Abstract
Three plant species, spinach, juniper and broccoli, were stored in different alcohol solutions, and the DNAs were examined to determine changes in the quality and quantity of DNA over time. The smallest changes in the genomic DNA were found in the samples stored in the lower mol wt alcohols (100% methanol and 100% and 95% ethanol) and when proteinase (Pronase E) was used in the extraction procedure. After 11 months of storage in ethanol, excellent yields of high mol wt DNA were obtained, but only when the procedure utilized Pronase E. The use of proteinase was found to be essential to obtain DNA from preserved specimens of spinach and broccoli. This appears to explain the previous reports of failures to obtain DNA from alcohol-preserved specimens. Vacuum infiltration of ethanol resulted in better DNA preservation than passive infiltration of ethanol.
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Claims 4
Claim · model-written summary
Lower molecular weight alcohols and Pronase E gave the smallest genomic DNA changes.
Verbatim
e0“The smallest changes in the genomic DNA were found in the samples stored in the lower mol wt alcohols (100% methanol and 100% and 95% ethanol) and when proteinase (Pronase E) was used in the extraction procedure.”
e1“Three plant species, spinach, juniper and broccoli, were stored in different alcohol solutions”
Claim · model-written summary
After 11 months in ethanol, Pronase E extraction yielded high molecular weight DNA.
Verbatim
e0“After 11 months of storage in ethanol, excellent yields of high mol wt DNA were obtained, but only when the procedure utilized Pronase E.”
e1“Three plant species, spinach, juniper and broccoli”
Claim · model-written summary
Proteinase was essential for DNA recovery from preserved spinach and broccoli.
- Taxon
- spinach and broccoli
Spinacia oleracea
Brassica oleracea var. italicae0 - Outcome
- obtain DNA from preserved specimense0
- Value
- essentiale0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The use of proteinase was found to be essential to obtain DNA from preserved specimens of spinach and broccoli.”
Claim · model-written summary
Vacuum infiltration improved DNA preservation relative to passive infiltration.
Verbatim
e0“Vacuum infiltration of ethanol resulted in better DNA preservation than passive infiltration of ethanol.”
e1“Three plant species, spinach, juniper and broccoli”