Abstract
SAG101, a leaf senescence-associated gene, was cloned from an Arabidopsis leaf senescence enhancer trap line and functionally characterized. Reporter gene and RNA gel blot analyses revealed that SAG101 was not expressed until the onset of senescence in leaves. A recombinant SAG101 fusion protein overexpressed in Escherichia coli displayed acyl hydrolase activity. Antisense RNA interference in transgenic plants delayed the onset of leaf senescence for approximately 4 days. Chemically induced overexpression of SAG101 caused precocious senescence in both attached and detached leaves of transgenic Arabidopsis plants. These data suggest that SAG101 plays a significant role in leaf senescence.
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Claims 4
Claim · model-written summary
SAG101 expression began at leaf senescence onset.
- Taxon
- Arabidopsis
Arabidopsist0 - Organ / stage
- leavese0
- Gene
- SAG101e0
- Outcome
- not expressed until the onset of senescencee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Reporter gene and RNA gel blot analyses revealed that SAG101 was not expressed until the onset of senescence in leaves.”
t0“Arabidopsis”
Claim · model-written summary
Recombinant SAG101 displayed acyl hydrolase activity.
- Taxon
- Escherichia coli
.jpg?width=60)
Escherichia colie0 - Gene
- SAG101e0
- Outcome
- acyl hydrolase activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“A recombinant SAG101 fusion protein overexpressed in Escherichia coli displayed acyl hydrolase activity.”
Claim · model-written summary
Antisense interference delayed leaf senescence by approximately 4 days.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- Antisense RNA interferencee0
- Outcome
- onset of leaf senescencee0
- Direction
- delayede0
- Value
- approximately 4 dayse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Antisense RNA interference in transgenic plants delayed the onset of leaf senescence for approximately 4 days.”
t0“Arabidopsis”
Claim · model-written summary
Induced SAG101 overexpression caused precocious senescence in attached and detached Arabidopsis leaves.
- Taxon
- ArabidopsisArabidopsise0
- Organ / stage
- attached and detached leavese0
- Treatment
- Chemically induced overexpression of SAG101e0
- Gene
- SAG101e0
- Outcome
- precocious senescencee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Chemically induced overexpression of SAG101 caused precocious senescence in both attached and detached leaves of transgenic Arabidopsis plants.”
The same questions in other papers
loss of function · quality: postharvest · ArabidopsisPositive and negative results
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See all 26 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.