Abstract
This study was undertaken to standardize an efficient and effective protocol for callus induction, subsequent growth and regeneration in three Pakistani rice (Oryza sativa L.) varieties viz., GNY-53, Basmati-370 and JP-5. MS and N6 media were used for callus induction. Overall, MS medium was found better for callus induction as compared to N6 medium. The growth regulator 2,4-D with varying concentrations (1-3mg/l) were tested for their callus induction and subsequent growth. GNY-53 (83%) and JP-5 (96.66%) showed maximum callus induction frequency on MS medium supplemented with 3mg/l 2,4-D while Basmati-370 (99%) showed higher callus induction frequency on the same medium supplemented with 1mg/l 2,4-D. The callus growth frequencies for varieties GNY-53 (60%) and Basmati-370 (94.73%) were best achieved on MS medium supplemented with 3mg/l 2,4D while JP-5 (73.68%) showed maximum callus growth frequency on the same medium with 1mg/l 2,4-D concentration. Mean square value for variety, treatment and their interactions were recorded for callus induction and growth on both MS and N6 media at probability level of p≤0.05. The regeneration efficiency of these varieties were tested alone on MS medium fortified with two different combinations of NAA and BAP (1mg/l NAA: 2mg/l BAP and 1mg/l NAA:4mg/l BAP). The two varieties GNY-53 (70.27%) and JP-5 (41.81%) showed maximum plantlets formation frequency on 1:2mg/l combination of NAA and BAP whereas Basmati-370 (43.33%) showed higher plantlets formation frequency on 1:4mg/l combination of NAA and BAP. Mean square value for variety, treatment and their interactions were also recorded for regeneration efficiency at probability level of p≤0.05 which showed that these entire factors significantly affect plantlets formation frequency.
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Claims 11
Claim · model-written summary
MS medium gave better callus induction than N6.
- Taxon
- rice
Oryza sativae1 - Treatment
- MS mediume0
- Outcome
- callus inductione0
- Direction
- bettere0
- Comparator
- N6 mediume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overall, MS medium was found better for callus induction as compared to N6 medium”
e1“rice (Oryza sativa L.)”
Claim · model-written summary
GNY-53 had maximum callus induction at 83% on MS plus 3mg/l 2,4-D.
- Taxon
- riceOryza sativae1
- Material
- GNY-53e0
- Treatment
- MS medium supplemented with 3mg/l 2,4-De0
- Outcome
- callus induction frequencye0
- Direction
- maximume0
- Value
- 83%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GNY-53 (83%) and JP-5 (96.66%) showed maximum callus induction frequency on MS medium supplemented with 3mg/l 2,4-D”
e1“rice (Oryza sativa L.)”
Claim · model-written summary
JP-5 had maximum callus induction at 96.66% on MS plus 3mg/l 2,4-D.
- Taxon
- riceOryza sativae1
- Material
- JP-5e0
- Treatment
- MS medium supplemented with 3mg/l 2,4-De0
- Outcome
- callus induction frequencye0
- Direction
- maximume0
- Value
- 96.66%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GNY-53 (83%) and JP-5 (96.66%) showed maximum callus induction frequency on MS medium supplemented with 3mg/l 2,4-D”
e1“rice (Oryza sativa L.)”
Claim · model-written summary
Basmati-370 had higher callus induction at 99% on MS plus 1mg/l 2,4-D.
- Taxon
- riceOryza sativae1
- Material
- Basmati-370e0
- Treatment
- 1mg/l 2,4-De0
- Outcome
- callus induction frequencye0
- Direction
- highere0
- Value
- 99%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Basmati-370 (99%) showed higher callus induction frequency on the same medium supplemented with 1mg/l 2,4-D”
e1“rice (Oryza sativa L.)”
Claim · model-written summary
GNY-53 achieved best callus growth frequency of 60% on MS plus 3mg/l 2,4D.
- Taxon
- riceOryza sativae1
- Material
- GNY-53e0
- Treatment
- MS medium supplemented with 3mg/l 2,4De0
- Outcome
- callus growth frequenciese0
- Direction
- beste0
- Value
- 60%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The callus growth frequencies for varieties GNY-53 (60%) and Basmati-370 (94.73%) were best achieved on MS medium supplemented with 3mg/l 2,4D”
e1“rice (Oryza sativa L.)”
Claim · model-written summary
Basmati-370 achieved best callus growth frequency of 94.73% on MS plus 3mg/l 2,4D.
- Taxon
- riceOryza sativae1
- Material
- Basmati-370e0
- Treatment
- MS medium supplemented with 3mg/l 2,4De0
- Outcome
- callus growth frequenciese0
- Direction
- beste0
- Value
- 94.73%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The callus growth frequencies for varieties GNY-53 (60%) and Basmati-370 (94.73%) were best achieved on MS medium supplemented with 3mg/l 2,4D”
e1“rice (Oryza sativa L.)”
Claim · model-written summary
JP-5 achieved maximum callus growth frequency of 73.68% with 1mg/l 2,4-D.
- Taxon
- riceOryza sativae1
- Material
- JP-5e0
- Treatment
- 1mg/l 2,4-D concentratione0
- Outcome
- callus growth frequencye0
- Direction
- maximume0
- Value
- 73.68%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“JP-5 (73.68%) showed maximum callus growth frequency on the same medium with 1mg/l 2,4-D concentration”
e1“rice (Oryza sativa L.)”
Claim · model-written summary
GNY-53 had maximum plantlet formation of 70.27% under 1:2mg/l NAA and BAP.
- Taxon
- riceOryza sativae1
- Material
- GNY-53e0
- Treatment
- 1:2mg/l combination of NAA and BAPe0
- Outcome
- plantlets formation frequencye0
- Direction
- maximume0
- Value
- 70.27%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GNY-53 (70.27%) and JP-5 (41.81%) showed maximum plantlets formation frequency on 1:2mg/l combination of NAA and BAP”
e1“rice (Oryza sativa L.)”
Claim · model-written summary
JP-5 had maximum plantlet formation of 41.81% under 1:2mg/l NAA and BAP.
- Taxon
- riceOryza sativae1
- Material
- JP-5e0
- Treatment
- 1:2mg/l combination of NAA and BAPe0
- Outcome
- plantlets formation frequencye0
- Direction
- maximume0
- Value
- 41.81%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GNY-53 (70.27%) and JP-5 (41.81%) showed maximum plantlets formation frequency on 1:2mg/l combination of NAA and BAP”
e1“rice (Oryza sativa L.)”
Claim · model-written summary
Basmati-370 had higher plantlet formation of 43.33% under 1:4mg/l NAA and BAP.
- Taxon
- riceOryza sativae1
- Material
- Basmati-370e0
- Treatment
- 1:4mg/l combination of NAA and BAPe0
- Outcome
- plantlets formation frequencye0
- Direction
- highere0
- Value
- 43.33%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Basmati-370 (43.33%) showed higher plantlets formation frequency on 1:4mg/l combination of NAA and BAP”
e1“rice (Oryza sativa L.)”
Claim · model-written summary
Variety, treatment and their interaction significantly affected plantlet formation frequency.
- Taxon
- riceOryza sativae2
- Material
- varietye1
- Outcome
- plantlets formation frequencye0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“these entire factors significantly affect plantlets formation frequency”
e1“Mean square value for variety, treatment and their interactions were also recorded for regeneration efficiency at probability level of p≤0.05”
e2“rice (Oryza sativa L.)”
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