Abstract
RNA silencing controls endogenous gene expression and drives defensive reactions against invasive nucleic acids like viruses. In plants, it has been demonstrated that RNA silencing can be transmitted through grafting between scions and silenced rootstocks to attenuate virus and viroid accumulation in the scions. This has been obtained mostly using transgenic plants, which may be a drawback in current agriculture. In the present study, we examined the dynamics of infection of a resistance-breaking strain of Tomato spotted wilt virus (RB-TSWV) through the graft between an old Apulian (southern Italy) tomato variety, denoted Sl-Ma, used as a rootstock and commercial tomato varieties used as scions. In tests with non-grafted plants, Sl-Ma showed resistance to the RB-TSWV infection as viral RNA accumulated at low levels and plants recovered from disease symptoms by 21 days post inoculation. The resistance trait was transmitted to the otherwise highly susceptible tomato genotypes grafted onto Sl-Ma. The results from the analysis of small RNAs hallmark genes involved in RNA silencing and virus-induced gene silencing suggest that RNA silencing is involved in the resistance showed by Sl-Ma against RB-TSWV and in scions grafted on this rootstock. The results from self-grafted susceptible tomato varieties suggest also that RNA silencing is enhanced by the graft itself. We can foresee interesting practical implications of the approach described in this paper.
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Claims 5
Claim · model-written summary
Non-grafted Sl-Ma resisted RB-TSWV infection with low viral RNA accumulation.
- Taxon
- tomato
Solanum lycopersicumt0 - Material
- Sl-Mae0
- Interactor
- RB-TSWVe0pathogen
- Outcome
- viral RNA accumulated at low levelse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In tests with non-grafted plants, Sl-Ma showed resistance to the RB-TSWV infection as viral RNA accumulated at low levels and plants recovered from disease symptoms by 21 days post inoculation.”
t0“Tomato Variety”
Claim · model-written summary
Non-grafted Sl-Ma recovered from disease symptoms by 21 days after inoculation.
- Taxon
- tomatoSolanum lycopersicumt0
- Material
- Sl-Mae0
- Interactor
- RB-TSWVe0pathogen
- Outcome
- plants recovered from disease symptomse0
- Qualifier
- 21 days post inoculatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In tests with non-grafted plants, Sl-Ma showed resistance to the RB-TSWV infection as viral RNA accumulated at low levels and plants recovered from disease symptoms by 21 days post inoculation.”
t0“Tomato Variety”
Claim · model-written summary
Grafting susceptible tomato genotypes onto Sl-Ma transmitted resistance.
- Taxon
- tomatoSolanum lycopersicume0
- Material
- Sl-Mae0
- Treatment
- grafted onto Sl-Mae0
- Interactor
- RB-TSWVe1pathogen
- Outcome
- resistance traite0
- Direction
- transmittede0
- Comparator
- otherwise highly susceptible tomato genotypese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The resistance trait was transmitted to the otherwise highly susceptible tomato genotypes grafted onto Sl-Ma.”
e1“In tests with non-grafted plants, Sl-Ma showed resistance to the RB-TSWV infection as viral RNA accumulated at low levels and plants recovered from disease symptoms by 21 days post inoculation.”
Claim · model-written summary
RNA silencing was suggested to participate in resistance in Sl-Ma and grafted scions.
- Taxon
- tomatoSolanum lycopersicumt0
- Material
- Sl-Mae0
- Interactor
- RB-TSWVe0pathogen
- Outcome
- resistance showed by Sl-Ma against RB-TSWV and in scions grafted on this rootstocke0
- Qualifier
- RNA silencinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The results from the analysis of small RNAs hallmark genes involved in RNA silencing and virus-induced gene silencing suggest that RNA silencing is involved in the resistance showed by Sl-Ma against RB-TSWV and in scions grafted on this rootstock.”
t0“Tomato Variety”
Claim · model-written summary
Self-grafting susceptible tomato varieties was suggested to enhance RNA silencing.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- self-graftede0
- Outcome
- RNA silencing is enhancede0
- Qualifier
- by the graft itselfe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The results from self-grafted susceptible tomato varieties suggest also that RNA silencing is enhanced by the graft itself.”