Abstract
In this study, the effects of medium components and environmental conditions on callus induction and regeneration potential of two indica rice varieties (IR64 and MTL250) were evaluated. Mature seeds were cultured on four different medium formulations including either Murashige and Skoog (MS) or Chu (N6) for callus induction. The MS medium was found more suitable than N6 medium in terms of callus induction frequency. The highest callus induction rate with good quality was obtained on MS medium containing 2mg/L 2,4D, 112mg/L B5 vitamin, 500mg/L proline, 500mg/L glutamine, 300mg/L casein hydrolysate, 30g/L sucrose and 4g/L phytagel. To investigate the best condition for callus growth, the influences of incubation temperatures and light conditions on callus induction of IR64 and MTL250 varieties were examined. The experimental results clearly showed that incubation temperatures at both 28oC and 32oC had no significant effects on callus induction in both varieties. However, periodic illumination for 16 hours/day and complete darkness proved the best effect on callus induction in MTL250 and IR64, respectively. Among 07 different media used for shoot induction, the best results were obtained when calli of IR64 and MTL250 were cultured on medium containing MS including vitamins + 2.0 mg/L BAP + 0.5 mg/L NAA + 20g/L sucrose + 30g/L sorbitol + 6g/L phytagel. Shoot regeneration by using medium supplemented with kinetin, moreover, was very low or even failed in both varieties. This plant regeneration protocol is considered to be significant improved and promises to serve for breeding or genetic engineering of indica rice varieties in the Mekong Delta of Viet Nam.
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Claims 8
Claim · model-written summary
MS medium gave higher callus induction frequency than N6 medium.
- Taxon
- indica rice varieties (Oryza sativa L.)
Oryza sativa Indica Groupt0 - Treatment
- MS mediume0
- Outcome
- callus induction frequencye0
- Direction
- more suitablee0
- Comparator
- N6 mediume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The MS medium was found more suitable than N6 medium in terms of callus induction frequency.”
t0“indica rice varieties (Oryza sativa L.)”
Claim · model-written summary
The specified MS medium composition gave the highest rate of good quality callus induction.
- Taxon
- indica rice varieties (Oryza sativa L.)Oryza sativa Indica Groupt0
- Treatment
- MS medium containing 2mg/L 2,4D, 112mg/L B5 vitamin, 500mg/L proline, 500mg/L glutamine, 300mg/L casein hydrolysate, 30g/L sucrose and 4g/L phytagele0
- Outcome
- callus induction rate with good qualitye0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest callus induction rate with good quality was obtained on MS medium containing 2mg/L 2,4D, 112mg/L B5 vitamin, 500mg/L proline, 500mg/L glutamine, 300mg/L casein hydrolysate, 30g/L sucrose and 4g/L phytagel.”
t0“indica rice varieties (Oryza sativa L.)”
Claim · model-written summary
Incubation at 28oC and 32oC did not significantly differ in callus induction in either variety.
- Taxon
- indica rice varieties (Oryza sativa L.)Oryza sativa Indica Groupt0
- Treatment
- incubation temperatures at both 28oC and 32oCe0
- Outcome
- callus inductione0
- Direction
- no significant effectse0
- Comparator
- 28oC and 32oCe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“incubation temperatures at both 28oC and 32oC had no significant effects on callus induction in both varieties.”
t0“indica rice varieties (Oryza sativa L.)”
Claim · model-written summary
Sixteen hours per day of light gave the best callus induction in MTL250.
- Taxon
- indica rice varieties (Oryza sativa L.)Oryza sativa Indica Groupt0
- Material
- MTL250e0
- Treatment
- periodic illumination for 16 hours/daye0
- Outcome
- callus inductione0
- Direction
- beste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“periodic illumination for 16 hours/day and complete darkness proved the best effect on callus induction in MTL250 and IR64, respectively.”
t0“indica rice varieties (Oryza sativa L.)”
Claim · model-written summary
Complete darkness gave the best callus induction in IR64.
- Taxon
- indica rice varieties (Oryza sativa L.)Oryza sativa Indica Groupt0
- Material
- IR64e0
- Treatment
- complete darknesse0
- Outcome
- callus inductione0
- Direction
- beste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“periodic illumination for 16 hours/day and complete darkness proved the best effect on callus induction in MTL250 and IR64, respectively.”
t0“indica rice varieties (Oryza sativa L.)”
Claim · model-written summary
The specified BAP and NAA medium gave the best shoot induction for both varieties.
- Taxon
- indica rice varieties (Oryza sativa L.)Oryza sativa Indica Groupt0
- Treatment
- medium containing MS including vitamins + 2.0 mg/L BAP + 0.5 mg/L NAA + 20g/L sucrose + 30g/L sorbitol + 6g/L phytagele0
- Outcome
- shoot inductione0
- Direction
- beste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Among 07 different media used for shoot induction, the best results were obtained when calli of IR64 and MTL250 were cultured on medium containing MS including vitamins + 2.0 mg/L BAP + 0.5 mg/L NAA + 20g/L sucrose + 30g/L sorbitol + 6g/L phytagel.”
t0“indica rice varieties (Oryza sativa L.)”
Claim · model-written summary
Kinetin supplemented medium produced very low or failed shoot regeneration in both varieties.
- Taxon
- indica rice varieties (Oryza sativa L.)Oryza sativa Indica Groupt0
- Treatment
- medium supplemented with kinetine0
- Outcome
- Shoot regeneratione0
- Direction
- very low or even failede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Shoot regeneration by using medium supplemented with kinetin, moreover, was very low or even failed in both varieties.”
t0“indica rice varieties (Oryza sativa L.)”
Claim · model-written summary
The authors consider the regeneration protocol improved and useful for breeding or genetic engineering.
- Taxon
- indica rice varietiesOryza sativa Indica Groupe0
- Outcome
- plant regeneration protocole0
- Qualifier
- promises to serve for breeding or genetic engineeringe0
- Setting
- Mekong Delta of Viet Name0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This plant regeneration protocol is considered to be significant improved and promises to serve for breeding or genetic engineering of indica rice varieties in the Mekong Delta of Viet Nam.”
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See all 31 papers →8 of 11 groups shown: named compounds and organisms first, then groups whose results differ (checked claim by claim), then those with positive and negative results, then the smaller groups.
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.