Abstract
Sweet potato shoot apical meristems with 1-2 leaf primordia were aseptically isolated and cultured on liquid or solid Murashige and Skoog (MS) medium supplemented with different plant growth regulators. Primary shoot induction was most effectively promoted by liquid MS medium supplemented with 2.0 mg/l Kn plus 0.5mg/l GA 3. In this combination about 75% of excised meristems responded with an average vigor of 0.79. The shoots were regenerated without intervening any callus formation. The use of TDZ or BAP produced callus instead of shoot development and therefore not recommended for meristem culture. Following primary establishment, the primary shoots were further cultured on semisolid MS medium supplemented with 2.5mg/l Kn+0.5mg/l GA 3 for proper shoot growth. The developed shoots were further micropropagated by node cutting. For optimal micropropagation by node cutting, MS medium supplemented with 3.0mg/l Kn+0.5mg/l GA 3 was most effective. More than 75% of these micropropagated plantlets were successfully established and showed new leaf development under soil condition. This optimized meristem culture technique would be useful for developing uniform and virus-free clones of sweet potato in which viral diseases are predominant.
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Claims 10
Claim · model-written summary
Liquid MS medium with 2.0 mg/l Kn and 0.5mg/l GA 3 most effectively promoted primary shoot induction.
- Taxon
- Sweet Potato
_Flower.jpg?width=60)
Ipomoea batatast0 - Treatment
- liquid MS medium supplemented with 2.0 mg/l Kn plus 0.5mg/l GA 3e0
- Outcome
- Primary shoot inductione0
- Direction
- most effectively promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Flower.jpg?width=500)
Verbatim
t0“Sweet Potato”
e0“Primary shoot induction was most effectively promoted by liquid MS medium supplemented with 2.0 mg/l Kn plus 0.5mg/l GA 3.”
Claim · model-written summary
About 75% of excised meristems responded under the primary shoot induction treatment.
- Taxon
- Sweet PotatoIpomoea batatast0
- Outcome
- excised meristems respondede0
- Value
- 75%e0
- Qualifier
- In this combinatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Sweet Potato”
e0“In this combination about 75% of excised meristems responded with an average vigor of 0.79.”
Claim · model-written summary
Meristem vigor averaged 0.79 under this combination.
- Taxon
- Sweet PotatoIpomoea batatast0
- Outcome
- average vigore0
- Value
- 0.79e0
- Qualifier
- In this combinatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Sweet Potato”
e0“In this combination about 75% of excised meristems responded with an average vigor of 0.79.”
Claim · model-written summary
Shoots regenerated without callus formation.
- Taxon
- Sweet PotatoIpomoea batatast0
- Outcome
- shoots were regenerated without intervening any callus formatione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Sweet Potato”
e0“The shoots were regenerated without intervening any callus formation.”
Claim · model-written summary
TDZ produced callus instead of shoot development.
- Taxon
- Sweet PotatoIpomoea batatast0
- Treatment
- TDZe0
- Outcome
- callus instead of shoot developmente0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Sweet Potato”
e0“The use of TDZ or BAP produced callus instead of shoot development”
Claim · model-written summary
BAP produced callus instead of shoot development.
- Taxon
- Sweet PotatoIpomoea batatast0
- Treatment
- BAPe0
- Outcome
- callus instead of shoot developmente0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Sweet Potato”
e0“The use of TDZ or BAP produced callus instead of shoot development”
Claim · model-written summary
MS medium with 3.0mg/l Kn and 0.5mg/l GA 3 was most effective for node cutting micropropagation.
- Taxon
- Sweet PotatoIpomoea batatast0
- Treatment
- MS medium supplemented with 3.0mg/l Kn+0.5mg/l GA 3e0
- Outcome
- micropropagation by node cuttinge0
- Direction
- most effectivee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Sweet Potato”
e0“For optimal micropropagation by node cutting, MS medium supplemented with 3.0mg/l Kn+0.5mg/l GA 3 was most effective.”
Claim · model-written summary
More than 75% of micropropagated plantlets established in soil and developed new leaves.
- Taxon
- Sweet PotatoIpomoea batatast0
- Outcome
- successfully established and showed new leaf developmente0
- Value
- More than 75%e0
- Setting
- soil conditione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Sweet Potato”
e0“More than 75% of these micropropagated plantlets were successfully established and showed new leaf development under soil condition.”
Claim · model-written summary
The authors do not recommend TDZ for sweet potato meristem culture.
- Taxon
- Sweet PotatoIpomoea batatast0
- Treatment
- TDZe0
- Outcome
- not recommended for meristem culturee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Sweet Potato”
e0“The use of TDZ or BAP produced callus instead of shoot development and therefore not recommended for meristem culture.”
Claim · model-written summary
The authors do not recommend BAP for sweet potato meristem culture.
- Taxon
- Sweet PotatoIpomoea batatast0
- Treatment
- BAPe0
- Outcome
- not recommended for meristem culturee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Sweet Potato”
e0“The use of TDZ or BAP produced callus instead of shoot development and therefore not recommended for meristem culture.”
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