Abstract
The Malnad region located in the Western Ghats of Karnataka is known for the cultivation of indigenous rain fed land race cultivar of rice. The present study was to investigate the callogenic and caulogenic potentialities of the two indigenous rice cultivar namely Karimundaga and Kanadatumba using dehusked mature embryo explants. For callus and shoot bud differentiation, the explants were cultured on Murashige and Skoog (MS) medium supplemented with 2,4-D (1-3 mg/L), IAA (1-2 mg/L), Kn (1-4 mg/L) and BAP (1-4 mg/L). The morphogenic potentialities of the two rice cultivar differed in texture of callus. In both the cultivar callogenic frequency was optimized at 1 mg/L 2,4-D concentration, it was 94% in Karimundaga and 58% in Kanadatumba. Supplementation of IAA either alone (1-2 mg/L) or in combination with Kn or BAP at 1 to 4 mg/L concentration of each induces shoot bud differentiation from the calli. In the cultivar Karimundaga caulogenic frequency was highest (10.60 +/- 2.55) at 1.0 mg/L IAA and 4.0 mg/L BAP concentration. While in the cultivar Kanadatumba highest number of shoot buds (7.90 +/- 2.69) was differentiated at 1.0 mg/L IAA and 4.0 mg/L Kn concentration. The calli derived regenerants were successfully acclimatized in the greenhouse and agro-morphological variations were evaluated. The growth characteristics and yield related parameters exhibited by in vitro plants were lower than the in vivo plants.
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Claims 6
Claim · model-written summary
In Karimundaga, callogenic frequency was optimized at 1 mg/L 2,4-D, at 94%.
- Taxon
- rice
Oryza sativat0 - Material
- Karimundagae1
- Organ / stage
- dehusked mature embryo explantse0
- Treatment
- 1 mg/L 2,4-De1
- Outcome
- callogenic frequencye1
- Direction
- optimizede1
- Value
- 94%e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Regeneration of plantlets from mature embryo calli of Western Ghats land race cultivar of rice, Oryza sativa L.”
e0“using dehusked mature embryo explants”
e1“In both the cultivar callogenic frequency was optimized at 1 mg/L 2,4-D concentration, it was 94% in Karimundaga and 58% in Kanadatumba”
Claim · model-written summary
In Kanadatumba, callogenic frequency was optimized at 1 mg/L 2,4-D, at 58%.
- Taxon
- riceOryza sativat0
- Material
- Kanadatumbae1
- Organ / stage
- dehusked mature embryo explantse0
- Treatment
- 1 mg/L 2,4-De1
- Outcome
- callogenic frequencye1
- Direction
- optimizede1
- Value
- 58%e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Regeneration of plantlets from mature embryo calli of Western Ghats land race cultivar of rice, Oryza sativa L.”
e0“using dehusked mature embryo explants”
e1“In both the cultivar callogenic frequency was optimized at 1 mg/L 2,4-D concentration, it was 94% in Karimundaga and 58% in Kanadatumba”
Claim · model-written summary
IAA alone or combined with Kn or BAP at 1 to 4 mg/L induces shoot bud differentiation from the calli.
- Taxon
- riceOryza sativat0
- Treatment
- IAA either alone (1-2 mg/L) or in combination with Kn or BAP at 1 to 4 mg/Le4
- Outcome
- shoot bud differentiation from the callie4
- Direction
- inducese4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Regeneration of plantlets from mature embryo calli of Western Ghats land race cultivar of rice, Oryza sativa L.”
e4“Supplementation of IAA either alone (1-2 mg/L) or in combination with Kn or BAP at 1 to 4 mg/L concentration of each induces shoot bud differentiation from the calli”
Claim · model-written summary
In Karimundaga, caulogenic frequency was highest (10.60 +/- 2.55) at 1.0 mg/L IAA and 4.0 mg/L BAP.
- Taxon
- riceOryza sativat0
- Material
- Karimundagae2
- Treatment
- 1.0 mg/L IAA and 4.0 mg/L BAPe2
- Outcome
- caulogenic frequencye2
- Direction
- higheste2
- Value
- 10.60 +/- 2.55e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Regeneration of plantlets from mature embryo calli of Western Ghats land race cultivar of rice, Oryza sativa L.”
e2“In the cultivar Karimundaga caulogenic frequency was highest (10.60 +/- 2.55) at 1.0 mg/L IAA and 4.0 mg/L BAP concentration”
Claim · model-written summary
In Kanadatumba, the highest number of shoot buds (7.90 +/- 2.69) was obtained at 1.0 mg/L IAA and 4.0 mg/L Kn.
- Taxon
- riceOryza sativat0
- Material
- Kanadatumbae3
- Treatment
- 1.0 mg/L IAA and 4.0 mg/L Kne3
- Outcome
- number of shoot budse3
- Direction
- higheste3
- Value
- 7.90 +/- 2.69e3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Regeneration of plantlets from mature embryo calli of Western Ghats land race cultivar of rice, Oryza sativa L.”
e3“While in the cultivar Kanadatumba highest number of shoot buds (7.90 +/- 2.69) was differentiated at 1.0 mg/L IAA and 4.0 mg/L Kn concentration”
Claim · model-written summary
Regenerated in vitro plants had lower growth and yield-related parameters than in vivo plants.
- Taxon
- riceOryza sativat0
- Treatment
- in vitroe5
- Outcome
- growth characteristics and yield related parameterse5
- Direction
- lowere5
- Comparator
- in vivo plantse5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Regeneration of plantlets from mature embryo calli of Western Ghats land race cultivar of rice, Oryza sativa L.”
e5“The growth characteristics and yield related parameters exhibited by in vitro plants were lower than the in vivo plants”
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in vitro · yield · Oryza sativa (rice)Positive and negative results
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