Paper · 1999 · Shokubutsu Kojo Gakkaishi

Effect of Sugar Concentration on Bulbil Formation In vitro and on Growth after Acclimatization in Narcissus tazetta L. var. chinensis ROEM.

Teruyuki NAGAI

9
claims
18
verbatim quotes
0
citations
3
research areas
✓
Open access

Abstract

Mass regenerated shoots of tissue-cultured shoot primordia from shoot apices in Narcissus tazetta L. var. chinensis ROEM. were cultured on the agar medium containing 0. 2 mg/l NAA and 0.2mg/l BAP at 25°C under continuous lighting condition. At an 8% sugar concentration, shoots formed bulbils and the survival rate was higher. At a 2% sugar concentration, better leaf elongation was observed.Scale segments obtained from shoots that are propagated on medium containing a 3% sugar concentration with 2mg/l NAA and 4 mg/l BAP were cultured before acclimatization on agar medium containing 0.2mg/l NAA and 0.2mg/l BAP. The total fresh weight after culture was greatest at 3% sugar concentration and weight tended to decrease as sugar concentration increased. Total leaf number, shoot number with leaf and length of longest leaf were better at sugar concentration of 3% and 4%. After low temperature treatment, the masses of shoots were planted in pots and acclimatized. Masses cultured at the 8%-12% sugar concentration showed a high survival rate and the total weight of bulb was greater.It was concluded that 18 weeks culture on 8% sugar containing medium was suitable for the growth after acclimatization.

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Claims 9

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

At 8% sugar, shoots formed bulbils and had higher survival.

Taxon
Narcissus tazetta L. var. chinensis ROEM.Narcissus tazetta subsp. chinensist0
Organ / stage
shootse0
Treatment
8% sugar concentratione0
Outcome
survival ratee0
Direction
highere0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Narcissus tazetta subsp. chinensis
Narcissus tazetta subsp. chinensis · TaxonKENPEI · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“At an 8% sugar concentration, shoots formed bulbils and the survival rate was higher”

✓ located verbatim in the source · abstract, characters 241–325 · show in text ↑
t0

“Narcissus tazetta L. var. chinensis ROEM.”

✓ located verbatim in the source · title, characters 98–139 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

At 8% sugar, shoots formed bulbils.

Taxon
Narcissus tazetta L. var. chinensis ROEM.Narcissus tazetta subsp. chinensist0
Treatment
8% sugar concentratione0
Outcome
shoots formed bulbilse0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Narcissus tazetta subsp. chinensis
Narcissus tazetta subsp. chinensis · TaxonKENPEI · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“At an 8% sugar concentration, shoots formed bulbils”

✓ located verbatim in the source · abstract, characters 241–292 · show in text ↑
t0

“Narcissus tazetta L. var. chinensis ROEM.”

✓ located verbatim in the source · title, characters 98–139 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

A 2% sugar concentration gave better leaf elongation.

Taxon
Narcissus tazetta L. var. chinensis ROEM.Narcissus tazetta subsp. chinensist0
Treatment
2% sugar concentratione0
Outcome
leaf elongatione0
Direction
bettere0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Narcissus tazetta subsp. chinensis
Narcissus tazetta subsp. chinensis · TaxonKENPEI · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“At a 2% sugar concentration, better leaf elongation was observed”

✓ located verbatim in the source · abstract, characters 327–391 · show in text ↑
t0

“Narcissus tazetta L. var. chinensis ROEM.”

✓ located verbatim in the source · title, characters 98–139 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Total fresh weight after culture was greatest at 3% sugar.

Taxon
Narcissus tazetta L. var. chinensis ROEM.Narcissus tazetta subsp. chinensist0
Treatment
3% sugar concentratione0
Outcome
total fresh weight after culturee0
Direction
greateste0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Narcissus tazetta subsp. chinensis
Narcissus tazetta subsp. chinensis · TaxonKENPEI · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The total fresh weight after culture was greatest at 3% sugar concentration”

✓ located verbatim in the source · abstract, characters 616–691 · show in text ↑
t0

“Narcissus tazetta L. var. chinensis ROEM.”

✓ located verbatim in the source · title, characters 98–139 · show in text ↑
Associationobservedoriginal resultnegativemeasured

Claim · model-written summary

Fresh weight tended to decrease as sugar concentration increased.

Taxon
Narcissus tazetta L. var. chinensis ROEM.Narcissus tazetta subsp. chinensist0
Treatment
sugar concentratione0
Outcome
weighte0
Direction
decreasee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Narcissus tazetta subsp. chinensis
Narcissus tazetta subsp. chinensis · TaxonKENPEI · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“weight tended to decrease as sugar concentration increased”

✓ located verbatim in the source · abstract, characters 696–754 · show in text ↑
t0

“Narcissus tazetta L. var. chinensis ROEM.”

✓ located verbatim in the source · title, characters 98–139 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

At 3% and 4% sugar, leaf number, leafy shoot number and longest leaf length were better.

Taxon
Narcissus tazetta L. var. chinensis ROEM.Narcissus tazetta subsp. chinensist0
Treatment
sugar concentration of 3% and 4%e0
Outcome
Total leaf number, shoot number with leaf and length of longest leafe0
Direction
bettere0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Narcissus tazetta subsp. chinensis
Narcissus tazetta subsp. chinensis · TaxonKENPEI · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Total leaf number, shoot number with leaf and length of longest leaf were better at sugar concentration of 3% and 4%”

✓ located verbatim in the source · abstract, characters 756–872 · show in text ↑
t0

“Narcissus tazetta L. var. chinensis ROEM.”

✓ located verbatim in the source · title, characters 98–139 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Shoots cultured at 8%–12% sugar had high survival after acclimatization.

Taxon
Narcissus tazetta L. var. chinensis ROEM.Narcissus tazetta subsp. chinensist0
Treatment
8%-12% sugar concentratione0
Outcome
survival ratee0
Direction
highe0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Narcissus tazetta subsp. chinensis
Narcissus tazetta subsp. chinensis · TaxonKENPEI · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Masses cultured at the 8%-12% sugar concentration showed a high survival rate”

✓ located verbatim in the source · abstract, characters 967–1044 · show in text ↑
t0

“Narcissus tazetta L. var. chinensis ROEM.”

✓ located verbatim in the source · title, characters 98–139 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Shoots cultured at 8%–12% sugar had greater total bulb weight.

Taxon
Narcissus tazetta L. var. chinensis ROEM.Narcissus tazetta subsp. chinensist0
Treatment
8%-12% sugar concentratione0
Outcome
total weight of bulbe0
Direction
greatere0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Narcissus tazetta subsp. chinensis
Narcissus tazetta subsp. chinensis · TaxonKENPEI · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Masses cultured at the 8%-12% sugar concentration showed a high survival rate and the total weight of bulb was greater”

✓ located verbatim in the source · abstract, characters 967–1085 · show in text ↑
t0

“Narcissus tazetta L. var. chinensis ROEM.”

✓ located verbatim in the source · title, characters 98–139 · show in text ↑
Recommendationauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

The authors concluded that 18 weeks on 8% sugar medium suited growth after acclimatization.

Taxon
Narcissus tazetta L. var. chinensis ROEM.Narcissus tazetta subsp. chinensist0
Outcome
18 weeks culture on 8% sugar containing mediume0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Narcissus tazetta subsp. chinensis
Narcissus tazetta subsp. chinensis · TaxonKENPEI · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“18 weeks culture on 8% sugar containing medium was suitable for the growth after acclimatization”

✓ located verbatim in the source · abstract, characters 1108–1204 · show in text ↑
t0

“Narcissus tazetta L. var. chinensis ROEM.”

✓ located verbatim in the source · title, characters 98–139 · show in text ↑