
t0

“Peach (Prunus persica)”

✓ located verbatim in the source · title, characters 55–77 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

PpMADS4 promoted flowering in transformed Arabidopsis.

Taxon
A. thalianaArabidopsis thalianae0
Treatment
genetic transformatione0
Gene
PpMADS4e0
Outcome
flowering processe0
Direction
promotee0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Arabidopsis thaliana
Arabidopsis thaliana · TaxonOriginal uploader was Brona at en.wikipedia . User:Roepers at nl.wikipedia · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“PpMADS4 could promote the flowering process in A. thaliana tested by genetic transformation.”

✓ located verbatim in the source · abstract, characters 660–752 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

PpMADS5 cDNA is 873 bp long and homologous to SEP3.

Gene
PpMADS5e0
Outcome
homologue of SEP3e0
Value
873-bpe0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Prunus persica
Prunus persica · TaxonAllagash Brewing · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e0

“PpMADS5 cDNA is 873-bp long, which is the homologue of SEP3.”

✓ located verbatim in the source · abstract, characters 753–813 · show in text ↑
t0

“Peach (Prunus persica)”

✓ located verbatim in the source · title, characters 55–77 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

PpMADS5 is expressed in inner flower parts, fruit and nutlet, but absent from leaf and sepal.

Gene
PpMADS5e0
Outcome
expressed in the three inner parts of flower, fruit, and nutlet; and was absent in leaf and sepale0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Prunus persica
Prunus persica · TaxonAllagash Brewing · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e0

“PpMADS5 is expressed in the three inner parts of flower, fruit, and nutlet; and was absent in leaf and sepal”

✓ located verbatim in the source · abstract, characters 845–953 · show in text ↑
t0

“Peach (Prunus persica)”

✓ located verbatim in the source · title, characters 55–77 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

PpMADS6 cDNA is 1037 bp long and homologous to FUL.

Gene
PpMAD6e0
Outcome
homologue of FULe0
Value
1037-bpe0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Prunus persica
Prunus persica · TaxonAllagash Brewing · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e0

“PpMAD6 cDNA is 1037-bp long, which is the homologue of FUL.”

✓ located verbatim in the source · abstract, characters 1019–1078 · show in text ↑
t0

“Peach (Prunus persica)”

✓ located verbatim in the source · title, characters 55–77 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

PpMADS6 is expressed in leaf, sepal, petal, carpel and fruit, but absent in stamen and nutlet.

Gene
PpMADS6e0
Outcome
expressed in leaf, sepal, petal, carpel, and fruit; and was absent in stamen and nutlete0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Prunus persica
Prunus persica · TaxonAllagash Brewing · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e0

“PpMADS6 is expressed in leaf, sepal, petal, carpel, and fruit; and was absent in stamen and nutlet.”

✓ located verbatim in the source · abstract, characters 1110–1209 · show in text ↑
t0

“Peach (Prunus persica)”

✓ located verbatim in the source · title, characters 55–77 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

PpMADS7 cDNA is 1147 bp long and homologous to SEP1.

Gene
PpMAD7e0
Outcome
homologue of SEP1e0
Value
1147-bpe0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Prunus persica
Prunus persica · TaxonAllagash Brewing · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e0

“PpMAD7 cDNA is 1147-bp long, which is the homologue of SEP1.”

✓ located verbatim in the source · abstract, characters 1210–1270 · show in text ↑
t0

“Peach (Prunus persica)”

✓ located verbatim in the source · title, characters 55–77 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

PpMADS7 is expressed in four flower parts and fruit and was reported absent in leaf, stamen and nutlet.

Gene
PpMADS7e0
Outcome
expressed in the four parts of flower and fruit, and was absent in leaf, stamen, and nutlete0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Prunus persica
Prunus persica · TaxonAllagash Brewing · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e0

“PpMADS7 is expressed in the four parts of flower and fruit, and was absent in leaf, stamen, and nutlet.”

✓ located verbatim in the source · abstract, characters 1302–1405 · show in text ↑
t0

“Peach (Prunus persica)”

✓ located verbatim in the source · title, characters 55–77 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Two SSRs were identified in the 5′ UTR of PpMADS2 and PpMADS7.

Outcome
SSRs were identified in the 5' UTRe0
Value
twoe0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Prunus persica
Prunus persica · TaxonAllagash Brewing · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e0

“two SSRs were identified in the 5' UTR in the two MADS box genes, PpMADS2 and PpMADS7, respectively.”

✓ located verbatim in the source · abstract, characters 1419–1519 · show in text ↑
t0

“Peach (Prunus persica)”

✓ located verbatim in the source · title, characters 55–77 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The SSR in PpMAD2 was more polymorphic than that in PpMADS7 across 39 Prunus accessions.

Gene
PpMAD2e0
Outcome
polymorphice0
Direction
moree0
Value
39 Prunus accessionse0
Comparator
PpMADS7e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Prunus persica
Prunus persica · TaxonAllagash Brewing · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e0

“The SSR in PpMAD2 was more polymorphic than that in PpMADS7 in the 39 Prunus accessions collected.”

✓ located verbatim in the source · abstract, characters 1520–1618 · show in text ↑
t0

“Peach (Prunus persica)”

✓ located verbatim in the source · title, characters 55–77 · show in text ↑

transgenic · phenology · Arabidopsis thaliana (thale cress)Positive and negative results

6 papers: 3 positive, 3 negative or no effect

2 papers in the same direction as this paper · 3 in the opposite direction

  1. negative
  2. no effect
  3. no effect

2 papers more in this group

See all 6 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.