Abstract
The regulation of excision of Mu elements of the Mutator transposable element family of maize is not well understood. We have used somatic instability of Mu receptor elements from the Bronze 1 and Bronze 2 loci to monitor the frequency and the timing of excision of Mu elements in several tissues. We show that spot size in the aleurone of a bz2::mu1 stock varies between one to approximately 256 cells. This indicates that excision events begin eight divisions prior to full aleurone differentiation and end after the last division of the aleurone. We show that excision is equally biased for late events in all other tissues studied. A locus on chromosome 5 has been identified that affects spot size, possibly by altering the timing of Mu excision. Using somatic excision as an assay of Mutator activity, we found that activity can change in small sectors of the tassel; however, there are no overall activity changes in the tassel during the period of pollen shedding. We also report the recovery of germinal revertants for the bz1::mu1 and bz2::mu1 alleles. One of these revertant alleles was characterized by Southern blot analysis and found to be similar to the progenitor of the mutable allele.
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Claims 8
Claim · model-written summary
In the aleurone of a bz2::mu1 stock, spot size varies from one to about 256 cells in maize.
- Material
- bz2::mu1 stocke1
- Organ / stage
- aleuronee1
- Outcome
- spot sizee1
- Value
- between one to approximately 256 cellse1
Zea maysOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“maize”
e1“spot size in the aleurone of a bz2::mu1 stock varies between one to approximately 256 cells”
Claim · model-written summary
Mu excision events in maize begin eight divisions before full aleurone differentiation and end after the last aleurone division.
- Organ / stage
- aleuronee2
- Outcome
- excision eventse2
- Value
- eight divisions prior to full aleurone differentiatione2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“maize”
e2“This indicates that excision events begin eight divisions prior to full aleurone differentiation and end after the last division of the aleurone”
Claim · model-written summary
Mu excision is equally biased toward late events in all other maize tissues studied.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“maize”
e3“We show that excision is equally biased for late events in all other tissues studied”
Claim · model-written summary
A locus on maize chromosome 5 affects spot size, possibly by altering the timing of Mu excision.
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“maize”
e4“A locus on chromosome 5 has been identified that affects spot size, possibly by altering the timing of Mu excision”
Claim · model-written summary
Mutator activity in maize can change in small sectors of the tassel.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“maize”
e5“we found that activity can change in small sectors of the tassel”
Claim · model-written summary
Maize tassel shows no overall Mutator activity change during the period of pollen shedding.
- Organ / stage
- tassele6
- Outcome
- overall activity changese6
- Qualifier
- during the period of pollen sheddinge6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“maize”
e6“there are no overall activity changes in the tassel during the period of pollen shedding”
Claim · model-written summary
Germinal revertants were recovered for the bz1::mu1 and bz2::mu1 maize alleles.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“maize”
e7“We also report the recovery of germinal revertants for the bz1::mu1 and bz2::mu1 alleles”
Claim · model-written summary
One maize revertant allele was similar to the progenitor of the mutable allele by Southern blot analysis.
- Material
- revertant allelese1
- Outcome
- Southern blot analysise1
- Qualifier
- similar to the progenitor of the mutable allelee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“maize”
e1“One of these revertant alleles was characterized by Southern blot analysis and found to be similar to the progenitor of the mutable allele”