Abstract
In order to evaluate the response of bread wheat genotypes to callus induction and in vitro drought stress, an investigation was carried out as a factorial experiment with a completely randomized design and five replications. The results of analysis of variance indicated significant differences between the entries and stress levels for callus relative growth (CRG), callus relative growth rate (CRGR), callus growth rate (CGR), percentage of callus chlorosis (PCCH) and percentage of callus water content (PCWC) indicating the presence of genetic variability, different responses of genotypes to different drought intensities and invitro selection of droughttolerant genotypes. Mean comparisons between genotypes revealed that maximum CRG, CGR, CRGR, PCWG, PCCH and INTOL were attributed to genotypes 5, 16, 17, 2, 3 and 20 (drought tolerant), respectively. Graphic observation exhibited that indices of drought tolerance decreased with increase of PEG concentrations. Cluster analysis of genotypes (Ward’s method) based on CRG, CGR, CRGR, PCWG, PCCH and INTOL and subsequent discriminant analysis for confirming the number of clusters, grouped the genotypes into four different clusters. The first group included genotypes 1, 3, 6, 7, 12, 13 and 18, the second group included genotypes 2, 4, 8, 9, 10 and 11 and the third group consisted of genotypes 14, 16, 17, 19 and 20, while the genotype 15 formed the fourth group. Superior genotypes 2, 5 and 16 showed drought tolerance at the callus culture level together with their high potential for callus induction leaded us to the conclusion that a hybridization breeding program using these superior plant materials supplemented with in vitro selection for drought tolerance might be beneficial for improvement drought tolerance in bread wheat.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 11
Claim · model-written summary
Genotypes and drought stress levels differed significantly for callus relative growth, growth rate, chlorosis and water content.
- Taxon
- bread wheat
Triticum aestivumt0 - Treatment
- stress levelse0
- Outcome
- callus relative growth (CRG), callus relative growth rate (CRGR), callus growth rate (CGR)e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“significant differences between the entries and stress levels for callus relative growth (CRG), callus relative growth rate (CRGR), callus growth rate (CGR), percentage of callus chlorosis (PCCH) and percentage of callus water content (PCWC)”
t0“bread wheat”
Claim · model-written summary
Genotype 5 had maximum callus relative growth.
- Taxon
- bread wheatTriticum aestivumt0
- Material
- genotypes 5e0
- Outcome
- CRGe0
- Direction
- maximume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“maximum CRG, CGR, CRGR, PCWG, PCCH and INTOL were attributed to genotypes 5, 16, 17, 2, 3 and 20 (drought tolerant), respectively”
t0“bread wheat”
Claim · model-written summary
Genotype 16 had maximum callus growth rate.
- Taxon
- bread wheatTriticum aestivumt0
- Material
- 16e0
- Outcome
- CGRe0
- Direction
- maximume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“maximum CRG, CGR, CRGR, PCWG, PCCH and INTOL were attributed to genotypes 5, 16, 17, 2, 3 and 20 (drought tolerant), respectively”
t0“bread wheat”
Claim · model-written summary
Genotype 17 had maximum callus relative growth rate.
- Taxon
- bread wheatTriticum aestivumt0
- Material
- 17e0
- Outcome
- CRGRe0
- Direction
- maximume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“maximum CRG, CGR, CRGR, PCWG, PCCH and INTOL were attributed to genotypes 5, 16, 17, 2, 3 and 20 (drought tolerant), respectively”
t0“bread wheat”
Claim · model-written summary
Genotype 2 had maximum PCWG.
- Taxon
- bread wheatTriticum aestivumt0
- Material
- 2e0
- Outcome
- PCWGe0
- Direction
- maximume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“maximum CRG, CGR, CRGR, PCWG, PCCH and INTOL were attributed to genotypes 5, 16, 17, 2, 3 and 20 (drought tolerant), respectively”
t0“bread wheat”
Claim · model-written summary
Genotype 3 had maximum callus chlorosis percentage.
- Taxon
- bread wheatTriticum aestivumt0
- Material
- 3e0
- Outcome
- PCCHe0
- Direction
- maximume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“maximum CRG, CGR, CRGR, PCWG, PCCH and INTOL were attributed to genotypes 5, 16, 17, 2, 3 and 20 (drought tolerant), respectively”
t0“bread wheat”
Claim · model-written summary
Genotype 20 had maximum INTOL.
- Taxon
- bread wheatTriticum aestivumt0
- Material
- 20e0
- Outcome
- INTOLe0
- Direction
- maximume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“maximum CRG, CGR, CRGR, PCWG, PCCH and INTOL were attributed to genotypes 5, 16, 17, 2, 3 and 20 (drought tolerant), respectively”
t0“bread wheat”
Claim · model-written summary
Drought tolerance indices decreased as PEG concentrations increased.
- Taxon
- bread wheatTriticum aestivumt0
- Treatment
- PEG concentrationse0
- Outcome
- indices of drought tolerancee0
- Direction
- decreasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“indices of drought tolerance decreased with increase of PEG concentrations”
t0“bread wheat”
Claim · model-written summary
Cluster and discriminant analyses grouped the genotypes into four clusters.
- Taxon
- bread wheatTriticum aestivumt0
- Outcome
- four different clusterse0
- Value
- foure0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“grouped the genotypes into four different clusters”
t0“bread wheat”
Claim · model-written summary
Genotypes 2, 5 and 16 were superior for drought tolerance at callus level and callus induction potential.
- Taxon
- bread wheatTriticum aestivumt0
- Material
- genotypes 2, 5 and 16e0
- Outcome
- drought tolerance at the callus culture levele0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Superior genotypes 2, 5 and 16 showed drought tolerance at the callus culture level together with their high potential for callus induction”
t0“bread wheat”
Claim · model-written summary
The authors suggest hybridization using the superior genotypes with in vitro selection to improve drought tolerance.
- Taxon
- bread wheatTriticum aestivume0
- Outcome
- a hybridization breeding program using these superior plant materials supplemented with in vitro selection for drought tolerancee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a hybridization breeding program using these superior plant materials supplemented with in vitro selection for drought tolerance might be beneficial for improvement drought tolerance in bread wheat”