Abstract
Standardization is an important step for the establishment of a consistent biological activity, a consistent chemical profile, or simply a quality assurance program for production and manufacturing of herbal drugs preparation of any herbal formulation identification, evaluation and standardization is rudimentary identification involves the morphology, microscopy parameter of plants, evaluation and standardization of herbal drugs includes physical, chemical and biological parameters. These parameters are crucial for preparation of accurate and potent formulation. The present communication attempts to investigate pharmacognostical and phytochemical details of Cucurbita maxima, (Cucurbitaceae). The Preliminary phytochemical analysis revealed presence of carbohydrates, alkaloids, flavonoids, saponins, proteins and amino acids in alcoholic extract. HPTLC studies reveal that alcoholic extract gives 3 spots and alcoholic extract depicts 5 spots on the TLC plate in Butanol: acetic: water solvent system with Ninhydrin as spraying agent and 3 spots with vanillin as spraying agent and with butanol: phenol: water (6:1:1) 4 spots were seen with Ninhydrin as spraying agent and 2 spots were seen with vanillin as spraying agents. The GC/MS of pet ether methyl ester showed number of peaks. Out of which 3 highest peaks in descending order were taken into consideration. OSAZONE were formed which showed needle shaped crystals of glucaosazone. The study revealed specific identities for which may play a key role in identification of plant and can be useful in standardization of the herbal drugs.
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Claims 10
Claim · model-written summary
Preliminary phytochemical analysis of the Cucurbita maxima seed alcoholic extract revealed carbohydrates, alkaloids and flavonoids.
- Taxon
- Cucurbita Maxima
Cucurbita maximat0 - Organ / stage
- Seedt0
- Outcome
- presence of carbohydrates, alkaloids, flavonoidse0
- Qualifier
- alcoholic extracte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Standardization of Ethanolic Extract of Cucurbita Maxima Seed”
e0“The Preliminary phytochemical analysis revealed presence of carbohydrates, alkaloids, flavonoids, saponins, proteins and amino acids in alcoholic extract”
Claim · model-written summary
Preliminary phytochemical analysis of the Cucurbita maxima seed alcoholic extract revealed saponins, proteins and amino acids.
- Taxon
- Cucurbita MaximaCucurbita maximat0
- Organ / stage
- Seedt0
- Outcome
- saponins, proteins and amino acidse0
- Qualifier
- alcoholic extracte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Standardization of Ethanolic Extract of Cucurbita Maxima Seed”
e0“The Preliminary phytochemical analysis revealed presence of carbohydrates, alkaloids, flavonoids, saponins, proteins and amino acids in alcoholic extract”
Claim · model-written summary
The alcoholic extract gave 3 spots on the TLC plate in the butanol: acetic: water system with ninhydrin.
- Taxon
- Cucurbita MaximaCucurbita maximat0
- Outcome
- spots on the TLC platee1
- Value
- 3 spotse1
- Qualifier
- Butanol: acetic: water solvent system with Ninhydrin as spraying agente1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Standardization of Ethanolic Extract of Cucurbita Maxima Seed”
e1“HPTLC studies reveal that alcoholic extract gives 3 spots and alcoholic extract depicts 5 spots on the TLC plate in Butanol: acetic: water solvent system with Ninhydrin as spraying agent”
Claim · model-written summary
The alcoholic extract depicted 5 spots on the TLC plate in the butanol: acetic: water system with ninhydrin.
- Taxon
- Cucurbita MaximaCucurbita maximat0
- Outcome
- spots on the TLC platee1
- Value
- 5 spotse1
- Qualifier
- Butanol: acetic: water solvent system with Ninhydrin as spraying agente1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Standardization of Ethanolic Extract of Cucurbita Maxima Seed”
e1“HPTLC studies reveal that alcoholic extract gives 3 spots and alcoholic extract depicts 5 spots on the TLC plate in Butanol: acetic: water solvent system with Ninhydrin as spraying agent”
Claim · model-written summary
In the butanol: phenol: water (6:1:1) system with ninhydrin, the extract gave 4 spots.
- Taxon
- Cucurbita MaximaCucurbita maximat0
- Outcome
- spots were seene2
- Value
- 4 spotse2
- Qualifier
- butanol: phenol: water (6:1:1)e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Standardization of Ethanolic Extract of Cucurbita Maxima Seed”
e2“butanol: phenol: water (6:1:1) 4 spots were seen with Ninhydrin as spraying agent”
Claim · model-written summary
The extract gave 3 spots on the TLC plate with vanillin as spraying agent.
- Taxon
- Cucurbita MaximaCucurbita maximat0
- Outcome
- spotse3
- Value
- 3 spotse3
- Qualifier
- vanillin as spraying agente3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Standardization of Ethanolic Extract of Cucurbita Maxima Seed”
e3“3 spots with vanillin as spraying agent”
Claim · model-written summary
In the butanol: phenol: water (6:1:1) system with vanillin, the extract gave 2 spots.
- Taxon
- Cucurbita MaximaCucurbita maximat0
- Outcome
- spots were seene4
- Value
- 2 spotse4
- Qualifier
- vanillin as spraying agentse4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Standardization of Ethanolic Extract of Cucurbita Maxima Seed”
e4“2 spots were seen with vanillin as spraying agents”
Claim · model-written summary
Osazone formation gave needle-shaped crystals of glucaosazone from the Cucurbita maxima seed extract.
- Taxon
- Cucurbita MaximaCucurbita maximat0
- Outcome
- needle shaped crystals of glucaosazonee5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Standardization of Ethanolic Extract of Cucurbita Maxima Seed”
e5“OSAZONE were formed which showed needle shaped crystals of glucaosazone”
Claim · model-written summary
GC/MS of the pet ether methyl ester of the Cucurbita maxima seed extract showed a number of peaks.
- Taxon
- Cucurbita MaximaCucurbita maximat0
- Outcome
- number of peakse6
- Qualifier
- pet ether methyl estere6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Standardization of Ethanolic Extract of Cucurbita Maxima Seed”
e6“The GC/MS of pet ether methyl ester showed number of peaks”
Claim · model-written summary
The extract fingerprint identities may help standardization of herbal drugs made from Cucurbita maxima.
- Taxon
- Cucurbita MaximaCucurbita maximat0
- Outcome
- standardization of the herbal drugse7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Standardization of Ethanolic Extract of Cucurbita Maxima Seed”
e7“The study revealed specific identities for which may play a key role in identification of plant and can be useful in standardization of the herbal drugs”