Abstract
In order to optimize the method of 2n pollen induction and to test the effects of colchicine on 2n pollen formation in nectarine,2n pollen was induced by two colchicine treatments,cotton wool soaking and direct injection into floral buds of Prunus persica(L.)Batsch.var.nectarina(Ait.)Maxim.'Lichun'.Orthogonal experimental design was used in this study.In treatment of direct colchicine injection,2n pollen ratio mounted to 85.0%,but fewer flowers developed.In treatment of cotton wool soaking,2n pollen ratio was 44.1%,and the ratio of full developed flower was.The duration of treatment and humectant concentration were the key factors in cotton wool soaking.The maximum 2n pollen ratio was obtained when cotton wool soaking treatment lasted 32 hours and the 1% glycerol was used as humectant.Concerning to the ratio of flower harvested and 2n pollen percentage,the optimum method for 2n pollen induction in nectarine is 0.1%-0.2% colchicine treatment by cotton wool soaking for 24-32 h with 1 mL of laundry powder as saturation and 1% glycerol as humectant respectively.
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Claims 5
Claim · model-written summary
Direct colchicine injection into floral buds raised the 2n pollen ratio to 85.0% in nectarine.
- Taxon
- nectarine
Prunus persica var. nucipersicae0 - Treatment
- direct colchicine injectione1
- Outcome
- 2n pollen ratioe1
- Direction
- increaseinferrede1
- Value
- 85.0%e1
- Comparator
- cotton wool soakinge3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“to test the effects of colchicine on 2n pollen formation in nectarine”
e1“In treatment of direct colchicine injection,2n pollen ratio mounted to 85.0%”
e3“In treatment of cotton wool soaking,2n pollen ratio was 44.1%”
Claim · model-written summary
Direct colchicine injection resulted in fewer developed flowers in nectarine.
- Taxon
- nectarinePrunus persica var. nucipersicae0
- Treatment
- direct colchicine injectione1
- Outcome
- flowers developede2
- Direction
- fewere2
- Comparator
- cotton wool soakinge3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“to test the effects of colchicine on 2n pollen formation in nectarine”
e1“In treatment of direct colchicine injection,2n pollen ratio mounted to 85.0%”
e2“but fewer flowers developed”
e3“In treatment of cotton wool soaking,2n pollen ratio was 44.1%”
Claim · model-written summary
Cotton wool soaking raised the 2n pollen ratio to 44.1% in nectarine.
- Taxon
- nectarinePrunus persica var. nucipersicae0
- Treatment
- cotton wool soakinge3
- Outcome
- 2n pollen ratioe3
- Direction
- increaseinferrede3
- Value
- 44.1%e3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“to test the effects of colchicine on 2n pollen formation in nectarine”
e3“In treatment of cotton wool soaking,2n pollen ratio was 44.1%”
Claim · model-written summary
The maximum 2n pollen ratio in nectarine was obtained with cotton wool soaking lasting 32 hours and 1% glycerol as humectant.
- Taxon
- nectarinePrunus persica var. nucipersicae0
- Treatment
- cotton wool soaking treatment lasted 32 hourse4
- Outcome
- 2n pollen ratioe4
- Direction
- maximume4
- Qualifier
- 1% glycerol was used as humectante4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“to test the effects of colchicine on 2n pollen formation in nectarine”
e4“The maximum 2n pollen ratio was obtained when cotton wool soaking treatment lasted 32 hours and the 1% glycerol was used as humectant”
Claim · model-written summary
The optimum method for 2n pollen induction in nectarine is 0.1%-0.2% colchicine by cotton wool soaking for 24-32 h.
- Taxon
- nectarinePrunus persica var. nucipersicae5
- Treatment
- 0.1%-0.2% colchicine treatment by cotton wool soaking for 24-32 he5
- Outcome
- 2n pollen inductione5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“the optimum method for 2n pollen induction in nectarine is 0.1%-0.2% colchicine treatment by cotton wool soaking for 24-32 h”
The same questions in other papers
application · phenology · Prunus persica (peach)Positive and negative results
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