Abstract
SummarySweet potato (Ipomoea batatas L. Lam.) is a staple root crop, consumed as a carbohydrate-rich food source in many tropical countries. The production and productivity of sweet potato is limited by several factors, mainly virus infections. This study was conducted to produce virus-free sweet potato planting material using meristem culture. Meristems without leaf primordia were excised from four virus-positive field-grown local sweet potato cultivars ‘Bellela’, ‘Temesgen’, ‘LO-323’, and ‘Zapallo’. Meristems were cultured in Murashige and Skoog (MS) medium supplemented with different combinations and concentrations of 6-benzylaminopurine (BAP), α-naphthaleneacetic acid (NAA), and gibberellic acid (GA3) for shoot initiation. ‘Bellela’ and ‘Temesgen’ exhibited 100% shoot initiation on 5 mg l–1 BAP, 0.01 mg l–1 NAA, and 1 mg l–1 GA3, although the shoots were bushy. ‘LO-323’ showed 90% shoot initiation with the best shoot quality on 2 mg l–1 BAP, 0.01 mg l–1 NAA, and 1 mg l–1 GA3. Shoot tips and nodes from in vitro-grown plantlets were cultured on BAP (0, 0.5, 1.0, or 2.0 mg l–1) or on BAP (0, 0.5, 1.0, or 2.0 mg l–1) + kinetin (Kin; 0, 0.1, 0.5 or 1.0 mg l–1) for multiple shoot formation. The best shoot proliferation was obtained on MS + 0.5 mg l–1 BAP + 0.5 mg l–1 Kin. In vitro-grown shoots were cultured on MS medium containing 0.0, 0.01, 0.1, 0.5, or 1.0 mgl–1 indole-3-butyric acid (IBA). The best root formation was obtained on IBA-free medium. The plantlets thus produced were tested for the presence of ten sweet potato viruses [Sweet potato chlorotic stunt virus (SPCSV), Sweet potato feathery mottle virus (SPFMV), Sweet potato mild mottle virus (SPMMV), Sweet potato chlorotic fleck virus (SPCFV), Sweet potato caulimo-like virus (SPCaLV), Sweet potato mild speckling virus (SPMSV), C-6 (a flexuous rod virus), Sweet potato latent virus (SwPLV), Sweet potato virus G (SPVG), and Cucumber mosaic virus (CMV)] using nitrocellulose membrane enzyme-linked immunosorbent assay (NCM-ELISA). Ninety-nine percent of plantlets were free from all viruses.
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Claims 6
Claim · model-written summary
Sweet potato cultivar Bellela showed 100% shoot initiation from meristems on 5 mg/l BAP, 0.01 mg/l NAA and 1 mg/l GA3, although the shoots were bushy.
- Taxon
- sweet potato
_Flower.jpg?width=60)
Ipomoea batatast0 - Material
- Bellelae0
- Treatment
- 5 mg l–1 BAP, 0.01 mg l–1 NAA, and 1 mg l–1 GA3e0
- Outcome
- shoot initiatione0
- Value
- 100%e0
- Qualifier
- although the shoots were bushye0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Flower.jpg?width=500)
Verbatim
t0“sweet potato”
e0“‘Bellela’ and ‘Temesgen’ exhibited 100% shoot initiation on 5 mg l–1 BAP, 0.01 mg l–1 NAA, and 1 mg l–1 GA3, although the shoots were bushy”
Claim · model-written summary
Sweet potato cultivar Temesgen showed 100% shoot initiation from meristems on 5 mg/l BAP, 0.01 mg/l NAA and 1 mg/l GA3, although the shoots were bushy.
- Taxon
- sweet potatoIpomoea batatast0
- Material
- Temesgene0
- Treatment
- 5 mg l–1 BAP, 0.01 mg l–1 NAA, and 1 mg l–1 GA3e0
- Outcome
- shoot initiatione0
- Value
- 100%e0
- Qualifier
- although the shoots were bushye0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“sweet potato”
e0“‘Bellela’ and ‘Temesgen’ exhibited 100% shoot initiation on 5 mg l–1 BAP, 0.01 mg l–1 NAA, and 1 mg l–1 GA3, although the shoots were bushy”
Claim · model-written summary
Cultivar LO-323 showed 90% shoot initiation with the best shoot quality on 2 mg/l BAP, 0.01 mg/l NAA and 1 mg/l GA3.
- Taxon
- sweet potatoIpomoea batatast0
- Material
- LO-323e0
- Treatment
- 2 mg l–1 BAP, 0.01 mg l–1 NAA, and 1 mg l–1 GA3e0
- Outcome
- shoot initiatione0
- Value
- 90%e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“sweet potato”
e0“‘LO-323’ showed 90% shoot initiation with the best shoot quality on 2 mg l–1 BAP, 0.01 mg l–1 NAA, and 1 mg l–1 GA3”
Claim · model-written summary
The best shoot proliferation was obtained on MS + 0.5 mg/l BAP + 0.5 mg/l kinetin.
- Taxon
- sweet potatoIpomoea batatast0
- Treatment
- MS + 0.5 mg l–1 BAP + 0.5 mg l–1 Kine0
- Outcome
- shoot proliferatione0
- Direction
- beste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“sweet potato”
e0“The best shoot proliferation was obtained on MS + 0.5 mg l–1 BAP + 0.5 mg l–1 Kin”
Claim · model-written summary
The best root formation was obtained on IBA-free medium.
- Taxon
- sweet potatoIpomoea batatast0
- Treatment
- IBA-free mediume0
- Outcome
- root formatione0
- Direction
- beste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“sweet potato”
e0“The best root formation was obtained on IBA-free medium”
Claim · model-written summary
Ninety-nine percent of the plantlets produced by meristem culture were free from all ten tested viruses.
- Taxon
- sweet potatoIpomoea batatast0
- Outcome
- free from all virusese0
- Value
- Ninety-nine percente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“sweet potato”
e0“Ninety-nine percent of plantlets were free from all viruses”
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