Abstract
Although the use of plant growth-promoting bacteria in agriculture is a reality, the molecular basis of plant-bacterial interaction is still poorly understood. We used a proteomic approach to study the mechanisms of interaction of Herbaspirillum seropedicae SmR1 with rice. Root proteins of rice seedlings inoculated or noninoculated with H. seropedicae were separated by 2-D electrophoresis. Differentially expressed proteins were identified by MALDI-TOF/TOF and MASCOT program. Among the identified proteins of H. seropedicae, the dinitrogenase reductase NifH and glutamine synthetase GlnA, which participate in nitrogen fixation and ammonium assimilation, respectively, were the most abundant. The rice proteins up-regulated included the S-adenosylmethionine synthetase, methylthioribose kinase, and acireductone dioxygenase 1, all of which are involved in the methionine recycling. S-Adenosylmethionine synthetase catalyzes the synthesis of S-adenosylmethionine, an intermediate used in transmethylation reactions and in ethylene, polyamine, and phytosiderophore biosynthesis. RT-qPCR analysis also confirmed that the methionine recycling and phytosiderophore biosynthesis genes were up-regulated, while ACC oxidase mRNA level was down-regulated in rice roots colonized by bacteria. In agreement with these results, ethylene production was reduced approximately three-fold in rice roots colonized by H. seropedicae. The results suggest that H. seropedicae stimulates methionine recycling and phytosiderophore synthesis and diminishes ethylene synthesis in rice roots.
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Claims 5
Claim · model-written summary
NifH and GlnA were the most abundant identified H. seropedicae proteins in the colonized rice roots.
- Taxon
- Rice
Oryza sativat0 - Interactor
- H. seropedicaeHerbaspirillum seropedicaee0symbiont
- Gene
- dinitrogenase reductase NifH and glutamine synthetase GlnAe0
- Outcome
- most abundante0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Among the identified proteins of H. seropedicae, the dinitrogenase reductase NifH and glutamine synthetase GlnA, which participate in nitrogen fixation and ammonium assimilation, respectively, were the most abundant.”
t0“Rice Roots”
Claim · model-written summary
S-adenosylmethionine synthetase, methylthioribose kinase and acireductone dioxygenase 1 were up-regulated in rice roots.
- Taxon
- RiceOryza sativat0
- Treatment
- Colonized byHerbaspirillum seropedicaeStrain SmR1t0
- Interactor
- Herbaspirillum seropedicaeHerbaspirillum seropedicaet0symbiont
- Gene
- S-adenosylmethionine synthetase, methylthioribose kinase, and acireductone dioxygenase 1e0
- Outcome
- rice proteinse0
- Direction
- up-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The rice proteins up-regulated included the S-adenosylmethionine synthetase, methylthioribose kinase, and acireductone dioxygenase 1, all of which are involved in the methionine recycling.”
t0“Rice Roots Colonized byHerbaspirillum seropedicaeStrain SmR1”
Claim · model-written summary
Methionine recycling and phytosiderophore biosynthesis genes were up-regulated in colonized rice roots.
- Taxon
- riceOryza sativae0
- Treatment
- colonized by bacteriae0
- Outcome
- methionine recycling and phytosiderophore biosynthesis genese0
- Direction
- up-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RT-qPCR analysis also confirmed that the methionine recycling and phytosiderophore biosynthesis genes were up-regulated, while ACC oxidase mRNA level was down-regulated in rice roots colonized by bacteria.”
Claim · model-written summary
ACC oxidase mRNA level was down-regulated in colonized rice roots.
- Taxon
- riceOryza sativae0
- Treatment
- colonized by bacteriae0
- Gene
- ACC oxidasee0
- Outcome
- ACC oxidase mRNA levele0
- Direction
- down-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ACC oxidase mRNA level was down-regulated in rice roots colonized by bacteria.”
Claim · model-written summary
Ethylene production in colonized rice roots was reduced approximately three-fold.
- Taxon
- riceOryza sativae0
- Treatment
- colonized by H. seropedicaee0
- Interactor
- H. seropedicaeHerbaspirillum seropedicaee0symbiont
- Outcome
- ethylene productione0
- Direction
- reducede0
- Value
- approximately three-folde0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ethylene production was reduced approximately three-fold in rice roots colonized by H. seropedicae.”
The same questions in other papers
inoculation · Bacteria · Oryza sativa (rice)Positive and negative results
11 papers in the same direction as this paper · 3 in the opposite direction
- negative
- negative
- no effect
11 papers more in this group
See all 15 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.