Abstract
Kim, W. B., Lim, C. J., Jang, H. A., Yi, S. Y., Oh, S.-K., Lee, H. Y., Kim, H. A., Park, Y.-I. and Kwon, S.-Y. 2014. SlPMEI, a pollen-specific gene in tomato. Can. J. Plant Sci. 94: 73–83. Pectin is one of the main components of plant cell walls, and its biosynthesis is controlled by pectin methylesterase (PME). Pectin methylesterase inhibitors (PMEIs) are key regulators of PME. We report here the cloning and characterization of a novel Solanum lycopersicum L. PMEI gene, SlPMEI. RT-PCR studies of leaf, seed, fruit, flower, and flower organs confirmed that SlPMEI is expressed specifically in pollen. Promoter analysis of SlPMEI revealed pollen-specific cis-acting elements (pollen lat52 and g10). In addition, SlPMEI is expressed independently of abiotic stress, pathogen exposure, and growth stage in tomato, and a histochemical analysis of promoter activity revealed pollen-specific expression in both Arabidopsis and tomato. Under the microscope, we observed pollen-specific GUS expression in the stamen of transgenic tomato plant. These results indicate that the promoter of SlPMEI has strong pollen-specific activity, and could therefore be useful for development of industrially and agronomically important transgenic plants.
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Claims 9
Claim · model-written summary
The authors cloned and characterized a novel tomato PMEI gene, SlPMEI.
- Taxon
- Solanum lycopersicum L.
Solanum lycopersicume0 - Gene
- SlPMEIe0
- Outcome
- cloning and characterizatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We report here the cloning and characterization of a novel Solanum lycopersicum L. PMEI gene, SlPMEI.”
Claim · model-written summary
SlPMEI was expressed specifically in pollen.
- Taxon
- tomatoSolanum lycopersicumt0
- Organ / stage
- pollene0
- Gene
- SlPMEIe0
- Outcome
- expressed specifically in pollene0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SlPMEI is expressed specifically in pollen”
t0“tomato”
Claim · model-written summary
The SlPMEI promoter contained pollen specific cis acting elements.
- Taxon
- tomatoSolanum lycopersicumt0
- Gene
- SlPMEIe0
- Outcome
- pollen-specific cis-acting elementse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Promoter analysis of SlPMEI revealed pollen-specific cis-acting elements (pollen lat52 and g10).”
t0“tomato”
Claim · model-written summary
SlPMEI expression was independent of abiotic stress, pathogen exposure and growth stage.
- Taxon
- tomatoSolanum lycopersicume0
- Gene
- SlPMEIe0
- Outcome
- expressed independently of abiotic stress, pathogen exposure, and growth stagee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SlPMEI is expressed independently of abiotic stress, pathogen exposure, and growth stage in tomato”
Claim · model-written summary
Histochemistry showed pollen specific promoter activity in Arabidopsis.
- Taxon
- Arabidopsis
Arabidopsise0 - Organ / stage
- pollene0
- Outcome
- pollen-specific expressione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“histochemical analysis of promoter activity revealed pollen-specific expression in both Arabidopsis and tomato”
Claim · model-written summary
Histochemistry showed pollen specific promoter activity in tomato.
- Taxon
- tomatoSolanum lycopersicume0
- Organ / stage
- pollene0
- Outcome
- pollen-specific expressione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“histochemical analysis of promoter activity revealed pollen-specific expression in both Arabidopsis and tomato”
Claim · model-written summary
Transgenic tomato stamens showed pollen specific GUS expression.
- Taxon
- tomatoSolanum lycopersicume0
- Material
- transgenic tomato plante0
- Organ / stage
- stamene0
- Gene
- GUSe0
- Outcome
- pollen-specific GUS expressione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we observed pollen-specific GUS expression in the stamen of transgenic tomato plant”
Claim · model-written summary
The authors infer strong pollen specific SlPMEI promoter activity.
- Taxon
- tomatoSolanum lycopersicumt0
- Gene
- SlPMEIe0
- Outcome
- strong pollen-specific activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the promoter of SlPMEI has strong pollen-specific activity”
t0“tomato”
Claim · model-written summary
The authors suggest the promoter could aid development of transgenic plants.
- Taxon
- plantsViridiplantaee0
- Outcome
- development of industrially and agronomically important transgenic plantse0
Verbatim
e0“could therefore be useful for development of industrially and agronomically important transgenic plants”