Abstract
Rapeseed (Brassica napus L.) is one of the most important oil crops worldwide and has a significant potential for cultivation in Lithuania. One of the most important agronomic traits in winter rapeseed (Brassica napus L.) is winter-hardiness or freezing tolerance, which can be enhanced by various exogenous factors. Investigations were carried out in the laboratory of Agrobiotechnology of Aleksandras Stulginskis University. Changes in proline and soluble sugars levels in response to cold de-acclimation and exogenous amino acids were monitored in vitro in winter rapeseed shoots. Shoots of variety ‘Sunday’ were acclimated at 4°C for 14 days and de-acclimated under temperatures of 18/16oC, photoperiod 16/8 h (day/night) for 1, 3, 5 and 7 days. Exposure of cold-acclimated shoots to normal growth temperature significantly reduced cold tolerance as well as soluble sugars and proline contents. Exogenous amino acids caused significant increase in proline contents. The membrane injury for leaf discs under freezing stress was estimated by the electrolyte leakage test. The degree of cold tolerance of the shoots from the different treatment was substantially decreased with increasing de-acclimation duration. The addition of exogenous amino acids to the culture medium resulted in significant decrease in electrolyte leakage. On the medium supplemented with exogenous amino acids after de-acclimation treatment electrolyte leakage of proline-treated shoots was from 6.97 to 11.93% lower in comparison with non-treated shoots.
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Claims 5
Claim · model-written summary
Exposing cold-acclimated rapeseed shoots to normal growth temperature significantly reduced cold tolerance, soluble sugars and proline contents.
- Taxon
- rapeseedt0
- Treatment
- Exposure of cold-acclimated shoots to normal growth temperaturee0
- Outcome
- cold tolerance as well as soluble sugars and proline contentse0
- Direction
- reducede0
Not reportedComparator
Verbatim
t0“rapeseed”
e0“Exposure of cold-acclimated shoots to normal growth temperature significantly reduced cold tolerance as well as soluble sugars and proline contents”
Claim · model-written summary
Exogenous amino acids significantly increased proline contents in rapeseed shoots.
Not reportedComparator
Verbatim
t0“rapeseed”
e0“Exogenous amino acids caused significant increase in proline contents”
Claim · model-written summary
The degree of cold tolerance of rapeseed shoots decreased substantially with increasing de-acclimation duration.
- Taxon
- rapeseedt0
- Treatment
- increasing de-acclimation duratione0
- Outcome
- degree of cold tolerancee0
- Direction
- decreasede0
Not reportedComparator
Verbatim
t0“rapeseed”
e0“The degree of cold tolerance of the shoots from the different treatment was substantially decreased with increasing de-acclimation duration”
Claim · model-written summary
Adding exogenous amino acids to the culture medium significantly decreased electrolyte leakage in rapeseed shoots.
- Taxon
- rapeseedt0
- Treatment
- exogenous amino acids to the culture mediume0
- Outcome
- electrolyte leakagee0
- Direction
- decreasee0
Not reportedComparator
Verbatim
t0“rapeseed”
e0“The addition of exogenous amino acids to the culture medium resulted in significant decrease in electrolyte leakage”
Claim · model-written summary
Electrolyte leakage of proline-treated rapeseed shoots was 6.97 to 11.93% lower than that of non-treated shoots.
Verbatim
t0“rapeseed”
e0“electrolyte leakage of proline-treated shoots was from 6.97 to 11.93% lower in comparison with non-treated shoots”
The same questions in other papers
in vitro · physiology: oxidative · Brassica napus (oilseed rape)Positive and negative results
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cold · physiology: osmolytes · Brassica napus (oilseed rape)Positive and negative results
2 papers in the same direction as this paper · 4 in the opposite direction
- positive
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See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.