Abstract
Natural mutations in translation initiation factor eIF4E confer resistance to potyviruses in many plant species. Potato is a staple food crop plagued by several potyviruses, yet to date no known eIF4E-mediated resistance genes have been identified. In this study, we demonstrate that transgenic expression of the pvr1(2) gene from pepper confers resistance to Potato virus Y (PVY) in potato. We then use this information to convert the susceptible potato ortholog of this allele into a de novo allele for resistance to PVY using site-directed mutagenesis. Potato plants overexpressing the mutated potato allele are resistant to virus infection. Resistant lines expressed high levels of eIF4E mRNA and protein. The resistant plants showed growth similar to untransformed controls and produced phenotypically similar tubers. This technique disrupts a key step in the viral infection process and may potentially be used to engineer virus resistance in a number of economically important plant-viral pathosystems. Furthermore, the general public may be more amenable to the 'intragenic' nature of this approach because the transferred coding region is modified from a gene in the target crop rather than from a distant species.
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Claims 6
Claim · model-written summary
Transgenic pepper pvr1(2) expression conferred PVY resistance in potato.
- Taxon
- potato
Solanum tuberosume0 - Treatment
- transgenic expression of the pvr1(2) gene from peppere0
- Interactor
- Potato virus Y (PVY)
Potato virus Ye0pathogen - Gene
- pvr1(2) genee0
- Outcome
- resistance to Potato virus Y (PVY)e0
- Direction
- conferse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transgenic expression of the pvr1(2) gene from pepper confers resistance to Potato virus Y (PVY) in potato”
Claim · model-written summary
A susceptible potato ortholog was converted into a new PVY-resistance allele by site-directed mutagenesis.
- Taxon
- potatoSolanum tuberosume0
- Gene
- potato orthologe0
- Outcome
- de novo allele for resistance to PVYe0
- Comparator
- susceptible potato orthologe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“convert the susceptible potato ortholog of this allele into a de novo allele for resistance to PVY using site-directed mutagenesis”
Claim · model-written summary
Potato plants overexpressing the mutated allele resisted virus infection.
- Taxon
- PotatoSolanum tuberosume0
- Treatment
- overexpressing the mutated potato allelee0
- Interactor
- viruse0pathogen
- Outcome
- virus infectione0
- Direction
- resistante0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Potato plants overexpressing the mutated potato allele are resistant to virus infection.”
Claim · model-written summary
Resistant lines expressed high eIF4E mRNA and protein levels.
- Taxon
- potatoSolanum tuberosumt0
- Material
- Resistant linese0
- Gene
- eIF4Ee0
- Outcome
- levels of eIF4E mRNA and proteine0
- Direction
- highe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Resistant lines expressed high levels of eIF4E mRNA and protein.”
t0“potato”
Claim · model-written summary
Resistant potato plants grew similarly to untransformed controls.
- Taxon
- potatoSolanum tuberosumt0
- Material
- resistant plantse0
- Outcome
- growthe0
- Direction
- similare0
- Comparator
- untransformed controlse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The resistant plants showed growth similar to untransformed controls and produced phenotypically similar tubers.”
t0“potato”
Claim · model-written summary
Resistant potato plants produced tubers phenotypically similar to controls.
- Taxon
- potatoSolanum tuberosumt0
- Material
- resistant plantse0
- Organ / stage
- tuberse0
- Outcome
- phenotypically similar tuberse0
- Direction
- similare0
- Comparator
- untransformed controlse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The resistant plants showed growth similar to untransformed controls and produced phenotypically similar tubers.”
t0“potato”