Abstract
Pseudomonas cichorii causes necrotic lesions in eggplant and rot in lettuce. Through transposon insertion into P. cichorii strain SPC9018 we produced two mutants, 4-57 and 2-99, that lost virulence on eggplant but not lettuce. Analyses showed that a transposon was inserted into the hrpG gene in 4-57 and the hrcT gene in 2-99. Nucleotide sequences of the hrp genes of SPC9018 are homologous to those of Pseudomonas viridiflava BS group strains. The pathogenicity of 4-57 on eggplant was restored by transformation with an hrpF operon, originating from either SPC9018 or the BS group member P. viridiflava strain 9504 (Pv9504). These data suggested the involvement of hrp genes in the pathogenicity of SPC9018 on eggplant, and functional conservation of hrpF operons between SPC9018 and Pv9504. Both the hrpS mutant and the hrpL mutant were unable to cause necrotic lesions on eggplant leaves but retained their pathogenicity against lettuce. These results suggest that the pathogenicity of P. cichorii is hrp-dependent in eggplant, but not in lettuce.
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Claims 11
Claim · model-written summary
P. cichorii mutants 4-57 and 2-99 lost virulence on eggplant.
- Taxon
- eggplant
Solanum melongenae0 - Material
- 4-57 and 2-99e0
- Interactor
- Pseudomonas cichorii
.jpg?width=60)
Pseudomonas cichoriie1pathogen - Outcome
- virulence on eggplante0
- Direction
- loste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“two mutants, 4-57 and 2-99, that lost virulence on eggplant but not lettuce”
e1“Pseudomonas cichorii causes necrotic lesions in eggplant and rot in lettuce”
Claim · model-written summary
P. cichorii mutants 4-57 and 2-99 retained virulence on lettuce.
- Taxon
- lettuce
Lactuca sativae1 - Material
- 4-57 and 2-99e0
- Interactor
- Pseudomonas cichoriiPseudomonas cichoriie1pathogen
- Outcome
- virulence on eggplant but not lettucee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two mutants, 4-57 and 2-99, that lost virulence on eggplant but not lettuce”
e1“Pseudomonas cichorii causes necrotic lesions in eggplant and rot in lettuce”
Claim · model-written summary
The 4-57 mutant carried a transposon insertion in hrpG.
- Taxon
- eggplantSolanum melongenat0
- Material
- 4-57e0
- Gene
- hrpGe0
- Outcome
- transposon was inserted into the hrpG genee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a transposon was inserted into the hrpG gene in 4-57”
t0“eggplant but not on lettuce”
Claim · model-written summary
The 2-99 mutant carried a transposon insertion in hrcT.
- Taxon
- eggplantSolanum melongenat0
- Material
- 2-99e0
- Gene
- hrcTe0
- Outcome
- transposon was insertede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the hrcT gene in 2-99”
e1“a transposon was inserted into the hrpG gene in 4-57 and the hrcT gene in 2-99”
t0“eggplant but not on lettuce”
Claim · model-written summary
SPC9018 hrp gene sequences were homologous to those of Pseudomonas viridiflava BS group strains.
- Taxon
- Pseudomonas viridiflava
Pseudomonas viridiflavae0 - Gene
- hrp genese0
- Outcome
- homologous to those of Pseudomonas viridiflava BS group strainse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Nucleotide sequences of the hrp genes of SPC9018 are homologous to those of Pseudomonas viridiflava BS group strains”
t0“eggplant but not on lettuce”
Claim · model-written summary
An hrpF operon from SPC9018 restored eggplant pathogenicity of 4-57.
- Taxon
- eggplantSolanum melongenae0
- Material
- 4-57e0
- Treatment
- transformation with an hrpF operone0
- Interactor
- Pseudomonas cichoriiPseudomonas cichoriie1pathogen
- Gene
- hrpF operone0
- Outcome
- pathogenicity of 4-57 on eggplante0
- Direction
- restorede0
- Qualifier
- SPC9018e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The pathogenicity of 4-57 on eggplant was restored by transformation with an hrpF operon, originating from either SPC9018 or the BS group member P. viridiflava strain 9504 (Pv9504)”
e1“Pseudomonas cichorii causes necrotic lesions in eggplant”
Claim · model-written summary
An hrpF operon from P. viridiflava strain 9504 restored eggplant pathogenicity of 4-57.
- Taxon
- eggplantSolanum melongenae0
- Material
- 4-57e0
- Treatment
- transformation with an hrpF operone0
- Interactor
- Pseudomonas cichoriiPseudomonas cichoriie1pathogen
- Gene
- hrpF operone0
- Outcome
- pathogenicity of 4-57 on eggplante0
- Direction
- restorede0
- Qualifier
- P. viridiflava strain 9504 (Pv9504)e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The pathogenicity of 4-57 on eggplant was restored by transformation with an hrpF operon, originating from either SPC9018 or the BS group member P. viridiflava strain 9504 (Pv9504)”
e1“Pseudomonas cichorii causes necrotic lesions in eggplant”
Claim · model-written summary
The hrpS mutant could not cause necrotic lesions on eggplant leaves.
- Taxon
- eggplantSolanum melongenae0
- Material
- hrpS mutante0
- Organ / stage
- leavese0
- Interactor
- Pseudomonas cichoriiPseudomonas cichoriie1pathogen
- Gene
- hrpSe0
- Outcome
- unable to cause necrotic lesions on eggplant leavese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both the hrpS mutant and the hrpL mutant were unable to cause necrotic lesions on eggplant leaves but retained their pathogenicity against lettuce”
e1“Pseudomonas cichorii causes necrotic lesions in eggplant”
Claim · model-written summary
The hrpL mutant could not cause necrotic lesions on eggplant leaves.
- Taxon
- eggplantSolanum melongenae0
- Material
- hrpL mutante0
- Organ / stage
- leavese0
- Interactor
- Pseudomonas cichoriiPseudomonas cichoriie1pathogen
- Gene
- hrpLe0
- Outcome
- unable to cause necrotic lesions on eggplant leavese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both the hrpS mutant and the hrpL mutant were unable to cause necrotic lesions on eggplant leaves but retained their pathogenicity against lettuce”
e1“Pseudomonas cichorii causes necrotic lesions in eggplant”
Claim · model-written summary
The hrpS mutant retained pathogenicity against lettuce.
- Taxon
- lettuceLactuca sativae0
- Material
- hrpS mutante0
- Interactor
- Pseudomonas cichoriiPseudomonas cichoriie1pathogen
- Gene
- hrpSe0
- Outcome
- retained their pathogenicity against lettucee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both the hrpS mutant and the hrpL mutant were unable to cause necrotic lesions on eggplant leaves but retained their pathogenicity against lettuce”
e1“Pseudomonas cichorii causes necrotic lesions in eggplant and rot in lettuce”
Claim · model-written summary
The hrpL mutant retained pathogenicity against lettuce.
- Taxon
- lettuceLactuca sativae0
- Material
- hrpL mutante0
- Interactor
- Pseudomonas cichoriiPseudomonas cichoriie1pathogen
- Gene
- hrpLe0
- Outcome
- retained their pathogenicity against lettucee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both the hrpS mutant and the hrpL mutant were unable to cause necrotic lesions on eggplant leaves but retained their pathogenicity against lettuce”
e1“Pseudomonas cichorii causes necrotic lesions in eggplant and rot in lettuce”