Abstract
Melon necrotic spot virus (MNSV) (genus Carmovirus, family Tombusviridae) is a single-stranded, positive-sense RNA virus that has become an experimental model for the analysis of cell-to-cell virus movement and translation of uncapped viral RNAs, whereas little is known about its replication. Analysis of the cytopathology after MNSV infection showed the specific presence of modified organelles that resemble mitochondria. Immunolocalization of the glycine decarboxylase complex (GDC) P protein in these organelles confirmed their mitochondrial origin. In situ hybridization and immunolocalization experiments showed the specific localization of positive-sense viral RNA, capsid protein (CP), and double-stranded (ds)RNA in these organelles meaning that replication of the virus takes place in association with them. The three-dimensional reconstructions of the altered mitochondria showed the presence of large, interconnected, internal dilations which appeared to be linked to the outside cytoplasmic environment through pores and/or complex structures, and with lipid bodies. Transient expression of MNSV p29 revealed that its specific target is mitochondria. Our data document the extensive reorganization of host mitochondria induced by MNSV, which provides a protected environment to viral replication, and show that the MNSV p29 protein is the primary determinant of this effect in the host.
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Claims 8
Claim · model-written summary
MNSV infection produced modified organelles that resemble mitochondria.
- Taxon
- Melon
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Cucumis melot0 - Interactor
- MNSV
Melon necrotic spot viruse1pathogen - Outcome
- modified organelles that resemble mitochondriae1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
t0“Melon necrotic spot virus”
e1“Analysis of the cytopathology after MNSV infection showed the specific presence of modified organelles that resemble mitochondria”
Claim · model-written summary
Immunolocalization of the GDC P protein confirmed the mitochondrial origin of the modified organelles.
- Taxon
- MelonCucumis melot0
- Gene
- glycine decarboxylase complex (GDC) P proteine2
- Outcome
- mitochondrial origine2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Melon necrotic spot virus”
e2“Immunolocalization of the glycine decarboxylase complex (GDC) P protein in these organelles confirmed their mitochondrial origin”
Claim · model-written summary
Positive-sense viral RNA, capsid protein and dsRNA were localised in the modified organelles.
- Taxon
- MelonCucumis melot0
- Interactor
- MNSVMelon necrotic spot viruse1pathogen
- Outcome
- specific localization of positive-sense viral RNA, capsid protein (CP), and double-stranded (ds)RNAe3
- Qualifier
- in these organellese3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Melon necrotic spot virus”
e1“Analysis of the cytopathology after MNSV infection showed the specific presence of modified organelles that resemble mitochondria”
e3“In situ hybridization and immunolocalization experiments showed the specific localization of positive-sense viral RNA, capsid protein (CP), and double-stranded (ds)RNA in these organelles”
Claim · model-written summary
Replication of the virus takes place in association with the altered mitochondria.
- Taxon
- MelonCucumis melot0
- Outcome
- replication of the viruse4
- Qualifier
- in association with theme4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Melon necrotic spot virus”
e4“meaning that replication of the virus takes place in association with them”
Claim · model-written summary
The altered mitochondria contain large, interconnected, internal dilations linked to the cytoplasm through pores or complex structures and with lipid bodies.
- Taxon
- MelonCucumis melot0
- Organ / stage
- altered mitochondriae5
- Outcome
- large, interconnected, internal dilationse5
- Qualifier
- linked to the outside cytoplasmic environment through pores and/or complex structures, and with lipid bodiese5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Melon necrotic spot virus”
e5“The three‐dimensional reconstructions of the altered mitochondria showed the presence of large, interconnected, internal dilations which appeared to be linked to the outside cytoplasmic environment through pores and/or complex structures, and with lipid bodies”
Claim · model-written summary
The MNSV p29 protein has mitochondria as its specific target.
- Taxon
- MelonCucumis melot0
- Gene
- MNSV p29e6
- Outcome
- specific target is mitochondriae6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Melon necrotic spot virus”
e6“Transient expression of MNSV p29 revealed that its specific target is mitochondria”
Claim · model-written summary
MNSV induces extensive reorganization of host mitochondria that provides a protected environment for viral replication.
- Taxon
- MelonCucumis melot0
- Interactor
- MNSVMelon necrotic spot viruse7pathogen
- Outcome
- extensive reorganization of host mitochondriae7
- Qualifier
- provides a protected environment to viral replicatione7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Melon necrotic spot virus”
e7“Our data document the extensive reorganization of host mitochondria induced by MNSV, which provides a protected environment to viral replication, and show that the MNSV p29 protein is the primary determinant of this effect in the host”
Claim · model-written summary
The MNSV p29 protein is the primary determinant of the reorganization of host mitochondria.
- Taxon
- MelonCucumis melot0
- Gene
- MNSV p29 proteine7
- Outcome
- reorganization of host mitochondriae7
- Qualifier
- primary determinant of this effect in the hoste7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Melon necrotic spot virus”
e7“Our data document the extensive reorganization of host mitochondria induced by MNSV, which provides a protected environment to viral replication, and show that the MNSV p29 protein is the primary determinant of this effect in the host”