Abstract
The tuber of Scirpus yagara Ohwi. (Cyperaceae) has long been used in traditional Chinese medicine (TCM). Several chemical constituents isolated from it possess a variety of physiologically activities such as anti-inflammatory, antitumor and antioxidant. A simultaneous high-performance liquid chromatography (HPLC) analysis was developed and validated for the determination of nine active components in tubers and aerial parts of S. yagara. The analysis was performed on a YMC-Pack ODS-A column (4.6 × 250 mm, 5 μm, 30 nm) with a multilinear gradient mobile phase of water-formic acid (100 : 0.2, v/v) and methanol. The established HPLC method was validated in terms of linearity, sensitivity, precision, accuracy, recovery and stability. All analyzed components were detected in the whole tested samples, and the contents of most components in the aerial parts were even higher than those in the tubers. Moreover, the best harvest period was discovered to be November, which is different from the traditional. The method developed was successfully applied for simultaneous qualitative and quantitative analysis of nine active components in S. yagara.
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Claims 5
Claim · model-written summary
The HPLC method was validated for linearity, sensitivity, precision, accuracy, recovery and stability.
- Taxon
- Scirpus yagara Ohwi.
Bolboschoenus yagarae2 - Outcome
- linearity, sensitivity, precision, accuracy, recovery and stabilitye0
- Value
- nine active componentse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The established HPLC method was validated in terms of linearity, sensitivity, precision, accuracy, recovery and stability”
e1“determination of nine active components”
e2“Scirpus yagara Ohwi.”
Claim · model-written summary
All analyzed components were detected in all tested samples.
- Taxon
- Scirpus yagara Ohwi.Bolboschoenus yagarae1
- Outcome
- All analyzed components were detectede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“All analyzed components were detected in the whole tested samples”
e1“Scirpus yagara Ohwi.”
Claim · model-written summary
Most component contents were higher in aerial parts than tubers.
- Taxon
- Scirpus yagara Ohwi.Bolboschoenus yagarae1
- Organ / stage
- aerial partse0
- Outcome
- contents of most componentse0
- Direction
- highere0
- Comparator
- tuberse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the contents of most components in the aerial parts were even higher than those in the tubers”
e1“Scirpus yagara Ohwi.”
Claim · model-written summary
November was identified as the best harvest period.
- Taxon
- Scirpus yagara Ohwi.Bolboschoenus yagarae1
- Outcome
- best harvest periode0
- Value
- Novembere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the best harvest period was discovered to be November, which is different from the traditional”
e1“Scirpus yagara Ohwi.”
Claim · model-written summary
The method was successfully applied for simultaneous qualitative and quantitative analysis of nine components.
- Taxon
- Scirpus yagara Ohwi.Bolboschoenus yagarae1
- Outcome
- simultaneous qualitative and quantitative analysise0
- Value
- nine active componentse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The method developed was successfully applied for simultaneous qualitative and quantitative analysis of nine active components in S. yagara”
e1“Scirpus yagara Ohwi.”