Paper · 1938 · Canadian Journal of Research/Canadian journal of research

HYBRIDIZATION OF TRITICUM AND AGROPYRON: V. DOUBLING THE CHROMOSOME NUMBER IN T. VULGARE AND F1 OF T. VULGARE × A. GLAUCUM BY TEMPERATURE TREATMENTS

F. H. Peto

6
claims
11
verbatim quotes
12
citations
2
research areas
—
Not open access

Abstract

An apparatus was developed for applying heat treatments to spikes on plants growing in the field. This apparatus gave very satisfactory results on self-fertilized zygotes of Marquis wheat. Treatments at 42, 43, and 44 °C. for 20 min. induced chromosome doubling in 2% of the plants.A wide variety of temperature treatments was applied to more than 13,000 wheat florets 16 to 27 hr. after being pollinated with A. glaucum, in an attempt to produce fertile and stable F 1 hybrids. Chromosome doubling was induced in one F 1 plant of Kharkov × A. glaucum by exposure to alternating temperatures of 36° and 109° F. The resulting 84-chromosome plant grew slowly and failed to produce any spikes, as was the case with a number of 42-chromosome plants of this cross. Consequently there is uncertainty as to whether this plant is inherently abnormal aside from chromosome doubling, or whether the chromosome number is too high for normal development. It must be concluded from the results as a whole, that chromosome doubling by means of temperature treatments can be induced only with great difficulty in Triticum–A. glaucum hybrids.

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Claims 6

Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

The heat-treatment apparatus gave very satisfactory results on self-fertilized zygotes of Marquis wheat.

Material
Marquise1
Outcome
very satisfactory resultse1
Setting
in the fielde0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“An apparatus was developed for applying heat treatments to spikes on plants growing in the field”

✓ located verbatim in the source · abstract, characters 0–96 · show in text ↑
e1

“This apparatus gave very satisfactory results on self-fertilized zygotes of Marquis wheat”

✓ located verbatim in the source · abstract, characters 98–187 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Heat treatments at 42, 43 and 44 °C for 20 min induced chromosome doubling in 2% of wheat plants.

Treatment
Treatments at 42, 43, and 44 °C. for 20 min.e2
Outcome
chromosome doublinge2
Value
2%e2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e1

“This apparatus gave very satisfactory results on self-fertilized zygotes of Marquis wheat”

✓ located verbatim in the source · abstract, characters 98–187 · show in text ↑
e2

“Treatments at 42, 43, and 44 °C. for 20 min. induced chromosome doubling in 2% of the plants”

✓ located verbatim in the source · abstract, characters 189–281 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Alternating temperatures of 36 and 109 °F induced chromosome doubling in one F1 plant of Kharkov x A. glaucum.

Material
Kharkov × A. glaucume3
Treatment
alternating temperatures of 36° and 109° Fe3
Outcome
chromosome doublinge3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e1

“This apparatus gave very satisfactory results on self-fertilized zygotes of Marquis wheat”

✓ located verbatim in the source · abstract, characters 98–187 · show in text ↑
e3

“Chromosome doubling was induced in one F 1 plant of Kharkov × A. glaucum by exposure to alternating temperatures of 36° and 109° F”

✓ located verbatim in the source · abstract, characters 479–609 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

The resulting 84-chromosome wheat hybrid plant grew slowly and failed to produce any spikes.

Material
84-chromosome plante4
Outcome
failed to produce any spikese4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e1

“This apparatus gave very satisfactory results on self-fertilized zygotes of Marquis wheat”

✓ located verbatim in the source · abstract, characters 98–187 · show in text ↑
e4

“The resulting 84-chromosome plant grew slowly and failed to produce any spikes, as was the case with a number of 42-chromosome plants of this cross”

✓ located verbatim in the source · abstract, characters 611–758 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

A number of 42-chromosome plants of the same cross also failed to produce spikes.

Material
42-chromosome plants of this crosse4
Outcome
failed to produce any spikese4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e1

“This apparatus gave very satisfactory results on self-fertilized zygotes of Marquis wheat”

✓ located verbatim in the source · abstract, characters 98–187 · show in text ↑
e4

“The resulting 84-chromosome plant grew slowly and failed to produce any spikes, as was the case with a number of 42-chromosome plants of this cross”

✓ located verbatim in the source · abstract, characters 611–758 · show in text ↑
Observationauthor interpretationoriginal resultnegativemeasured

Claim · model-written summary

Chromosome doubling by temperature treatments can be induced in Triticum x A. glaucum hybrids only with great difficulty.

Taxon
TriticumTriticume5
Outcome
chromosome doubling by means of temperature treatmentse5
Qualifier
only with great difficultye5

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum
Triticum · Taxon · picture from its Wikimedia Commons categoryMottaW · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e5

“chromosome doubling by means of temperature treatments can be induced only with great difficulty in Triticum–A. glaucum hybrids”

✓ located verbatim in the source · abstract, characters 998–1125 · show in text ↑