Abstract
Mature embryos of rice (Oryza sativa L.) varieties Jow Haw was cultured on solid MS (Murashige and Skoog, 1962) and NB (Nitch and Nitch, 1969) medium supplemented with 0.5, 1, 2, 3 and 5 mg/l 2,4-dichlorophenoxy acetic acid (2,4-D), 30 g/l sucrose, 1 g/l proline, 100 mg/l casein hydrolysate and 2.6 g/l phytagel. An optimum concentration of 3 mg/l 2,4-D in NB medium was found to be effective for callus induction which induced the biggest size of calli. Transferred the calli to liquid NB medium containing 3 mg/l 2,4-D, 30 g/l sucrose, 1 g/l proline and 100 mg/l casein hydrolysate. Study the growth phases at 0, 3, 6, 9, 12, 15, 18 and 21 days respectively, were determined by measuring fresh and dry weight of the cell. And viability of cell suspensions were determined by the method of fluorescein diacetate. The living cell of cell suspensions showed green fluorescence.
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Claims 2
Claim · model-written summary
NB medium with 3 mg/l 2,4-D produced the biggest rice calli.
- Taxon
- rice
Oryza sativae1 - Treatment
- 3 mg/l 2,4-D in NB mediume0
- Outcome
- size of callie0
- Direction
- biggeste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An optimum concentration of 3 mg/l 2,4-D in NB medium was found to be effective for callus induction which induced the biggest size of calli”
e1“rice (Oryza sativa L.)”
Claim · model-written summary
Living rice suspension cells showed green fluorescence under the viability assay.
- Taxon
- riceOryza sativae1
- Outcome
- green fluorescencee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The living cell of cell suspensions showed green fluorescence.”
e1“rice (Oryza sativa L.)”
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