Abstract
The objective of the present study was to develop an effective protocol for optimum callus induction and complete plant regeneration for four varieties of rice (Oryza sativa L.) i.e., Super Basmati, Basmati-370, Basmati-371 and Fakhre Malakand. Calli were induced from mature seed scutelum. The Murashige and Skoog (MS) and Chu's N6 media containing hormone 2, 4-D (2, 4-Dichlorophenoxy acetic acid) in different concentrations were used for callus induction. Fakhre Malakand produced maximum calli on N6 media containing 3 mg L(-1) 2,4-D. while other three varieties showed maximum callus induction on N6 media containing 2.5 mg L(-1) 2,4-D. N6 media was found better than MS media for callus induction. For complete plant regeneration the calli of two varieties i.e., Basmati-370 and Basmati-371 were plated on N6 media containing different concentrations of NAA (1-Naphthalene acetic acid) and BAP (6-benzyl aminopurine). The maximum regeneration frequency (%) was observed on N6 media containing NAA 1 mg L(-1) and BAP 2.5 mg L(-1). It took 27-30 days for the callus to regenerate into a complete plant. Basmati-370 produced 4-7 plantlets per callus whereas Basmati-371 produced 4-8 plantlets per callus with regeneration frequencies of 61 and 69%, respectively.
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Claims 9
Claim · model-written summary
Fakhre Malakand produced maximum calli on N6 medium with 3 mg L(-1) 2,4-D.
- Taxon
- rice
Oryza sativae0t0 - Material
- Fakhre Malakande0
- Treatment
- N6 media containing 3 mg L(-1) 2,4-De0
- Outcome
- callie0
- Direction
- maximume0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Callus Induction and in vitro Plant Regeneration of Rice (Oryza sativa L.) Under Various Conditions”
e0“Fakhre Malakand produced maximum calli on N6 media containing 3 mg L(-1) 2,4-D.”
Claim · model-written summary
The other three rice varieties had maximum callus induction on N6 medium with 2.5 mg L(-1) 2,4-D.
- Taxon
- riceOryza sativae0t0
- Material
- other three varietiese0
- Treatment
- N6 media containing 2.5 mg L(-1) 2,4-De0
- Outcome
- callus inductione0
- Direction
- maximume0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Callus Induction and in vitro Plant Regeneration of Rice (Oryza sativa L.) Under Various Conditions”
e0“other three varieties showed maximum callus induction on N6 media containing 2.5 mg L(-1) 2,4-D.”
Claim · model-written summary
N6 medium outperformed MS medium for rice callus induction.
- Taxon
- riceOryza sativae0t0
- Treatment
- N6 mediae0
- Outcome
- callus inductione0
- Direction
- bettere0
- Comparator
- MS mediae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Callus Induction and in vitro Plant Regeneration of Rice (Oryza sativa L.) Under Various Conditions”
e0“N6 media was found better than MS media for callus induction.”
Claim · model-written summary
Regeneration frequency was highest on N6 medium with NAA 1 mg L(-1) and BAP 2.5 mg L(-1).
- Taxon
- riceOryza sativae0t0
- Treatment
- N6 media containing NAA 1 mg L(-1) and BAP 2.5 mg L(-1)e0
- Outcome
- regeneration frequencye0
- Direction
- maximume0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Callus Induction and in vitro Plant Regeneration of Rice (Oryza sativa L.) Under Various Conditions”
e0“The maximum regeneration frequency (%) was observed on N6 media containing NAA 1 mg L(-1) and BAP 2.5 mg L(-1).”
Claim · model-written summary
Rice callus required 27-30 days to regenerate into a complete plant.
- Taxon
- riceOryza sativae0t0
- Outcome
- callus to regenerate into a complete plante0
- Value
- 27-30 dayse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Callus Induction and in vitro Plant Regeneration of Rice (Oryza sativa L.) Under Various Conditions”
e0“It took 27-30 days for the callus to regenerate into a complete plant.”
Claim · model-written summary
Basmati-370 produced 4-7 plantlets per callus.
- Taxon
- riceOryza sativae0t0
- Material
- Basmati-370e0
- Outcome
- plantlets per calluse0
- Value
- 4-7 plantlets per calluse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Callus Induction and in vitro Plant Regeneration of Rice (Oryza sativa L.) Under Various Conditions”
e0“Basmati-370 produced 4-7 plantlets per callus whereas Basmati-371 produced 4-8 plantlets per callus with regeneration frequencies of 61 and 69%, respectively.”
Claim · model-written summary
Basmati-371 produced 4-8 plantlets per callus.
- Taxon
- riceOryza sativae0t0
- Material
- Basmati-371e0
- Outcome
- plantlets per calluse0
- Value
- 4-8 plantlets per calluse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Callus Induction and in vitro Plant Regeneration of Rice (Oryza sativa L.) Under Various Conditions”
e0“Basmati-371 produced 4-8 plantlets per callus with regeneration frequencies of 61 and 69%, respectively.”
Claim · model-written summary
Basmati-370 had a 61% regeneration frequency.
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Callus Induction and in vitro Plant Regeneration of Rice (Oryza sativa L.) Under Various Conditions”
e0“Basmati-370 produced 4-7 plantlets per callus whereas Basmati-371 produced 4-8 plantlets per callus with regeneration frequencies of 61 and 69%, respectively.”
Claim · model-written summary
Basmati-371 had a 69% regeneration frequency.
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Callus Induction and in vitro Plant Regeneration of Rice (Oryza sativa L.) Under Various Conditions”
e0“Basmati-370 produced 4-7 plantlets per callus whereas Basmati-371 produced 4-8 plantlets per callus with regeneration frequencies of 61 and 69%, respectively.”
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