Abstract
FLOWERING LOCUS T (FT) plays a key role as a mobile floral induction signal that initiates the floral transition. Therefore, precise control of FT expression is critical for the reproductive success of flowering plants. Coexistence of bivalent histone H3 lysine 27 trimethylation (H3K27me3) and H3K4me3 marks at the FT locus and the role of H3K27me3 as a strong FT repression mechanism in Arabidopsis have been reported. However, the role of an active mark, H3K4me3, in FT regulation has not been addressed, nor have the components affecting this mark been identified. Mutations in Arabidopsis thaliana Jumonji4 (AtJmj4) and EARLY FLOWERING6 (ELF6), two Arabidopsis genes encoding Jumonji (Jmj) family proteins, caused FT-dependent, additive early flowering correlated with increased expression of FT mRNA and increased H3K4me3 levels within FT chromatin. Purified recombinant AtJmj4 protein possesses specific demethylase activity for mono-, di-, and trimethylated H3K4. Tagged AtJmj4 and ELF6 proteins associate directly with the FT transcription initiation region, a region where the H3K4me3 levels were increased most significantly in the mutants. Thus, our study demonstrates the roles of AtJmj4 and ELF6 as H3K4 demethylases directly repressing FT chromatin and preventing precocious flowering in Arabidopsis.
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Claims 7
Claim · model-written summary
AtJmj4 and ELF6 mutations caused additive FT dependent early flowering.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Treatment
- Mutations in Arabidopsis thaliana Jumonji4 (AtJmj4) and EARLY FLOWERING6 (ELF6)e0
- Gene
- AtJmj4e0
- Outcome
- early floweringe0
- Direction
- earlye0
- Qualifier
- FT-dependent, additivee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mutations in Arabidopsis thaliana Jumonji4 (AtJmj4) and EARLY FLOWERING6 (ELF6), two Arabidopsis genes encoding Jumonji (Jmj) family proteins, caused FT-dependent, additive early flowering correlated with increased expression of FT mRNA and increased H3K4me3 levels within FT chromatin.”
Claim · model-written summary
Mutations in AtJmj4 and ELF6 were associated with increased FT mRNA expression.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- Mutations in Arabidopsis thaliana Jumonji4 (AtJmj4) and EARLY FLOWERING6 (ELF6)e0
- Gene
- FTe0
- Outcome
- expression of FT mRNAe0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mutations in Arabidopsis thaliana Jumonji4 (AtJmj4) and EARLY FLOWERING6 (ELF6), two Arabidopsis genes encoding Jumonji (Jmj) family proteins, caused FT-dependent, additive early flowering correlated with increased expression of FT mRNA and increased H3K4me3 levels within FT chromatin.”
Claim · model-written summary
Mutations in AtJmj4 and ELF6 were associated with increased H3K4me3 within FT chromatin.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- Mutations in Arabidopsis thaliana Jumonji4 (AtJmj4) and EARLY FLOWERING6 (ELF6)e0
- Gene
- FTe0
- Outcome
- H3K4me3 levels within FT chromatine0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mutations in Arabidopsis thaliana Jumonji4 (AtJmj4) and EARLY FLOWERING6 (ELF6), two Arabidopsis genes encoding Jumonji (Jmj) family proteins, caused FT-dependent, additive early flowering correlated with increased expression of FT mRNA and increased H3K4me3 levels within FT chromatin.”
Claim · model-written summary
Purified AtJmj4 demethylated mono, di and trimethylated H3K4.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- AtJmj4 proteine0
- Outcome
- specific demethylase activity for mono-, di-, and trimethylated H3K4e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Purified recombinant AtJmj4 protein possesses specific demethylase activity for mono-, di-, and trimethylated H3K4.”
t0“Arabidopsis”
Claim · model-written summary
AtJmj4 and ELF6 proteins associated directly with the FT transcription initiation region.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- AtJmj4 and ELF6 proteinse0
- Outcome
- associate directly with the FT transcription initiation regione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Tagged AtJmj4 and ELF6 proteins associate directly with the FT transcription initiation region, a region where the H3K4me3 levels were increased most significantly in the mutants.”
t0“Arabidopsis”
Claim · model-written summary
The FT initiation region showed the greatest increase in H3K4me3 in mutants.
- Taxon
- ArabidopsisArabidopsist0
- Material
- mutantse0
- Gene
- FTe0
- Outcome
- H3K4me3 levelse0
- Direction
- increased most significantlye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Tagged AtJmj4 and ELF6 proteins associate directly with the FT transcription initiation region, a region where the H3K4me3 levels were increased most significantly in the mutants.”
t0“Arabidopsis”
Claim · model-written summary
The authors conclude that AtJmj4 and ELF6 directly repress FT chromatin and prevent premature flowering.
- Taxon
- ArabidopsisArabidopsise0
- Gene
- AtJmj4 and ELF6e0
- Outcome
- preventing precocious floweringe0
- Qualifier
- directly repressing FT chromatine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Thus, our study demonstrates the roles of AtJmj4 and ELF6 as H3K4 demethylases directly repressing FT chromatin and preventing precocious flowering in Arabidopsis.”
The same questions in other papers
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5 papers in the same direction as this paper · 9 in the opposite direction
- no effect
- negative
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11 papers more in this group
See all 15 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.