Abstract
The evaluation of transgenic plants which is usually carried out under controlled conditions in culture rooms and greenhouses can yield valuable information about the influence of introduced genes on a transgenic plant phenotype. However, an overall assessment of plant performance can only be made by testing transgenic plants in the field environment. Thus, the effects of pyramided rice cystatin genes OCI and OCII on morphological parameters of transgenic potato cv. Desiree, Dragacevka and Jelica lines were compared under in vitro, greenhouse, and field conditions. All analyzed OC co-expressing transgenic lines exhibited normal phenotype, both in vitro and in greenhouse conditions. In the field environment, eight of nine OCI/OCII lines were similar to the wild-type control plants in their general phenotypic appearance. Yield parameters, such as tuber number and tuber weight for these phenotypically normal OCI/OCII lines, were also comparable to the controls. Only transgenic cv. Jelica line 4 plants exhibited slightly reduced growth, atypical leaf morphology and, contrary to the plants of other transgenic lines and untransformed controls, failed to flower. However, despite the phenotypic and developmental changes under field conditions, the OCI/OCII Jelica line 4 did not exhibit a significant decrease in tuber yield. Stacking of OCI and OCII genes preserves important attributes of the parental lines, confirming that this approach could be suitable for improving agronomical traits in potato.
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Claims 6
Claim · model-written summary
All OC co-expressing lines had normal phenotype in vitro and in the greenhouse.
- Taxon
- potato
Solanum tuberosumt0 - Material
- OC co-expressing transgenic linese0
- Outcome
- normal phenotypee0
- Setting
- in vitro and in greenhouse conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“All analyzed OC co-expressing transgenic lines exhibited normal phenotype, both in vitro and in greenhouse conditions.”
t0“Phenotypic performance of transgenic potato (Solanum tuberosum L.) plants with pyramided rice cystatin genes (OCI and OCII)”
Claim · model-written summary
Eight of nine OCI/OCII lines resembled wild-type controls in field appearance.
- Taxon
- potatoSolanum tuberosumt0
- Material
- OCI/OCII linese0
- Outcome
- general phenotypic appearancee0
- Value
- eight of ninee0
- Comparator
- wild-type control plantse0
- Setting
- field environmente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In the field environment, eight of nine OCI/OCII lines were similar to the wild-type control plants in their general phenotypic appearance.”
t0“Phenotypic performance of transgenic potato (Solanum tuberosum L.) plants with pyramided rice cystatin genes (OCI and OCII)”
Claim · model-written summary
Phenotypically normal lines had tuber number and weight comparable to controls.
- Taxon
- potatoSolanum tuberosumt0
- Material
- OCI/OCII linese0
- Outcome
- tuber number and tuber weighte0
- Comparator
- controlse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Yield parameters, such as tuber number and tuber weight for these phenotypically normal OCI/OCII lines, were also comparable to the controls.”
t0“Phenotypic performance of transgenic potato (Solanum tuberosum L.) plants with pyramided rice cystatin genes (OCI and OCII)”
Claim · model-written summary
Jelica line 4 showed slightly reduced growth and atypical leaf morphology.
- Taxon
- potatoSolanum tuberosumt0
- Material
- cv. Jelica line 4e0
- Outcome
- growth, atypical leaf morphologye0
- Direction
- slightly reducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Only transgenic cv. Jelica line 4 plants exhibited slightly reduced growth, atypical leaf morphology”
t0“Phenotypic performance of transgenic potato (Solanum tuberosum L.) plants with pyramided rice cystatin genes (OCI and OCII)”
Claim · model-written summary
Jelica line 4 failed to flower compared with other lines and controls.
- Taxon
- potatoSolanum tuberosumt0
- Material
- cv. Jelica line 4e1
- Outcome
- flowere0
- Direction
- failed toe0
- Comparator
- other transgenic lines and untransformed controlse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“contrary to the plants of other transgenic lines and untransformed controls, failed to flower.”
t0“Phenotypic performance of transgenic potato (Solanum tuberosum L.) plants with pyramided rice cystatin genes (OCI and OCII)”
e1“cv. Jelica line 4”
Claim · model-written summary
Jelica line 4 had no significant decrease in tuber yield under field conditions.
- Taxon
- potatoSolanum tuberosumt0
- Material
- OCI/OCII Jelica line 4e0
- Outcome
- tuber yielde0
- Direction
- did not exhibit a significant decreasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the OCI/OCII Jelica line 4 did not exhibit a significant decrease in tuber yield.”
t0“Phenotypic performance of transgenic potato (Solanum tuberosum L.) plants with pyramided rice cystatin genes (OCI and OCII)”