Abstract
The influence of different antibiotic treatments in soybean genetic transformation was evaluated. First, an assay was performed to verify how different antibiotic treatments affect soybean embryogenic tissues. The effect of carbenicillin at 500 mg L-1 was genotype-dependent. This antibiotic did not affect embryo survival of cv. IAS5, but a three-fold increase of embryo proliferation was observed for cv. Bragg, when compared to the control. On the other hand, cefotaxime at 350 and 500 mg L-1 caused death of embryogenic tissues of both cultivars. Finally, the association of cefotaxime (250 mg L-1) + vancomycin (250 mg L-1) did not affect negatively the somatic embryos of tested cultivars until 63 days of treatment. Thereafter, a second experiment was carried out to determine the efficacy of different antibiotic treatments in suppressing LBA4404 Agrobacterium tumefaciens strain in genetic transformation. On tissue culture conditions, carbenicillin at 500 and 1000 mg L-1 was not active against Agrobacterium. On the other hand, treatments with cefotaxime at 350 and 500 mg L-1, and cefotaxime + vancomycin efficiently suppressed Agrobacterium during 49 days. Data of both experiments suggested cefotaxime + vancomycin for 49-63 days as the most appropriate treatment. This is the first work reporting the effect of antibiotics on soybean tissues. By identifying an antibiotic combination that suppressed A. tumefaciens with minimal phytotoxic effects, we are able to recommend it for improvement of soybean Agrobacterium-mediated transformation procedure.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by-nc. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 9
Claim · model-written summary
Carbenicillin had a genotype dependent effect on soybean embryogenic tissue.
Not reportedTaxon
Verbatim
e0“The effect of carbenicillin at 500 mg L-1 was genotype-dependent”
Claim · model-written summary
Carbenicillin did not affect embryo survival in IAS5.
Not reportedTaxonComparator
Verbatim
e0“This antibiotic did not affect embryo survival of cv. IAS5”
Claim · model-written summary
Carbenicillin tripled embryo proliferation in Bragg versus control.
- Material
- cv. Bragge0
- Outcome
- embryo proliferatione0
- Direction
- increasee0
- Value
- three-folde0
- Comparator
- the controle0
Not reportedTaxonTreatment
Verbatim
e0“a three-fold increase of embryo proliferation was observed for cv. Bragg, when compared to the control”
Claim · model-written summary
Cefotaxime at 350 and 500 mg L-1 killed embryogenic tissue in both cultivars.
- Treatment
- cefotaxime at 350 and 500 mg L-1e0
- Outcome
- death of embryogenic tissuese0
- Direction
- caused deathe0
Not reportedTaxonComparator
Verbatim
e0“cefotaxime at 350 and 500 mg L-1 caused death of embryogenic tissues of both cultivars”
Claim · model-written summary
Cefotaxime plus vancomycin did not harm somatic embryos through 63 days.
- Treatment
- cefotaxime (250 mg L-1) + vancomycin (250 mg L-1)e0
- Outcome
- somatic embryose0
- Direction
- did not affect negativelye0
- Qualifier
- until 63 days of treatmente0
Not reportedTaxonComparator
Verbatim
e0“cefotaxime (250 mg L-1) + vancomycin (250 mg L-1) did not affect negatively the somatic embryos of tested cultivars until 63 days of treatment”
Claim · model-written summary
Carbenicillin at 500 and 1000 mg L-1 did not suppress Agrobacterium in tissue culture.
- Treatment
- carbenicillin at 500 and 1000 mg L-1e0
- Interactor
- Agrobacterium
.jpg?width=60)
Agrobacteriume0other - Outcome
- Agrobacteriume0
- Direction
- not activee0
Not reportedTaxonComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“carbenicillin at 500 and 1000 mg L-1 was not active against Agrobacterium”
Claim · model-written summary
Cefotaxime at 350 and 500 mg L-1 suppressed Agrobacterium for 49 days.
- Treatment
- cefotaxime at 350 and 500 mg L-1e0
- Interactor
- AgrobacteriumAgrobacteriume0other
- Outcome
- Agrobacteriume0
- Direction
- suppressede0
Not reportedTaxonComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cefotaxime at 350 and 500 mg L-1, and cefotaxime + vancomycin efficiently suppressed Agrobacterium during 49 days”
Claim · model-written summary
Cefotaxime plus vancomycin suppressed Agrobacterium for 49 days.
- Treatment
- cefotaxime + vancomycine0
- Interactor
- AgrobacteriumAgrobacteriume0other
- Outcome
- Agrobacteriume0
- Direction
- suppressede0
Not reportedTaxonComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cefotaxime + vancomycin efficiently suppressed Agrobacterium during 49 days”
Claim · model-written summary
The authors recommend cefotaxime plus vancomycin for 49 to 63 days in soybean transformation.
- Outcome
- cefotaxime + vancomycin for 49-63 dayse0
Not reportedTaxon
Verbatim
e0“Data of both experiments suggested cefotaxime + vancomycin for 49-63 days as the most appropriate treatment”