Abstract
Application of anther culture techniques for improvement of indica rices is a formidable task as they are known to be recalcitrant to culture unlike japonica rices. Since anther culture can effectively address the major problems associated with the adoption of hybrid rice in India, an effort was made to assess the influence of cold pretreatment and phyto hormones on the anther culture response of Rajalaxmi (CRHR 5) and Ajay (CRHR 7), two elite and popular indica rice hybrids. Cold pretreatment for 7-9 days at 10 o C was found to have a positive influence on the callus induction frequency irrespective of the media employed and prolonged treatment over the optimum proved to be inhibitory. Of the different auxins (2, 4-D, NAA) and cytokinins (Kinetin, BAP) and their combinations studied, a ratio of 1:4 for 2,4-D and NAA and 1:3:1 ratio of Kinetin: BAP: NAA ratio proved to be optimal for callus induction and green plant regeneration respectively. This information can be of use in the development of doubled haploids with superior yield and good grain quality from the elite hybrid cultivars.
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Claims 4
Claim · model-written summary
Cold pretreatment for 7-9 days at 10 °C had a positive influence on callus induction frequency of indica rice in anther culture.
- Taxon
- indica rice
Oryza sativa Indica Groupe2 - Treatment
- Cold pretreatment for 7-9 days at 10 o Ce1
- Outcome
- callus induction frequencye1
- Direction
- positive influencee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Cold pretreatment for 7-9 days at 10 o C was found to have a positive influence on the callus induction frequency irrespective of the media employed and prolonged treatment over the optimum proved to be inhibitory”
e2“two elite and popular indica rice hybrids”
Claim · model-written summary
Prolonged cold treatment beyond the optimum was inhibitory to callus induction frequency of indica rice.
- Taxon
- indica riceOryza sativa Indica Groupe2
- Treatment
- prolonged treatment over the optimume1
- Outcome
- callus induction frequencye1
- Direction
- inhibitorye1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Cold pretreatment for 7-9 days at 10 o C was found to have a positive influence on the callus induction frequency irrespective of the media employed and prolonged treatment over the optimum proved to be inhibitory”
e2“two elite and popular indica rice hybrids”
Claim · model-written summary
A 1:4 ratio of 2,4-D and NAA was optimal for callus induction in indica rice anther culture.
- Taxon
- indica riceOryza sativa Indica Groupe2
- Treatment
- a ratio of 1:4 for 2,4-D and NAAe1
- Outcome
- callus inductione1
- Direction
- optimale1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“a ratio of 1:4 for 2,4-D and NAA and 1:3:1 ratio of Kinetin: BAP: NAA ratio proved to be optimal for callus induction and green plant regeneration respectively”
e2“two elite and popular indica rice hybrids”
Claim · model-written summary
A 1:3:1 ratio of Kinetin: BAP: NAA was optimal for green plant regeneration in indica rice anther culture.
- Taxon
- indica riceOryza sativa Indica Groupe2
- Treatment
- 1:3:1 ratio of Kinetin: BAP: NAAe1
- Outcome
- green plant regeneratione1
- Direction
- optimale1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“a ratio of 1:4 for 2,4-D and NAA and 1:3:1 ratio of Kinetin: BAP: NAA ratio proved to be optimal for callus induction and green plant regeneration respectively”
e2“two elite and popular indica rice hybrids”
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