Abstract
Elimination Sugarcane Mozaic Virus (SCMV) in sugarcane plantlets and propagation via somatic embryogenesis was assessed. The aim of this study was to obtain a virus-free sugarcane and determine methods for propagation via somatic embryogenesis. Explants derived from sugarcane SUT-02 plantlets. The explants were cultured on ribavirin and acyclovir medium at 0, 20 and 40 mgl-1 concentrations. Virus-free plantlets detected by DAS-ELISA. Rapid propagation virus-free plantlets through somatic embryogenesis consists of 3 stages. Stage 1: callus induction on the media (a) MSo, (b) MSo + 3 mgl-1 2,4-D, (c) MSo + 4 mgl-1 2,4-D, (d) MSo + 3 mgl-1 2,4-D + 1.5 mgl-1 BAP, (e) MSo + 4 mgl-1 2,4-D + 1.5 mgl-1 BAP, stage 2: proliferation on media MSo + 1.5 mgl-1 2,4-D and stage 3: regeneration on MSo media. The results showed that the media ribavirin and acyclovir at 20 mgl-1 and 40 mgl-1 concentration produces 100% virus-free sugarcane plantlets. Best embryogenic callus induction media is MSo + 3 mgl-1 2,4-D and virus free plantlets have produced as much as ± 35 plantlets within 9 weeks.
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Claims 6
Claim · model-written summary
Ribavirin at 20 mgl-1 produced 100% virus-free sugarcane plantlets.
- Taxon
- sugarcanee0
- Treatment
- ribavirine0
- Interactor
- Sugarcane Mozaic Virus (SCMV)
Sugarcane mosaic viruse1pathogen - Outcome
- virus-free sugarcane plantletse0
- Value
- 100%e0
- Qualifier
- 20 mgl-1e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the media ribavirin and acyclovir at 20 mgl-1 and 40 mgl-1 concentration produces 100% virus-free sugarcane plantlets”
e1“Sugarcane Mozaic Virus (SCMV)”
Claim · model-written summary
Ribavirin at 40 mgl-1 produced 100% virus-free sugarcane plantlets.
- Taxon
- sugarcanee0
- Treatment
- ribavirine0
- Interactor
- Sugarcane Mozaic Virus (SCMV)Sugarcane mosaic viruse1pathogen
- Outcome
- virus-free sugarcane plantletse0
- Value
- 100%e0
- Qualifier
- 40 mgl-1e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the media ribavirin and acyclovir at 20 mgl-1 and 40 mgl-1 concentration produces 100% virus-free sugarcane plantlets”
e1“Sugarcane Mozaic Virus (SCMV)”
Claim · model-written summary
Acyclovir at 20 mgl-1 produced 100% virus-free sugarcane plantlets.
- Taxon
- sugarcanee0
- Treatment
- acyclovire0
- Interactor
- Sugarcane Mozaic Virus (SCMV)Sugarcane mosaic viruse1pathogen
- Outcome
- virus-free sugarcane plantletse0
- Value
- 100%e0
- Qualifier
- 20 mgl-1e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the media ribavirin and acyclovir at 20 mgl-1 and 40 mgl-1 concentration produces 100% virus-free sugarcane plantlets”
e1“Sugarcane Mozaic Virus (SCMV)”
Claim · model-written summary
Acyclovir at 40 mgl-1 produced 100% virus-free sugarcane plantlets.
- Taxon
- sugarcanee0
- Treatment
- acyclovire0
- Interactor
- Sugarcane Mozaic Virus (SCMV)Sugarcane mosaic viruse1pathogen
- Outcome
- virus-free sugarcane plantletse0
- Value
- 100%e0
- Qualifier
- 40 mgl-1e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the media ribavirin and acyclovir at 20 mgl-1 and 40 mgl-1 concentration produces 100% virus-free sugarcane plantlets”
e1“Sugarcane Mozaic Virus (SCMV)”
Claim · model-written summary
MSo plus 3 mgl-1 2,4-D was the best medium for embryogenic sugarcane callus induction.
Not reportedComparator
Verbatim
e0“Best embryogenic callus induction media is MSo + 3 mgl-1 2,4-D”
e1“virus-free sugarcane plantlets”
Claim · model-written summary
Somatic embryogenesis produced approximately 35 virus-free sugarcane plantlets within nine weeks.
Verbatim
e0“virus free plantlets have produced as much as ± 35 plantlets within 9 weeks.”
e1“determine methods for propagation via somatic embryogenesis”
e2“virus-free sugarcane plantlets”
The same questions in other papers
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- positive
- positive
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See all 38 papers →acyclovir · Sugarcane mosaic virus · Saccharum officinarum (sugarcane)Results agree
1 paper in the same direction as this paper
- positive
ribavirin · Sugarcane mosaic virus · Saccharum officinarum (sugarcane)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.