Abstract
A comparison of the sequences in an internally transcribed spacer (ITS) 1 region of rDNA between clonally regenerated A.vera and the same species in Japan, USA and Egypt revealed the presence of two types of nucleotide sequences, 252 and 254 bps. Based on the findings in the ITS 1 region, A.vera having 252 and 254 bps clearly showed a stable sequence similarity, suggesting high conservation of the base peak sequence in the ITS 1 region. However, frequent base substitutions in the 252 bps sample leaves that came from callus tissue and micropropagated plants were observed around the regions of nucleotide positions 66, 99 and 199-201.The minor deviation in clonally regenerated A.vera may be due to the stage of regeneration and cell specification in cases of the callus tissue. In the present study, the base peak sequence of the ITS 1 region of rDNA was adopted as a molecular marker for differentiating A.vera plants from geographically distributed and clonally regenerated A.vera plants, and it was suggested that the base peak substitutions in the ITS 1 region may arise from the different nutritional and environmental factors in cultivation and plant growth stages. אא F
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Claims 6
Claim · model-written summary
Aloe vera plants showed two rDNA ITS1 sequence types, of 252 and 254 bp.
Verbatim
e1“clonally regenerated A.vera and the same species in Japan, USA and Egypt revealed the presence of two types of nucleotide sequences, 252 and 254 bps”
Claim · model-written summary
Aloe vera with 252 and 254 bp ITS1 sequences showed a stable sequence similarity, suggesting high conservation of the ITS1 base peak sequence.
Verbatim
e2“Based on the findings in the ITS 1 region, A.vera having 252 and 254 bps clearly showed a stable sequence similarity, suggesting high conservation of the base peak sequence in the ITS 1 region”
Claim · model-written summary
Leaves of the 252 bp sample from callus tissue and micropropagated plants showed frequent base substitutions around nucleotide positions 66, 99 and 199-201.
Verbatim
e1“clonally regenerated A.vera and the same species in Japan, USA and Egypt revealed the presence of two types of nucleotide sequences, 252 and 254 bps”
e3“frequent base substitutions in the 252 bps sample leaves that came from callus tissue and micropropagated plants were observed around the regions of nucleotide positions 66, 99 and 199-201”
Claim · model-written summary
The minor deviation in clonally regenerated Aloe vera may be due to the stage of regeneration and cell specification in callus.
Verbatim
e4“The minor deviation in clonally regenerated A.vera may be due to the stage of regeneration and cell specification in cases of the callus tissue”
Claim · model-written summary
Base peak substitutions in the ITS1 region of Aloe vera may arise from different nutritional and environmental factors in cultivation and growth stages.
Verbatim
e1“clonally regenerated A.vera and the same species in Japan, USA and Egypt revealed the presence of two types of nucleotide sequences, 252 and 254 bps”
e5“it was suggested that the base peak substitutions in the ITS 1 region may arise from the different nutritional and environmental factors in cultivation and plant growth stages”
Claim · model-written summary
The ITS1 base peak sequence was adopted as a molecular marker to differentiate Aloe vera plants from geographically distributed and clonally regenerated plants.
Verbatim
e6“the base peak sequence of the ITS 1 region of rDNA was adopted as a molecular marker for differentiating A.vera plants from geographically distributed and clonally regenerated A.vera plants”