Abstract
A putative virus-induced disorder showing mottling and deformation on leaves and fruits of sweet pepper was observed in Taiwan in 2009. Crude sap of symptomatic sweet pepper leaves reacted positively in enzyme-linked immunosorbent assay (ELISA) with antisera to tospoviruses in the Watermelon silver mottle virus (WSMoV) serogroup. A virus culture, TwPep3, isolated from the symptomatic sweet pepper revealed isometric virions measuring about 80–100 nm in diameter via electron microscopy. Seedlings of sweet pepper inoculated with TwPep3 showed similar symptoms as those observed in the field. A 0.9-kb viral genome of isolate TwPep3 could be amplified using degenerate primers for L RNA conserved region of tospoviruses but not using those of tobamoviruses, potyviruses and Cucumber mosaic virus. The partial L RNA sequence of isolate TwPep3 (KF383955) shared 75.1% and 82.4% nucleotide identities with those of WSMoV and Tomato necrotic ringspot virus (TNRV), respectively. To determine the taxonomic relationship of isolate TwPep3, the full length of S RNA (KF383956) was sequenced. Sequence analyses showed that the nucleocapsid (N) gene of TwPep3 shared 82–83% and 87.9–89% nucleotide and amino acid sequence identities, respectively, with those of five isolates of TNRV, the most closely related tospovirus. Moreover, the NSs proteins and S RNAs of TwPep3 and TNRV shared only 78.7% and 77.6% amino acid and nucleotide sequence identities, respectively. Based on our results, isolate TwPep3 should be considered as a new tospovirus and we propose the name Pepper chlorotic spot virus (PCSV). A specific primer pair designed in the N gene was successfully used in reverse transcription-polymerase chain reaction for PCSV diagnostic work.
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Claims 11
Claim · model-written summary
Sweet pepper leaves and fruits showed mottling and deformation in Taiwan.
- Taxon
- sweet pepper
Capsicum annuume0 - Organ / stage
- leaves and fruitse0
- Interactor
- viruse0pathogen
- Outcome
- mottling and deformatione0
- Setting
- Taiwane0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A putative virus-induced disorder showing mottling and deformation on leaves and fruits of sweet pepper was observed in Taiwan in 2009.”
Claim · model-written summary
Symptomatic sweet pepper sap reacted positively with WSMoV serogroup antisera.
- Taxon
- sweet pepperCapsicum annuume0
- Organ / stage
- leavese0
- Interactor
- tospoviruses
Orthotospoviruse0pathogen - Outcome
- reacted positively in enzyme-linked immunosorbent assay (ELISA)e0
- Comparator
- antisera to tospoviruses in the Watermelon silver mottle virus (WSMoV) serogroupe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Crude sap of symptomatic sweet pepper leaves reacted positively in enzyme-linked immunosorbent assay (ELISA) with antisera to tospoviruses in the Watermelon silver mottle virus (WSMoV) serogroup.”
Claim · model-written summary
Inoculated sweet pepper seedlings showed field-like symptoms.
- Taxon
- sweet pepperCapsicum annuume0
- Organ / stage
- Seedlingse0
- Treatment
- inoculated with TwPep3e0
- Interactor
- TwPep3e0pathogen
- Outcome
- similar symptomse0
- Comparator
- those observed in the fielde0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Seedlings of sweet pepper inoculated with TwPep3 showed similar symptoms as those observed in the field.”
Claim · model-written summary
TwPep3 partial L RNA shared 75.1% nucleotide identity with WSMoV.
- Taxon
- sweet pepperCapsicum annuumt0
- Interactor
- TwPep3e0pathogen
- Outcome
- nucleotide identitiese0
- Value
- 75.1%e0
- Comparator
- WSMoVe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The partial L RNA sequence of isolate TwPep3 (KF383955) shared 75.1% and 82.4% nucleotide identities with those of WSMoV and Tomato necrotic ringspot virus (TNRV), respectively.”
t0“sweet pepper”
Claim · model-written summary
TwPep3 partial L RNA shared 82.4% nucleotide identity with TNRV.
- Taxon
- sweet pepperCapsicum annuumt0
- Interactor
- TwPep3e0pathogen
- Outcome
- nucleotide identitiese0
- Value
- 82.4%e0
- Comparator
- Tomato necrotic ringspot virus (TNRV)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The partial L RNA sequence of isolate TwPep3 (KF383955) shared 75.1% and 82.4% nucleotide identities with those of WSMoV and Tomato necrotic ringspot virus (TNRV), respectively.”
t0“sweet pepper”
Claim · model-written summary
TwPep3 N gene shared 82–83% nucleotide identity with TNRV isolates.
- Taxon
- sweet pepperCapsicum annuumt0
- Interactor
- TwPep3e0pathogen
- Gene
- nucleocapsid (N) genee0
- Outcome
- nucleotide and amino acid sequence identitiese0
- Value
- 82–83%e0
- Comparator
- five isolates of TNRVe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sequence analyses showed that the nucleocapsid (N) gene of TwPep3 shared 82–83% and 87.9–89% nucleotide and amino acid sequence identities, respectively, with those of five isolates of TNRV, the most closely related tospovirus.”
t0“sweet pepper”
Claim · model-written summary
TwPep3 N gene shared 87.9–89% amino acid identity with TNRV isolates.
- Taxon
- sweet pepperCapsicum annuumt0
- Interactor
- TwPep3e0pathogen
- Gene
- nucleocapsid (N) genee0
- Outcome
- nucleotide and amino acid sequence identitiese0
- Value
- 87.9–89%e0
- Comparator
- five isolates of TNRVe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sequence analyses showed that the nucleocapsid (N) gene of TwPep3 shared 82–83% and 87.9–89% nucleotide and amino acid sequence identities, respectively, with those of five isolates of TNRV, the most closely related tospovirus.”
t0“sweet pepper”
Claim · model-written summary
TwPep3 and TNRV NSs proteins shared 78.7% amino acid identity.
- Taxon
- sweet pepperCapsicum annuumt0
- Interactor
- TwPep3e0pathogen
- Gene
- NSs proteinse0
- Outcome
- amino acid and nucleotide sequence identitiese0
- Value
- 78.7%e0
- Comparator
- TNRVe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the NSs proteins and S RNAs of TwPep3 and TNRV shared only 78.7% and 77.6% amino acid and nucleotide sequence identities, respectively.”
t0“sweet pepper”
Claim · model-written summary
TwPep3 and TNRV S RNAs shared 77.6% nucleotide identity.
- Taxon
- sweet pepperCapsicum annuumt0
- Interactor
- TwPep3e0pathogen
- Outcome
- amino acid and nucleotide sequence identitiese0
- Value
- 77.6%e0
- Comparator
- TNRVe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the NSs proteins and S RNAs of TwPep3 and TNRV shared only 78.7% and 77.6% amino acid and nucleotide sequence identities, respectively.”
t0“sweet pepper”
Claim · model-written summary
The authors proposed TwPep3 as a new tospovirus named PCSV.
- Taxon
- sweet pepperCapsicum annuumt0
- Interactor
- Pepper chlorotic spot virus (PCSV)Pepper chlorotic spot viruse0pathogen
- Outcome
- new tospoviruse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Based on our results, isolate TwPep3 should be considered as a new tospovirus and we propose the name Pepper chlorotic spot virus (PCSV).”
t0“sweet pepper”
Claim · model-written summary
An N gene primer pair was successful for PCSV diagnosis by RT-PCR.
- Taxon
- sweet pepperCapsicum annuumt0
- Interactor
- PCSVe0pathogen
- Gene
- N genee0
- Outcome
- PCSV diagnostic worke0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A specific primer pair designed in the N gene was successfully used in reverse transcription-polymerase chain reaction for PCSV diagnostic work.”
t0“sweet pepper”
The same questions in other papers
inoculation · disease · Capsicum annuum (sweet pepper)Positive and negative results
11 papers in the same direction as this paper · 7 in the opposite direction
- negative
- positive
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See all 19 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.