Abstract
The anionic peroxidase associated with the suberization response in potato (Solanum tuberosum L.) tubers during wound healing has been purified and partially characterized at the biochemical level. It is a 45-kD, class III (plant secretory) peroxidase that is localized to suberizing tissues and shows a preference for feruloyl (o-methoxyphenol)-substituted substrates (order of substrate preference: feruloyl > caffeoyl > p-coumaryl approximately syringyl) such as those that accumulate in tubers during wound healing. There was little influence on oxidation by side chain derivatization, although hydroxycinnamates were preferred over the corresponding hydroxycinnamyl alcohols. The substrate specificity pattern is consistent with the natural substrate incorporation into potato wound suberin. In contrast, the cationic peroxidase(s) induced in response to wound healing in potato tubers is present in both suberizing and nonsuberizing tissues and does not discriminate between hydroxycinnamates and hydroxycinnamyl alcohols. A synthetic polymer prepared using E-[8-(13)C]ferulic acid, H(2)O(2), and the purified anionic enzyme contained a significant amount of cross-linking through C-8, albeit with retention of unsaturation.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 9
Claim · model-written summary
The potato wound-associated anionic peroxidase was purified and characterized as a 45-kD class III enzyme.
- Taxon
- Potato
Solanum tuberosumt0 - Gene
- peroxidasee0
- Outcome
- 45-kD, class III (plant secretory) peroxidasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“It is a 45-kD, class III (plant secretory) peroxidase”
t0“Potato”
Claim · model-written summary
The anionic peroxidase localized to suberizing tissues.
- Taxon
- PotatoSolanum tuberosumt0
- Gene
- Peroxidaset1
- Outcome
- localized to suberizing tissuese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“localized to suberizing tissues”
t0“Potato”
t1“Peroxidase”
Claim · model-written summary
The anionic peroxidase preferred feruloyl substrates over caffeoyl, p-coumaryl and syringyl substrates.
- Taxon
- PotatoSolanum tuberosumt0
- Gene
- Peroxidaset1
- Outcome
- substrate preferencee0
- Direction
- preferencee0
- Qualifier
- feruloyl > caffeoyl > p-coumaryl approximately syringyle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“shows a preference for feruloyl (o-methoxyphenol)-substituted substrates (order of substrate preference: feruloyl > caffeoyl > p-coumaryl approximately syringyl)”
t0“Potato”
t1“Peroxidase”
Claim · model-written summary
Side chain derivatization had little influence on oxidation.
- Taxon
- PotatoSolanum tuberosumt0
- Outcome
- oxidatione0
- Direction
- little influencee0
- Qualifier
- side chain derivatizatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“There was little influence on oxidation by side chain derivatization”
t0“Potato”
Claim · model-written summary
Hydroxycinnamates were preferred over their corresponding alcohols.
- Taxon
- PotatoSolanum tuberosumt0
- Outcome
- hydroxycinnamates were preferrede0
- Direction
- preferrede0
- Comparator
- the corresponding hydroxycinnamyl alcoholse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“hydroxycinnamates were preferred over the corresponding hydroxycinnamyl alcohols”
t0“Potato”
Claim · model-written summary
The substrate specificity pattern was consistent with natural substrate incorporation into potato wound suberin.
- Taxon
- potatoSolanum tuberosume0
- Organ / stage
- wound suberine0
- Outcome
- natural substrate incorporation into potato wound suberine0
- Qualifier
- substrate specificity patterne0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The substrate specificity pattern is consistent with the natural substrate incorporation into potato wound suberin”
Claim · model-written summary
Cationic peroxidases occurred in both suberizing and nonsuberizing potato tissues.
- Taxon
- potatoSolanum tuberosume0
- Organ / stage
- tuberse0
- Gene
- cationic peroxidase(s)e0
- Outcome
- present in both suberizing and nonsuberizing tissuese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the cationic peroxidase(s) induced in response to wound healing in potato tubers is present in both suberizing and nonsuberizing tissues”
Claim · model-written summary
Cationic peroxidases did not discriminate between hydroxycinnamates and their alcohols.
- Taxon
- PotatoSolanum tuberosumt0
- Gene
- Peroxidaset1
- Outcome
- does not discriminate between hydroxycinnamates and hydroxycinnamyl alcoholse0
- Direction
- does not discriminatee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“does not discriminate between hydroxycinnamates and hydroxycinnamyl alcohols”
t0“Potato”
t1“Peroxidase”
Claim · model-written summary
The polymer made with purified anionic peroxidase showed substantial C-8 cross-linking while retaining unsaturation.
- Taxon
- PotatoSolanum tuberosumt0
- Outcome
- cross-linking through C-8e0
- Qualifier
- retention of unsaturatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A synthetic polymer prepared using E-[8-(13)C]ferulic acid, H(2)O(2), and the purified anionic enzyme contained a significant amount of cross-linking through C-8, albeit with retention of unsaturation”
t0“Potato”