Abstract
Dry seeds of homozygous pure breeding lines of chewing tobacco (Nicotiana tabacum L.), Sona, bandi and their F1 hybrid were treated with chemical mutagen EMS at three concentrations (0.1, 0.3 and 0.5%) for 12 h. In M1 generation, based on seed germination and seedling growth, homozygous material was found to be more sensitive than heterozygous material. Complete seed mortality was observed following treatment with 0.5% EMS. In M2 generation, range widened for all characters in different populations. Mean values increased in F2 over midparent but was significantly lowered in M2 and F2 M2 for all the characters except total cured leaf yield. There was no significant difference in mean leaf thickness which marginally decreased in F2 and increased in M2 and F2 M2. Significant increase in nicotine content was observed in F1 and M2 but in F2 M2 it decreased significantly. A significant increase in variation was evident in F2 and M2 for all characters but in F2 M2 it was so for leaf breadth and first grade leaf yield. The magnitude of the induced variation was in order of M2 < F2 < F2 M2. Variations from hybridization and mutation were generally not cumulative.
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Claims 14
Claim · model-written summary
Homozygous material was more sensitive than heterozygous material in seed germination and seedling growth.
- Taxon
- tobacco
Nicotiana tabacumt0 - Material
- homozygous materiale0
- Outcome
- seed germination and seedling growthe0
- Direction
- more sensitivee0
- Comparator
- heterozygous materiale0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“based on seed germination and seedling growth, homozygous material was found to be more sensitive than heterozygous material”
t0“tobacco”
Claim · model-written summary
EMS at 0.5% caused complete seed mortality.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- 0.5% EMSe0
- Outcome
- Complete seed mortalitye0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Complete seed mortality was observed following treatment with 0.5% EMS”
t0“tobacco”
Claim · model-written summary
Trait ranges widened in M2 populations.
- Taxon
- tobaccoNicotiana tabacumt0
- Material
- M2e0
- Outcome
- rangee0
- Direction
- widenede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In M2 generation, range widened for all characters in different populations”
t0“tobacco”
Claim · model-written summary
Trait means increased in F2 relative to midparent.
- Taxon
- tobaccoNicotiana tabacumt0
- Material
- F2e0
- Outcome
- Mean valuese0
- Direction
- increasede0
- Comparator
- midparente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mean values increased in F2 over midparent”
t0“tobacco”
Claim · model-written summary
Most trait means fell significantly in M2 and F2 M2, except cured leaf yield.
- Taxon
- tobaccoNicotiana tabacumt0
- Material
- M2 and F2 M2e0
- Outcome
- all the characters except total cured leaf yielde0
- Direction
- lowerede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“was significantly lowered in M2 and F2 M2 for all the characters except total cured leaf yield”
t0“tobacco”
Claim · model-written summary
Mean leaf thickness did not differ significantly.
- Taxon
- tobaccoNicotiana tabacumt0
- Outcome
- mean leaf thicknesse0
- Direction
- no significant differencee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“There was no significant difference in mean leaf thickness”
t0“tobacco”
Claim · model-written summary
Leaf thickness marginally decreased in F2.
- Taxon
- tobaccoNicotiana tabacumt0
- Material
- F2e0
- Outcome
- mean leaf thicknesse0
- Direction
- decreasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mean leaf thickness which marginally decreased in F2”
t0“tobacco”
Claim · model-written summary
Leaf thickness increased in M2 and F2 M2.
- Taxon
- tobaccoNicotiana tabacumt0
- Material
- M2 and F2 M2e0
- Outcome
- mean leaf thicknesse0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mean leaf thickness which marginally decreased in F2 and increased in M2 and F2 M2”
t0“tobacco”
Claim · model-written summary
Nicotine content increased significantly in F1 and M2.
- Taxon
- tobaccoNicotiana tabacumt0
- Material
- F1 and M2e0
- Outcome
- nicotine contente0
- Direction
- increasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Significant increase in nicotine content was observed in F1 and M2”
t0“tobacco”
Claim · model-written summary
Nicotine content decreased significantly in F2 M2.
- Taxon
- tobaccoNicotiana tabacumt0
- Material
- F2 M2e0
- Outcome
- nicotine contente0
- Direction
- decreasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Significant increase in nicotine content was observed in F1 and M2 but in F2 M2 it decreased significantly”
t0“tobacco”
Claim · model-written summary
Variation increased significantly for all traits in F2 and M2.
- Taxon
- tobaccoNicotiana tabacumt0
- Material
- F2 and M2e0
- Outcome
- variatione0
- Direction
- increasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A significant increase in variation was evident in F2 and M2 for all characters”
t0“tobacco”
Claim · model-written summary
In F2 M2, variation rose significantly for leaf breadth and first grade leaf yield.
- Taxon
- tobaccoNicotiana tabacumt0
- Material
- F2 M2e0
- Outcome
- leaf breadth and first grade leaf yielde0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in F2 M2 it was so for leaf breadth and first grade leaf yield”
t0“tobacco”
Claim · model-written summary
Induced variation increased in the order M2, F2, F2 M2.
- Taxon
- tobaccoNicotiana tabacumt0
- Outcome
- magnitude of the induced variatione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The magnitude of the induced variation was in order of M2 < F2 < F2 M2”
t0“tobacco”
Claim · model-written summary
Hybridization and mutation variation were generally not cumulative.
- Taxon
- tobaccoNicotiana tabacumt0
- Outcome
- Variations from hybridization and mutation were generally not cumulativee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Variations from hybridization and mutation were generally not cumulative”
t0“tobacco”