Abstract
Salt stress seriously affected the production of soybean.Thus screening salt-tolerant soybeans is very important for developing salt resistant cultivars.The objective of this study was to establish a simple method for screening soybean varieties in response to salt stress.The seedlings of four salt-tolerant varieties and four salt-sensitive varieties were grown in vermiculite pouring with 0,100,200,and 250 mmol L-1 NaCl solutions,adding each two days for three times.The SPAD was measured every day.The root,stem and leaf were sampled after eight days of treatment and Na+ content were measured by the atomic absorption spectrophotometer.The results indicated that it is easy to distinguish the level of salt tolerance based on foliar symptoms in both treatment of 200 mmol L-1 and 250 mmol L-1 NaCl.Under 250 mmol L-1 NaCl stress,the Na+ content in leaf for salt-tolerant varieties was lower than that for salt-sensitive varieties.There was a significant correlation between Na+ content and SPAD in salt-sensitive varieties.This experiment established a convenient method of soybean salinity tolerance identification at seedling stage,using the seedlings grown in vermiculite with 200 mmol L-1 and 250 mmol L-1 NaCl treatment for eight days.It will facili-tate the screening of germplasm,mechanism study and breeding selection on a large scale.
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Claims 4
Claim · model-written summary
Salt tolerance level of soybean varieties was easy to distinguish from foliar symptoms under both 200 and 250 mmol L-1 NaCl treatments.
- Taxon
- Soybean
Glycine maxt0 - Treatment
- 200 mmol L-1 and 250 mmol L-1 NaCle0
- Outcome
- distinguish the level of salt tolerance based on foliar symptomse0
- Value
- 200 mmol L-1 and 250 mmol L-1 NaCle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Soybean”
e0“it is easy to distinguish the level of salt tolerance based on foliar symptoms in both treatment of 200 mmol L-1 and 250 mmol L-1 NaCl”
Claim · model-written summary
Under 250 mmol L-1 NaCl, leaf Na+ content was lower in salt-tolerant than in salt-sensitive soybean varieties.
- Taxon
- SoybeanGlycine maxt0
- Material
- salt-tolerant varietiese1
- Organ / stage
- leafe1
- Treatment
- 250 mmol L-1 NaCl stresse1
- Outcome
- Na+ content in leafe1
- Direction
- lowere1
- Comparator
- salt-sensitive varietiese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Soybean”
e1“Under 250 mmol L-1 NaCl stress,the Na+ content in leaf for salt-tolerant varieties was lower than that for salt-sensitive varieties”
Claim · model-written summary
Na+ content and SPAD were significantly correlated in salt-sensitive soybean varieties.
- Taxon
- SoybeanGlycine maxt0
- Material
- salt-sensitive varietiese2
- Outcome
- correlation between Na+ content and SPADe2
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Soybean”
e2“There was a significant correlation between Na+ content and SPAD in salt-sensitive varieties”
Claim · model-written summary
A convenient method of soybean salinity tolerance identification at seedling stage was established using seedlings grown in vermiculite with 200 and 250 mmol L-1 NaCl for eight days.
- Taxon
- SoybeanGlycine maxt0
- Outcome
- convenient method of soybean salinity tolerance identificatione3
- Value
- eight dayse3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Soybean”
e3“established a convenient method of soybean salinity tolerance identification at seedling stage,using the seedlings grown in vermiculite with 200 mmol L-1 and 250 mmol L-1 NaCl treatment for eight days”