Abstract
Common bean is one of the most recalcitrant species for in vitro manipulation and transformation. In this study efficient genetic transformation methods were developed and performed in commercialized bean varieties calli by utilized microparticle bombardment (direct biolistic gun) and indirect A. tumefaciens-based technique. Hypocotyl segments cultured on Gamborgs medium supplemented with 1 mg l-l kinetin and 2 mg l-l 2,4-dichlorophenoxy acetic acid proved the best for callus induction and maintenance. Co-transformed calli were double selected on selective medium using 150 mg l-l kanamycin and 0.8 mol l-l mannitol. Transient genes expressions were obtained in calli cells and the results showed that the cells shot by biolistic DNA delivery method can be transformed to get kanamycin resistant and mannitol tolerant. The presence and integration of the neomycin phosphotransferase II (npt II) and osmosis protector mannitol-1-phosphate dehydrogenase (mtlD) genes into P. vulgaris L. genome were confirmed and verified by double selection tests. Transgenic calli were selected after co-cultivated with Agrobacterium method on selective medium containing 5 mg l-l phosphinotricin (PPT). Transient genes expressions were obtained in the calli and the results showed that the common beans calli co-cultivated with Agrobacterium can be transformed to get phosphinotricin resistant and β-glucuronidase reporter positive tissues. The presence and integration of the phosphinotricin acetyl transferase and β-glucuronidase genes into P. vulgaris L. genome were confirmed and verified by marker selection and histochemical assay tests. These results suggest an efficient biolistic gun, and Agrobacterium-mediated transformation methods for stable integration of economically important genes into common beans calli, and that these transformations systems could be useful for future studies on transferring economically important genes into common bean plants.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 6
Claim · model-written summary
Hypocotyl segments on supplemented Gamborgs medium were best for callus induction and maintenance.
- Taxon
- COMMON BEANSt0
- Organ / stage
- Hypocotyl segmentse0
- Treatment
- Gamborgs medium supplemented with 1 mg l-l kinetin and 2 mg l-l 2,4-dichlorophenoxy acetic acide0
- Outcome
- callus induction and maintenancee0
- Direction
- beste0
Not reportedComparator
Verbatim
e0“Hypocotyl segments cultured on Gamborgs medium supplemented with 1 mg l-l kinetin and 2 mg l-l 2,4-dichlorophenoxy acetic acid proved the best for callus induction and maintenance.”
t0“COMMON BEANS (Phaseolus vulgaris L.)”
Claim · model-written summary
Biolistic DNA delivery produced kanamycin-resistant and mannitol-tolerant calli.
- Taxon
- COMMON BEANSt0
- Treatment
- biolistic DNA delivery methode0
- Outcome
- kanamycin resistant and mannitol tolerante0
Not reportedComparatorValue
Verbatim
e0“the cells shot by biolistic DNA delivery method can be transformed to get kanamycin resistant and mannitol tolerant”
t0“COMMON BEANS (Phaseolus vulgaris L.)”
Claim · model-written summary
Double selection confirmed integration of npt II and mtlD genes into the P. vulgaris genome.
- Taxon
- P. vulgaris L.
Phaseolus vulgarise0 - Gene
- neomycin phosphotransferase II (npt II) and osmosis protector mannitol-1-phosphate dehydrogenase (mtlD) genese0
- Outcome
- presence and integratione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The presence and integration of the neomycin phosphotransferase II (npt II) and osmosis protector mannitol-1-phosphate dehydrogenase (mtlD) genes into P. vulgaris L. genome were confirmed and verified by double selection tests.”
Claim · model-written summary
Agrobacterium co-cultivation produced phosphinotricin-resistant and β-glucuronidase-positive common bean calli.
- Taxon
- common beansPhaseolus vulgarise0
- Treatment
- co-cultivated with Agrobacteriume0
- Outcome
- phosphinotricin resistant and β-glucuronidase reporter positive tissuese0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the common beans calli co-cultivated with Agrobacterium can be transformed to get phosphinotricin resistant and β-glucuronidase reporter positive tissues.”
Claim · model-written summary
Marker selection and histochemical assays confirmed integration of phosphinotricin acetyl transferase and β-glucuronidase genes into P. vulgaris.
- Taxon
- P. vulgaris L.Phaseolus vulgarise0
- Gene
- phosphinotricin acetyl transferase and β-glucuronidase genese0
- Outcome
- presence and integratione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The presence and integration of the phosphinotricin acetyl transferase and β-glucuronidase genes into P. vulgaris L. genome were confirmed and verified by marker selection and histochemical assay tests.”
Claim · model-written summary
The authors suggest both transformation systems could transfer economically important genes into common bean plants.
- Taxon
- common beanPhaseolus vulgarise0
- Outcome
- transferring economically important genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“these transformations systems could be useful for future studies on transferring economically important genes into common bean plants.”
The same questions in other papers
acetic acid · growth · Phaseolus vulgaris (common bean)Positive and negative results
1 in the opposite direction
- negative
kinetin · growth · Phaseolus vulgaris (common bean)Positive and negative results
5 papers in the same direction as this paper · 3 in the opposite direction
- negative
- negative
- no effect
5 papers more in this group
See all 9 papers →in vitro · growth · Phaseolus vulgaris (common bean)Positive and negative results
10 papers in the same direction as this paper · 7 in the opposite direction
- negative
- negative
- negative
14 papers more in this group
See all 18 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.