Abstract
The present study is a revision of our work on evolutionary cytogenetics of the genus Zea, including several new experiments which give a deeper insight into the nature of the DNA sequences involved in telomeric regions of Zea luxurians. These new experiments, based on the Southern blotting technique and in situ hybridization, have demonstrated the following: 1) in situ hybridization (FISH) demonstrated the presence of the 180-bp repeat maize-knob-repeat-sequence in DAPI-positive terminal heterochromatic blocks of Z. luxurians (ZL-THB region); 2) Southern blot analysis confirmed that the 180-bp repeat present in maize is also present in Z. diploperennis, Z. luxurians and Tripsacum dactyloides, but not in Z. perennis; 3) another sequence with targeted sites for endonucleases, but without recognition sites for the 180-bp repeat, may be interspersed with the 180-bp repeat in a tandem array sited in the ZL-THB region; 4) in situ hybridization (GISH) of probes and blocking-probes with chromosomes of Z. luxurians (using Z. luxurians as a probe and Z. diploperennis or Z. perennis as a blocking-probe) gave strong fluorescence in both cases. Since Z. diploperennis possesses the 180-bp repeat, fluorescence on Z. luxurians chromosomes was not expected. These results can be explained if the ZL-THB regions are composed not only of 180-bp repeats interspersed with other sequences, but also of other tandem arrays unique to Z. luxurians, which, according to our GISH results, are probably located at the subterminal position.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 9
Claim · model-written summary
The 180-bp repeat occurred in terminal heterochromatin of Z. luxurians.
- Taxon
- Z. luxuriansZea luxurianse0
- Organ / stage
- terminal heterochromatic blockse0
- Outcome
- presence of the 180-bp repeat maize-knob-repeat-sequencee0
Verbatim
e0“in situ hybridization (FISH) demonstrated the presence of the 180-bp repeat maize-knob-repeat-sequence in DAPI-positive terminal heterochromatic blocks of Z. luxurians (ZL-THB region)”
Claim · model-written summary
The 180-bp repeat was present in maize.
- Outcome
- 180-bp repeat presente0
Zea maysOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the 180-bp repeat present in maize is also present in Z. diploperennis, Z. luxurians and Tripsacum dactyloides, but not in Z. perennis”
Claim · model-written summary
The 180-bp repeat was present in Z. diploperennis.
- Taxon
- Z. diploperennis
Zea diploperennise0 - Outcome
- 180-bp repeate0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the 180-bp repeat present in maize is also present in Z. diploperennis, Z. luxurians and Tripsacum dactyloides, but not in Z. perennis”
Claim · model-written summary
The 180-bp repeat was present in Z. luxurians.
- Taxon
- Z. luxuriansZea luxurianse0
- Outcome
- 180-bp repeate0
Verbatim
e0“the 180-bp repeat present in maize is also present in Z. diploperennis, Z. luxurians and Tripsacum dactyloides, but not in Z. perennis”
Claim · model-written summary
The 180-bp repeat was present in Tripsacum dactyloides.
- Taxon
- Tripsacum dactyloides
Tripsacum dactyloidese0 - Outcome
- 180-bp repeate0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the 180-bp repeat present in maize is also present in Z. diploperennis, Z. luxurians and Tripsacum dactyloides, but not in Z. perennis”
Claim · model-written summary
The 180-bp repeat was absent in Z. perennis.
- Taxon
- Z. perennis
Zea perennise0 - Outcome
- not in Z. perennise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the 180-bp repeat present in maize is also present in Z. diploperennis, Z. luxurians and Tripsacum dactyloides, but not in Z. perennis”
Claim · model-written summary
A sequence lacking 180-bp recognition sites may be interspersed with the repeat in Z. luxurians.
- Taxon
- Zea luxuriansZea luxurianse1
- Outcome
- may be interspersed with the 180-bp repeate0
- Qualifier
- another sequence with targeted sites for endonucleasese0
Verbatim
e0“another sequence with targeted sites for endonucleases, but without recognition sites for the 180-bp repeat, may be interspersed with the 180-bp repeat in a tandem array sited in the ZL-THB region”
e1“telomeric regions of Zea luxurians”
Claim · model-written summary
GISH gave strong fluorescence on Z. luxurians chromosomes with either blocking probe.
- Taxon
- Z. luxuriansZea luxurianse0
- Treatment
- Z. diploperennis or Z. perennis as a blocking-probee0
- Outcome
- strong fluorescencee0
Verbatim
e0“in situ hybridization (GISH) of probes and blocking-probes with chromosomes of Z. luxurians (using Z. luxurians as a probe and Z. diploperennis or Z. perennis as a blocking-probe) gave strong fluorescence in both cases”
Claim · model-written summary
The authors infer unique subterminal tandem arrays in Z. luxurians.
- Taxon
- Z. luxuriansZea luxurianse0
- Outcome
- other tandem arrays unique to Z. luxurianse0
- Qualifier
- probably located at the subterminal positione0
Verbatim
e0“other tandem arrays unique to Z. luxurians, which, according to our GISH results, are probably located at the subterminal position”