Claim · model-written summary
2.3% of the putatively transformed rice variety Nippon Bare contained the ipt gene
- Taxon
- rice
Oryza sativae0
- Material
- Nippon Baree0
- Gene
- ipt genee0
- Outcome
- contained the ipt genee0
- Value
- 2.3%e0
Not reportedCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleTreatmentComparatorSettingDirectionQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“2.3% of the putatively transformed rice variety Nippon Bare contained the ipt gene”
Title
Alina Wagiran; Ismanizan Ismail; Che Radziah Che Mohd Zain; et al. Australian Journal of Crop Science 2010
Other claims from this paper (11)
- Observation The cloning vectors containing the ipt gene driven by the glutenin high molecular weight promoter were successfully constructed in pCAMBIA1305.2 and transformed into A. tumefaciens LBA4404
- Intervention effect The highest percentage of transformation frequency based on GUS activity was 93% in the variety Hayahishiki and 77% in Nippon Bare when 200 µM AS was included in the inoculation media
- Intervention effect Nippon Bare transformation frequency was 77% with 200 µM AS.
- Intervention effect The highest percentage of GUS activity was 30% in the variety Fujisaka 5 in the presence of 100 µM AS
- Intervention effect There was no difference in terms of GUS expression when different inoculation times were tested
- Intervention effect A twenty minute post-dehydration treatment led to the highest GUS activity in all varieties tested
- Recommendation The inclusion of AS is critical and very important to obtain successful transformation
- Association The sensitivity and response of suspension cells to different hygromycin concentrations was varied among the varieties tested
- Intervention effect Selection of transformed cells in N6 liquid media containing 25 mg/L hygromycin proved to be easy and facilitated the removal of non-transformed cells
- Observation Only 2.0% of putatively transformed Hayahishiki contained ipt.
- Method / resource rice suspension cells in regeneration and genetic transformation systems by providing continuous explants and could be used as tools to obtain large scale transformation of rice plants