Paper · 2004 · The Journal of Horticultural Science and Biotechnology

Identification of pollen donors for the avocado cultivar Gwen in a mixed orchard by isozyme analysis

Zulkefly Sulaiman; Graham Collins; Jennifer Witherspoon; et al.

5
claims
10
verbatim quotes
9
citations
2
research areas
—
Not open access

Abstract

SummaryIsozyme analysis of developing embryos of the avocado cultivar Gwen was conducted to determine the most likely pollen donors of trees in a mixed orchard including ‘Hass’, ‘Ryan’, ‘Reed’, ‘Bacon’, ‘Fuerte’, and ‘Ettinger’. A total of 130 embryos was collected from 12 ‘Gwen’ trees located adjacent to a range of different potential pollen donor cultivars. As some of the cultivars had identical zymograms at some loci, it was not always possible to identify positively a single male parent for a particular embryo. The most important pollen donor was ‘Ryan’ with a definite contribution of 53% and a possible contribution of 86%. Self-fertilization could not be excluded, with a possible frequency of about 5%, and a maximum of 17% pollination was possible with cultivars of flowering type A, the same as ‘Gwen’. Isozyme patterns for 9% of the embryos could not be accounted for by any of the cultivars in the orchard, and was attributed to pollen from nearby orchards. These results show that ‘Gwen’ is predominantly outcrossed by cultivars of the complementary flowering type, and that the major pollinator is ‘Ryan’.

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Claims 5

Observationobservedoriginal resultmeasured

Claim · model-written summary

Ryan was the most important pollen donor of Gwen, with a definite contribution of 53% and a possible contribution of 86%.

Taxon
avocadoPersea americanae1
Material
Gwene1
Organ / stage
developing embryose1
Interactor
Ryane2pollinator
Outcome
contributione2
Value
53%e2
Qualifier
possible contribution of 86%e2
Setting
mixed orcharde1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Persea americana
Persea americana · TaxonB.navez · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e1

“Isozyme analysis of developing embryos of the avocado cultivar Gwen was conducted to determine the most likely pollen donors of trees in a mixed orchard”

✓ located verbatim in the source · abstract, characters 7–159 · show in text ↑
e2

“The most important pollen donor was ‘Ryan’ with a definite contribution of 53% and a possible contribution of 86%”

✓ located verbatim in the source · abstract, characters 521–634 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Self-fertilization of Gwen could not be excluded, with a possible frequency of about 5%.

Taxon
avocadoPersea americanae1
Material
Gwene1
Organ / stage
developing embryose1
Outcome
Self-fertilizatione3
Value
about 5%e3
Qualifier
could not be excludede3
Setting
mixed orcharde1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Persea americana
Persea americana · TaxonB.navez · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e1

“Isozyme analysis of developing embryos of the avocado cultivar Gwen was conducted to determine the most likely pollen donors of trees in a mixed orchard”

✓ located verbatim in the source · abstract, characters 7–159 · show in text ↑
e3

“Self-fertilization could not be excluded, with a possible frequency of about 5%”

✓ located verbatim in the source · abstract, characters 636–715 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Pollination of Gwen by cultivars of flowering type A was possible at a maximum of 17%.

Taxon
avocadoPersea americanae1
Material
cultivars of flowering type Ae4
Outcome
pollinatione4
Value
17%e4
Qualifier
maximume4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Persea americana
Persea americana · TaxonB.navez · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e1

“Isozyme analysis of developing embryos of the avocado cultivar Gwen was conducted to determine the most likely pollen donors of trees in a mixed orchard”

✓ located verbatim in the source · abstract, characters 7–159 · show in text ↑
e4

“a maximum of 17% pollination was possible with cultivars of flowering type A”

✓ located verbatim in the source · abstract, characters 721–797 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Isozyme patterns for 9% of the embryos could not be matched to any orchard cultivar and were attributed to pollen from nearby orchards.

Taxon
avocadoPersea americanae1
Material
Gwene1
Organ / stage
embryose5
Outcome
Isozyme patternse5
Value
9%e5
Qualifier
attributed to pollen from nearby orchardse5

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Persea americana
Persea americana · TaxonB.navez · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e1

“Isozyme analysis of developing embryos of the avocado cultivar Gwen was conducted to determine the most likely pollen donors of trees in a mixed orchard”

✓ located verbatim in the source · abstract, characters 7–159 · show in text ↑
e5

“Isozyme patterns for 9% of the embryos could not be accounted for by any of the cultivars in the orchard, and was attributed to pollen from nearby orchards”

✓ located verbatim in the source · abstract, characters 819–974 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Gwen is predominantly outcrossed by cultivars of the complementary flowering type.

Taxon
avocadoPersea americanae1
Material
Gwene1
Outcome
outcrossede6
Qualifier
predominantlye6

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Persea americana
Persea americana · TaxonB.navez · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e1

“Isozyme analysis of developing embryos of the avocado cultivar Gwen was conducted to determine the most likely pollen donors of trees in a mixed orchard”

✓ located verbatim in the source · abstract, characters 7–159 · show in text ↑
e6

“is predominantly outcrossed by cultivars of the complementary flowering type”

✓ located verbatim in the source · abstract, characters 1007–1083 · show in text ↑