Abstract
Improvement of indica rice lines is a challenging task through application of anther culture as they are recalcitrant to culture distinctive to japonica rices. Current investigation aimed to verify the effect of cold pretreatment and phytohormones on the anther culture response of 6 crosses. Cold pretreatment for 7-10 days at 100C was found to have profound effect on callus induction frequency irrespective of the media employed and the prolonged treatment over the optimum showed to be inhibitory. Of the different auxins (2,4-D, NAA) and cytokinins (Kinetin, BAP)and their combinations studied, a ratio of 1:4 for 2,4-D and NAA and 1:3:1 ratio of Kinetin: BAP: NAA ratio proved to be optimal for callus induction and green plant regeneration respectively. This information suggests the opportunity of exploiting hybrid vigor in indica rice cultivars for the development of doubled haploids with more yield and yield attributes from elite hybrid crosses.
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Claims 4
Claim · model-written summary
Cold pretreatment for 7–10 days enhanced callus induction.
- Taxon
- Rice
Oryza sativat0 - Treatment
- Cold pretreatment for 7-10 days at 100Ce0
- Outcome
- callus induction frequencye0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cold pretreatment for 7-10 days at 100C was found to have profound effect on callus induction frequency irrespective of the media employed and the prolonged treatment over the optimum showed to be inhibitory.”
t0“Rice”
Claim · model-written summary
Pretreatment longer than the optimum inhibited callus induction.
- Taxon
- RiceOryza sativat0
- Treatment
- prolonged treatment over the optimume0
- Outcome
- callus induction frequencye1
- Direction
- inhibitorye0
- Comparator
- the optimume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the prolonged treatment over the optimum showed to be inhibitory.”
e1“callus induction frequency”
t0“Rice”
Claim · model-written summary
A 1:4 ratio of 2,4-D and NAA was optimal for callus induction.
- Taxon
- RiceOryza sativat0
- Treatment
- a ratio of 1:4 for 2,4-D and NAAe0
- Outcome
- callus inductione0
- Direction
- optimale0
- Value
- 1:4e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a ratio of 1:4 for 2,4-D and NAA and 1:3:1 ratio of Kinetin: BAP: NAA ratio proved to be optimal for callus induction and green plant regeneration respectively.”
t0“Rice”
Claim · model-written summary
A 1:3:1 ratio of Kinetin, BAP and NAA was optimal for green plant regeneration.
- Taxon
- RiceOryza sativat0
- Treatment
- 1:3:1 ratio of Kinetin: BAP: NAAe0
- Outcome
- green plant regeneratione0
- Direction
- optimale0
- Value
- 1:3:1e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“1:3:1 ratio of Kinetin: BAP: NAA ratio proved to be optimal for callus induction and green plant regeneration respectively.”
t0“Rice”
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