Abstract
LI LM, OUYANG LJ & GAN SM. 2015. Towards an efficient regeneration protocol for Eucalyptus urophylla. The aim of the present study was to establish an efficient regeneration system for Eucalyptus urophylla by means of organogenesis. The hypocotyls from seedlings of E. urophylla clone U6 were used as explants and cultured in a modified Murashige and Skoog (MS) medium, supplemented with 13.2 μM L-1 N-phenylN’-[6-(2-chlorobenzothiazol)-yl] urea (PBU) and 0.285μM L-1 indole-3-acetic acid (IAA). After culture for 5 days, 98.8% explants formed callus. After 30 days, the calli obtained were transferred to Schwarz differential medium (SDM) containing different combinations of 6-benzyladenine (6-BA) and naphthalene acetic acid (NAA). Compared with other growth regulator combinations, PBU stimulated more vigorous calli and restrained browning. In addition, a large percentage (58.6%) of the calli induced by PBU showed adventitious bud formation. Proliferation of adventitious buds was obtained on SDM medium supplemented with 2.2 μM L-1 6-BA and 0.25 μM L-1 NAA and shoot elongation was then stimulated on SDM medium supplemented with 5.28 μM L-1PBU and 0.25 μM L-1 NAA for 20 days. For rooting, the elongated shoots were cultivated on root induction medium containing 2.46 μM L-1 indole-3-butyric acid (IBA). Plantlets were then successfully transplanted to a greenhouse. This paper presents an efficient procedure for regeneration of E. urophylla.
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Claims 8
Claim · model-written summary
The specified medium produced callus on 98.8% of explants after 5 days.
- Taxon
- E. urophylla
Eucalyptus urophyllae0 - Material
- clone U6e0
- Organ / stage
- hypocotylse0
- Treatment
- 13.2 μM L-1 N-phenylN’-[6-(2-chlorobenzothiazol)-yl] urea (PBU) and 0.285μM L-1 indole-3-acetic acid (IAA)e0
- Outcome
- explants formed calluse0
- Value
- 98.8%e0
- Qualifier
- After culture for 5 dayse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The hypocotyls from seedlings of E. urophylla clone U6 were used as explants and cultured in a modified Murashige and Skoog (MS) medium, supplemented with 13.2 μM L-1 N-phenylN’-[6-(2-chlorobenzothiazol)-yl] urea (PBU) and 0.285μM L-1 indole-3-acetic acid (IAA). After culture for 5 days, 98.8% explants formed callus.”
Claim · model-written summary
PBU stimulated more vigorous callus than other growth regulator combinations.
- Taxon
- Eucalyptus urophyllaEucalyptus urophyllat0
- Treatment
- PBUe0
- Outcome
- vigorous callie0
- Direction
- moree0
- Comparator
- other growth regulator combinationse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Compared with other growth regulator combinations, PBU stimulated more vigorous calli and restrained browning.”
t0“Eucalyptus urophylla”
Claim · model-written summary
PBU restrained callus browning relative to other growth regulator combinations.
- Taxon
- Eucalyptus urophyllaEucalyptus urophyllat0
- Treatment
- PBUe0
- Outcome
- browninge0
- Direction
- restrainede0
- Comparator
- other growth regulator combinationse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Compared with other growth regulator combinations, PBU stimulated more vigorous calli and restrained browning.”
t0“Eucalyptus urophylla”
Claim · model-written summary
PBU-induced calli formed adventitious buds at 58.6%.
- Taxon
- Eucalyptus urophyllaEucalyptus urophyllat0
- Treatment
- PBUe0
- Outcome
- adventitious bud formatione0
- Value
- 58.6%e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a large percentage (58.6%) of the calli induced by PBU showed adventitious bud formation.”
t0“Eucalyptus urophylla”
Claim · model-written summary
SDM with 2.2 μM L-1 6-BA and 0.25 μM L-1 NAA supported adventitious bud proliferation.
- Taxon
- Eucalyptus urophyllaEucalyptus urophyllat0
- Treatment
- SDM medium supplemented with 2.2 μM L-1 6-BA and 0.25 μM L-1 NAAe0
- Outcome
- Proliferation of adventitious budse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Proliferation of adventitious buds was obtained on SDM medium supplemented with 2.2 μM L-1 6-BA and 0.25 μM L-1 NAA”
t0“Eucalyptus urophylla”
Claim · model-written summary
SDM with 5.28 μM L-1 PBU and 0.25 μM L-1 NAA stimulated shoot elongation over 20 days.
- Taxon
- Eucalyptus urophyllaEucalyptus urophyllat0
- Treatment
- SDM medium supplemented with 5.28 μM L-1PBU and 0.25 μM L-1 NAAe0
- Outcome
- shoot elongatione0
- Direction
- stimulatede0
- Qualifier
- for 20 dayse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“shoot elongation was then stimulated on SDM medium supplemented with 5.28 μM L-1PBU and 0.25 μM L-1 NAA for 20 days.”
t0“Eucalyptus urophylla”
Claim · model-written summary
Root induction medium containing 2.46 μM L-1 IBA supported rooting of elongated shoots.
- Taxon
- Eucalyptus urophyllaEucalyptus urophyllat0
- Treatment
- root induction medium containing 2.46 μM L-1 indole-3-butyric acid (IBA)e0
- Outcome
- rootinge0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“For rooting, the elongated shoots were cultivated on root induction medium containing 2.46 μM L-1 indole-3-butyric acid (IBA).”
t0“Eucalyptus urophylla”
Claim · model-written summary
Regenerated plantlets were successfully transplanted to a greenhouse.
- Taxon
- Eucalyptus urophyllaEucalyptus urophyllat0
- Outcome
- successfully transplantede0
- Setting
- greenhousee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Plantlets were then successfully transplanted to a greenhouse.”
t0“Eucalyptus urophylla”
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