Abstract
Cowpea mosaic virus (CPMV) replicates in close association with small membranous vesicles that are formed by rearrangements of intracellular membranes. To determine which of the viral proteins are responsible for the rearrangements of membranes and the attachment of the replication complex, we have expressed individual CPMV proteins encoded by RNA1 in cowpea protoplasts by transient expression and in Nicotiana benthamiana plants by using the tobacco rattle virus (TRV) expression vector. The 32-kDa protein (32K) and 60K, when expressed individually, accumulate in only low amounts but are found associated with membranes mainly derived from the endoplasmic reticulum (ER). 24K and 110K are freely soluble and accumulate to high levels. With the TRV vector, expression of 32K and 60K results in rearrangement of ER membranes. Besides, expression of 32K and 60K results in necrosis of the inoculated N. benthamiana leaves, suggesting that 32K and 60K are cytotoxic proteins. On the other hand, during CPMV infection 32K and 60K accumulate to high levels without causing necrosis.
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Claims 7
Claim · model-written summary
When expressed individually, the CPMV 32K and 60K proteins accumulated only in low amounts.
Not reportedTaxon
Verbatim
e0“The 32-kDa protein (32K) and 60K, when expressed individually, accumulate in only low amounts”
Claim · model-written summary
The individually expressed CPMV 32K and 60K proteins were found associated with membranes mainly derived from the endoplasmic reticulum.
- Outcome
- associated with membranes mainly derived from the endoplasmic reticulume0
Not reportedTaxon
Verbatim
e0“are found associated with membranes mainly derived from the endoplasmic reticulum (ER)”
Claim · model-written summary
The CPMV 24K and 110K proteins were freely soluble and accumulated to high levels.
Not reportedTaxon
Verbatim
e0“24K and 110K are freely soluble and accumulate to high levels”
Claim · model-written summary
With the TRV vector in N. benthamiana, expression of 32K and 60K resulted in rearrangement of ER membranes.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae1 - Treatment
- expression of 32K and 60Ke0
- Outcome
- rearrangement of ER membranese0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression of 32K and 60K results in rearrangement of ER membranes”
e1“Nicotiana benthamiana plants by using the tobacco rattle virus (TRV) expression vector”
Claim · model-written summary
Expression of 32K and 60K caused necrosis of the inoculated N. benthamiana leaves.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Organ / stage
- leavese0
- Treatment
- expression of 32K and 60Ke0
- Outcome
- necrosise0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression of 32K and 60K results in necrosis of the inoculated N. benthamiana leaves”
Claim · model-written summary
The authors suggest that 32K and 60K are cytotoxic proteins, based on the necrosis they caused.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Gene
- 32K and 60Ke0
- Outcome
- necrosise0
- Qualifier
- cytotoxic proteinse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression of 32K and 60K results in necrosis of the inoculated N. benthamiana leaves, suggesting that 32K and 60K are cytotoxic proteins”
Claim · model-written summary
During CPMV infection, 32K and 60K accumulated to high levels without causing necrosis.
- Interactor
- CPMVCowpea mosaic viruse0pathogen
- Gene
- 32K and 60Ke0
- Outcome
- accumulate to high levels without causing necrosise0
Not reportedTaxon
Verbatim
e0“during CPMV infection 32K and 60K accumulate to high levels without causing necrosis”
The same questions in other papers
transgenic · disease · Nicotiana benthamianaPositive and negative results
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See all 53 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.