Abstract
The global population is expected to grow from 6 to 8 billion people and rice consumers are projected to increase by 1.8% annually until 2020. Hence, rice production must be increased between 25-45% to fulfill the growing need. Efforts to genetically improve rice for high quality grains are extensively being carried out. The cloning vectors containing the ipt gene driven by the glutenin high molecular weight promoter were successfully constructed in pCAMBIA1305.2 and transformed into A. tumefaciens LBA4404, which were then used in the genetic transformation of a japonica suspension cell culture. The highest percentage of transformation frequency based on GUS activity was 93% in the variety Hayahishiki and 77% in Nippon Bare when 200 µM AS was included in the inoculation media. The highest percentage of GUS activity was 30% in the variety Fujisaka 5 in the presence of 100 µM AS. There was no difference in terms of GUS expression when different inoculation times were tested. A twenty minute post-dehydration treatment led to the highest GUS activity in all varieties tested. The inclusion of AS is critical and very important to obtain successful transformation. The sensitivity and response of suspension cells to different hygromycin concentrations was varied among the varieties tested. Selection of transformed cells in N6 liquid media containing 25 mg/L hygromycin proved to be easy and facilitated the removal of non-transformed cells. PCR analysis has shown that 2.3% of the putatively transformed rice variety Nippon Bare contained the ipt gene, while only 2.0% for the Hayahishiki variety. The finding of this research shows the potential for rice suspension cells in regeneration and genetic transformation systems by providing continuous explants and could be used as tools to obtain large scale transformation of rice plants via Agrobacterium tumefaciens.
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Claims 12
Claim · model-written summary
The cloning vectors containing the ipt gene driven by the glutenin high molecular weight promoter were successfully constructed in pCAMBIA1305.2 and transformed into A. tumefaciens LBA4404
- Taxon
- rice
Oryza sativat0 - Interactor
- A. tumefaciens LBA4404
Agrobacterium tumefaciense0other - Gene
- ipt genee0
- Outcome
- cloning vectors containing the ipt genee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The cloning vectors containing the ipt gene driven by the glutenin high molecular weight promoter were successfully constructed in pCAMBIA1305.2 and transformed into A. tumefaciens LBA4404”
t0“rice”
Claim · model-written summary
The highest percentage of transformation frequency based on GUS activity was 93% in the variety Hayahishiki and 77% in Nippon Bare when 200 µM AS was included in the inoculation media
- Taxon
- riceOryza sativat0
- Material
- Hayahishikie0
- Treatment
- 200 µM ASe0
- Outcome
- transformation frequency based on GUS activitye0
- Direction
- higheste0
- Value
- 93%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest percentage of transformation frequency based on GUS activity was 93% in the variety Hayahishiki and 77% in Nippon Bare when 200 µM AS was included in the inoculation media”
t0“rice”
Claim · model-written summary
Nippon Bare transformation frequency was 77% with 200 µM AS.
- Taxon
- riceOryza sativat0
- Material
- Nippon Baree0
- Treatment
- 200 µM ASe0
- Outcome
- transformation frequency based on GUS activitye0
- Direction
- higheste0
- Value
- 77%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest percentage of transformation frequency based on GUS activity was 93% in the variety Hayahishiki and 77% in Nippon Bare when 200 µM AS was included in the inoculation media”
t0“rice”
Claim · model-written summary
The highest percentage of GUS activity was 30% in the variety Fujisaka 5 in the presence of 100 µM AS
- Taxon
- riceOryza sativat0
- Material
- Fujisaka 5e0
- Treatment
- 100 µM ASe0
- Outcome
- GUS activitye0
- Direction
- higheste0
- Value
- 30%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest percentage of GUS activity was 30% in the variety Fujisaka 5 in the presence of 100 µM AS”
t0“rice”
Claim · model-written summary
There was no difference in terms of GUS expression when different inoculation times were tested
- Taxon
- riceOryza sativat0
- Treatment
- different inoculation timese0
- Outcome
- GUS expressione0
- Direction
- no differencee0
- Comparator
- different inoculation timese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“There was no difference in terms of GUS expression when different inoculation times were tested”
t0“rice”
Claim · model-written summary
A twenty minute post-dehydration treatment led to the highest GUS activity in all varieties tested
- Taxon
- riceOryza sativat0
- Treatment
- twenty minute post-dehydration treatmente0
- Outcome
- GUS activitye0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A twenty minute post-dehydration treatment led to the highest GUS activity in all varieties tested”
t0“rice”
Claim · model-written summary
The inclusion of AS is critical and very important to obtain successful transformation
- Taxon
- riceOryza sativat0
- Outcome
- inclusion of AS is critical and very important to obtain successful transformatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The inclusion of AS is critical and very important to obtain successful transformation”
t0“rice”
Claim · model-written summary
The sensitivity and response of suspension cells to different hygromycin concentrations was varied among the varieties tested
- Taxon
- riceOryza sativat0
- Material
- varieties testede0
- Treatment
- different hygromycin concentrationse0
- Outcome
- sensitivity and response of suspension cellse0
- Direction
- variede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The sensitivity and response of suspension cells to different hygromycin concentrations was varied among the varieties tested”
t0“rice”
Claim · model-written summary
Selection of transformed cells in N6 liquid media containing 25 mg/L hygromycin proved to be easy and facilitated the removal of non-transformed cells
- Taxon
- riceOryza sativat0
- Treatment
- N6 liquid media containing 25 mg/L hygromycine0
- Outcome
- removal of non-transformed cellse0
- Direction
- facilitatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Selection of transformed cells in N6 liquid media containing 25 mg/L hygromycin proved to be easy and facilitated the removal of non-transformed cells”
t0“rice”
Claim · model-written summary
2.3% of the putatively transformed rice variety Nippon Bare contained the ipt gene
- Taxon
- riceOryza sativae0
- Material
- Nippon Baree0
- Gene
- ipt genee0
- Outcome
- contained the ipt genee0
- Value
- 2.3%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“2.3% of the putatively transformed rice variety Nippon Bare contained the ipt gene”
Claim · model-written summary
Only 2.0% of putatively transformed Hayahishiki contained ipt.
- Taxon
- riceOryza sativae0
- Material
- Hayahishikie0
- Gene
- ipt genee0
- Outcome
- putatively transformed ricee0
- Value
- 2.0%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“2.3% of the putatively transformed rice variety Nippon Bare contained the ipt gene, while only 2.0% for the Hayahishiki variety”
Claim · model-written summary
rice suspension cells in regeneration and genetic transformation systems by providing continuous explants and could be used as tools to obtain large scale transformation of rice plants
- Taxon
- riceOryza sativae0
- Outcome
- rice suspension cells in regeneration and genetic transformation systemse0
- Value
- large scalee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“rice suspension cells in regeneration and genetic transformation systems by providing continuous explants and could be used as tools to obtain large scale transformation of rice plants”
The same questions in other papers
inoculation · molecular: expression · Oryza sativa (rice)Positive and negative results
4 papers in the same direction as this paper · 51 reporting no difference from an alternative
- positive
- positive
- positive
52 papers more in this group
See all 56 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.