Abstract
Early pregnancy detection in dairy cattle is an integral part of a successful animal husbandry practice. A simple seed germination technique (Punyakoti test) comprises observation of differential seed germination response of wheat seeds to diluted fresh urine samples as reflected by significant inhibition of germination percentage in pregnant cow urine when compared to non pregnant cow urine. Hormone metabolites excreted through urine might affect the seed germination in pregnant cow urine. In the present study an attempt was made to test the effect of hormones (in their natural forms) at different concentrations of estrogen (17-ß estradiol) and progesterone on wheat and green gram germination. Stock solutions of estrogen and progesterone were prepared in alcohol (1mg/ml) and serial dilutions made using distilled water to get the concentrations of T1=10, T2=1, T3=0.1 and T4=0.01 μg/ml respectively in treatment groups. About 15 seeds each of wheat and green gram were taken in sterile Petri dishes into which 15ml of each test preparation was poured. The treatments were compared with distilled water and alcohol controls. The study was conducted for a period of five days during which seed germination was observed after 48 hrs and shoot lengths were also measured by the end of study. The average seed germination and shoot length in treatment groups did not vary significantly (P>0.05) when compared with that of control groups. Thus from the present study, it can be concluded that estrogen and progesterone in their natural form will not affect seed germination and shoot length. [Veterinary World 2008; 1(8.000): 241-242]
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Claims 8
Claim · model-written summary
wheat seed germination did not vary significantly under estrogen (17-ß estradiol) relative to controls.
- Taxon
- wheat
Triticum aestivume0 - Treatment
- estrogen (17-ß estradiol)e2
- Outcome
- seed germinatione1
- Direction
- did not varye1
- Comparator
- control groupse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“on wheat and green gram germination.”
e1“The average seed germination and shoot length in treatment groups did not vary significantly (P>0.05) when compared with that of control groups.”
e2“estrogen (17-ß estradiol) and progesterone”
Claim · model-written summary
wheat shoot length did not vary significantly under estrogen (17-ß estradiol) relative to controls.
- Taxon
- wheat
Triticum aestivume0 - Treatment
- estrogen (17-ß estradiol)e2
- Outcome
- shoot lengthe1
- Direction
- did not varye1
- Comparator
- control groupse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“on wheat and green gram germination.”
e1“The average seed germination and shoot length in treatment groups did not vary significantly (P>0.05) when compared with that of control groups.”
e2“estrogen (17-ß estradiol) and progesterone”
Claim · model-written summary
wheat seed germination did not vary significantly under progesterone relative to controls.
- Taxon
- wheat
Triticum aestivume0 - Treatment
- progesteronee2
- Outcome
- seed germinatione1
- Direction
- did not varye1
- Comparator
- control groupse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“on wheat and green gram germination.”
e1“The average seed germination and shoot length in treatment groups did not vary significantly (P>0.05) when compared with that of control groups.”
e2“estrogen (17-ß estradiol) and progesterone”
Claim · model-written summary
wheat shoot length did not vary significantly under progesterone relative to controls.
- Taxon
- wheat
Triticum aestivume0 - Treatment
- progesteronee2
- Outcome
- shoot lengthe1
- Direction
- did not varye1
- Comparator
- control groupse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“on wheat and green gram germination.”
e1“The average seed germination and shoot length in treatment groups did not vary significantly (P>0.05) when compared with that of control groups.”
e2“estrogen (17-ß estradiol) and progesterone”
Claim · model-written summary
green gram seed germination did not vary significantly under estrogen (17-ß estradiol) relative to controls.
- Taxon
- green gram
Vigna radiata var. radiatae0 - Treatment
- estrogen (17-ß estradiol)e2
- Outcome
- seed germinatione1
- Direction
- did not varye1
- Comparator
- control groupse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“on wheat and green gram germination.”
e1“The average seed germination and shoot length in treatment groups did not vary significantly (P>0.05) when compared with that of control groups.”
e2“estrogen (17-ß estradiol) and progesterone”
Claim · model-written summary
green gram shoot length did not vary significantly under estrogen (17-ß estradiol) relative to controls.
- Taxon
- green gram
Vigna radiata var. radiatae0 - Treatment
- estrogen (17-ß estradiol)e2
- Outcome
- shoot lengthe1
- Direction
- did not varye1
- Comparator
- control groupse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“on wheat and green gram germination.”
e1“The average seed germination and shoot length in treatment groups did not vary significantly (P>0.05) when compared with that of control groups.”
e2“estrogen (17-ß estradiol) and progesterone”
Claim · model-written summary
green gram seed germination did not vary significantly under progesterone relative to controls.
- Taxon
- green gram
Vigna radiata var. radiatae0 - Treatment
- progesteronee2
- Outcome
- seed germinatione1
- Direction
- did not varye1
- Comparator
- control groupse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“on wheat and green gram germination.”
e1“The average seed germination and shoot length in treatment groups did not vary significantly (P>0.05) when compared with that of control groups.”
e2“estrogen (17-ß estradiol) and progesterone”
Claim · model-written summary
green gram shoot length did not vary significantly under progesterone relative to controls.
- Taxon
- green gram
Vigna radiata var. radiatae0 - Treatment
- progesteronee2
- Outcome
- shoot lengthe1
- Direction
- did not varye1
- Comparator
- control groupse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“on wheat and green gram germination.”
e1“The average seed germination and shoot length in treatment groups did not vary significantly (P>0.05) when compared with that of control groups.”
e2“estrogen (17-ß estradiol) and progesterone”
The same questions in other papers
estradiol · growth · Vigna radiata (mung bean)Positive and negative results
1 in the opposite direction
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.