Abstract
Locating of important agronomic genes onto chromosome is helpful for efficient development of new wheat varieties. Wheat chromosome substitution lines between two varieties have been widely used for locating genes because of their distinctive advantages in genetic analysis, compared with the aneuploid genetic materials. Apart from the substituted chromosome, the other chromosomes between the substitution lines and their recipient parent should be identical, which eases the gene locating practice. In this study, a set of chromosome substitution lines with cv. Wichita (WI) as the recipient parent and cv. Cheyenne (CNN) as the donor parent were studied for the composition of high molecular weight glutenin subunits (HMW-GS) as well as a range of agronomic important traits. Results revealed that the substitution lines of WI(CNN5D), WI(CNN6A) and WI(CNN7B) had higher plant heights than the two parents of WI and CNN, and WI(CNN3D) had later maturity than the parents. By sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) analysis, a substitution line WI(CNN5B) was found to contain different HMW-GS patterns from its two parents, in which 1By9 was replaced by 1By8 on chromosome 1BL. Simple sequence repeat (SSR) analysis confirmed that the variation on 1BL in WI(CNN5B) was originated from Chinese Spring (CS). It is concluded that chromosomal fragments from bridge material and donor parent were quite often retained in intracultivaral chromosome substitution lines except the substituting chromosomes.
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Claims 6
Claim · model-written summary
WI(CNN5D), WI(CNN6A), and WI(CNN7B) had greater plant height than both parents.
- Taxon
- common wheat
Triticum aestivumt0 - Material
- WI(CNN5D), WI(CNN6A) and WI(CNN7B)e0
- Outcome
- plant heightse0
- Direction
- highere0
- Comparator
- the two parents of WI and CNNe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the substitution lines of WI(CNN5D), WI(CNN6A) and WI(CNN7B) had higher plant heights than the two parents of WI and CNN”
t0“common wheat”
Claim · model-written summary
WI(CNN3D) matured later than its parents.
- Taxon
- common wheatTriticum aestivumt0
- Material
- WI(CNN3D)e0
- Outcome
- maturitye0
- Direction
- latere0
- Comparator
- the parentse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“WI(CNN3D) had later maturity than the parents”
t0“common wheat”
Claim · model-written summary
WI(CNN5B) had a different HMW-GS pattern from both parents.
- Taxon
- common wheatTriticum aestivumt0
- Material
- WI(CNN5B)e0
- Gene
- HMW-GSe0
- Outcome
- different HMW-GS patternse0
- Comparator
- its two parentse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a substitution line WI(CNN5B) was found to contain different HMW-GS patterns from its two parents”
t0“common wheat”
Claim · model-written summary
In WI(CNN5B), 1By9 was replaced by 1By8 on chromosome 1BL.
- Taxon
- common wheatTriticum aestivumt0
- Material
- WI(CNN5B)e1
- Gene
- 1By9e0
- Outcome
- replaced by 1By8 on chromosome 1BLe0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in which 1By9 was replaced by 1By8 on chromosome 1BL”
e1“WI(CNN5B) was found to contain different HMW-GS patterns”
t0“common wheat”
Claim · model-written summary
SSR analysis traced the WI(CNN5B) chromosome 1BL variation to Chinese Spring.
- Taxon
- common wheatTriticum aestivumt0
- Material
- WI(CNN5B)e0
- Gene
- 1BLe0
- Outcome
- originated from Chinese Spring (CS)e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the variation on 1BL in WI(CNN5B) was originated from Chinese Spring (CS)”
t0“common wheat”
Claim · model-written summary
Chromosomal fragments from bridge material and the donor parent were often retained outside substituted chromosomes.
- Taxon
- common wheatTriticum aestivumt0
- Outcome
- chromosomal fragments from bridge material and donor parent were quite often retainede0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“chromosomal fragments from bridge material and donor parent were quite often retained in intracultivaral chromosome substitution lines except the substituting chromosomes”
t0“common wheat”