Abstract
The wide host range of Ralstonia solanacearum, causal agent of bacterial wilt, and its ability to survive for long periods in the environment restrict the effectiveness of cultural and chemical control measures. The use of phages for disease control is a fast-expanding trend of plant protection with great potential to replace chemical measures. The filamentous phage ϕRSM3 that infects R. solanacearum strains and inactivates virulence on plants is a potential agent for controlling bacterial wilt in tomato. We demonstrated that inoculation of ϕRSM3-infected cells into tomato plants did not cause bacterial wilt. Instead, ϕRSM3-infected cells enhanced the expression of pathogenesis-related (PR) genes, including PR-1a, PR-2b, and PR7, in tomato plants. Moreover, pretreatment with ϕRSM-infected cells protect tomato plants from infection by virulent R. solanacearum strains. The effective dose of ϕRSM3-infected cells for disease prevention was determined to be approximately 10 5 CFU/ml. Because the ϕRSM3-infected cells can grow and continue to produce infectious phage particles under appropriate conditions, ϕRSM phages may serve as an efficient tool to control bacterial wilt in crops.
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Claims 8
Claim · model-written summary
Inoculation of ϕRSM3-infected cells into tomato did not cause bacterial wilt.
- Taxon
- tomato
Solanum lycopersicume0 - Treatment
- inoculation of ϕRSM3-infected cellse0
- Interactor
- ϕRSM3-infected cellse0pathogen
- Outcome
- bacterial wilte0
- Direction
- did not causee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“inoculation of ϕRSM3-infected cells into tomato plants did not cause bacterial wilt”
Claim · model-written summary
ϕRSM3-infected cells enhanced PR-1a expression in tomato.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- ϕRSM3-infected cellse0
- Interactor
- ϕRSM3-infected cellse0pathogen
- Gene
- PR-1ae0
- Outcome
- expression of pathogenesis-related (PR) genese0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ϕRSM3-infected cells enhanced the expression of pathogenesis-related (PR) genes, including PR-1a, PR-2b, and PR7, in tomato plants”
Claim · model-written summary
ϕRSM3-infected cells enhanced PR-2b expression in tomato.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- ϕRSM3-infected cellse0
- Interactor
- ϕRSM3-infected cellse0pathogen
- Gene
- PR-2be0
- Outcome
- expression of pathogenesis-related (PR) genese0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ϕRSM3-infected cells enhanced the expression of pathogenesis-related (PR) genes, including PR-1a, PR-2b, and PR7, in tomato plants”
Claim · model-written summary
ϕRSM3-infected cells enhanced PR7 expression in tomato.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- ϕRSM3-infected cellse0
- Interactor
- ϕRSM3-infected cellse0pathogen
- Gene
- PR7e0
- Outcome
- expression of pathogenesis-related (PR) genese0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ϕRSM3-infected cells enhanced the expression of pathogenesis-related (PR) genes, including PR-1a, PR-2b, and PR7, in tomato plants”
Claim · model-written summary
Pretreatment with ϕRSM-infected cells protected tomato against virulent R. solanacearum.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- pretreatment with ϕRSM-infected cellse0
- Interactor
- R. solanacearum
Ralstonia solanacearume0pathogen - Second interactor
- ϕRSM-infected cellse0biocontrol_agent
- Outcome
- infection by virulent R. solanacearum strainse0
- Direction
- protecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“pretreatment with ϕRSM-infected cells protect tomato plants from infection by virulent R. solanacearum strains”
Claim · model-written summary
The effective disease-prevention dose was approximately 10 5 CFU/ml.
- Taxon
- tomatoSolanum lycopersicume1
- Outcome
- effective dose of ϕRSM3-infected cells for disease preventione0
- Value
- approximately 10 5 CFU/mle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The effective dose of ϕRSM3-infected cells for disease prevention was determined to be approximately 10 5 CFU/ml”
e1“bacterial wilt in tomato”
Claim · model-written summary
ϕRSM3-infected cells can continue to produce infectious phage particles under appropriate conditions.
- Taxon
- tomatoSolanum lycopersicume1
- Outcome
- produce infectious phage particlese0
- Qualifier
- under appropriate conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ϕRSM3-infected cells can grow and continue to produce infectious phage particles under appropriate conditions”
e1“bacterial wilt in tomato”
Claim · model-written summary
The authors propose ϕRSM phages for control of bacterial wilt in crops.
Verbatim
e0“ϕRSM phages may serve as an efficient tool to control bacterial wilt in crops”
The same questions in other papers
inoculation · disease · Solanum lycopersicum (tomato)Positive and negative results
135 papers in the same direction as this paper · 128 in the opposite direction · 2 reporting no difference from an alternative
- negative
- no effect
- negative
262 papers more in this group
See all 266 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.