Abstract
It was previously shown that a crude extract of Agapanthus africanus L. (Hoffman), applied as a foliar spray to wheat (Triticum aestivum L.) seedlings, significantly increased the in vitro activities of three pathogenesis related (PR) proteins (β-1,3-glucanase, chitinase and peroxidase). This was the case in both susceptible and resistant wheat lines whether the plants were uninfected or infected with leaf rust (Puccinia triticina). The aim of this study was to determine the influence of the A. africanus extract on both the intercellular PR-protein profile and PR gene expression in leaf rust infected wheat lines. Pre-treatment of infected resistant and susceptible wheat with the extract led to increased β-1,3-glucanase levels that were higher as compared to the untreated controls. Similarly, treatment with the extract led to greater expression of both the PR3 and PR9 genes in infected resistant and susceptible seedlings as compared to the controls. This is also applied to a retrotransposon protein encoding gene whose expression was strongly induced following extract treatment. The induced expression of all these defence-related genes suggests that the crude A. africanus extract has the ability to prime the resistance response of wheat prior to leaf rust infection. Key words: Wheat leaf rust, induced resistance, priming, gene expression, immunoblotting, crude Agapanthus africanus extract.
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Claims 4
Claim · model-written summary
Pre-treatment with the extract increased β-1,3-glucanase levels in leaf-rust-infected wheat compared with untreated controls.
- Taxon
- wheat
Triticum aestivume0 - Treatment
- the extracte2
- Interactor
- Puccinia triticina
Puccinia triticinae1pathogen - Outcome
- β-1,3-glucanase levelse2
- Direction
- increasede2
- Comparator
- untreated controlse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“applied as a foliar spray to wheat (Triticum aestivum L.) seedlings”
e1“whether the plants were uninfected or infected with leaf rust (Puccinia triticina)”
e2“Pre-treatment of infected resistant and susceptible wheat with the extract led to increased β-1,3-glucanase levels that were higher as compared to the untreated controls”
Claim · model-written summary
The extract increased expression of the PR3 and PR9 genes in leaf-rust-infected wheat compared with controls.
- Taxon
- wheatTriticum aestivume0
- Treatment
- the extracte3
- Interactor
- Puccinia triticinaPuccinia triticinae1pathogen
- Gene
- PR3 and PR9e3
- Outcome
- expressione3
- Direction
- greatere3
- Comparator
- the controlse3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“applied as a foliar spray to wheat (Triticum aestivum L.) seedlings”
e1“whether the plants were uninfected or infected with leaf rust (Puccinia triticina)”
e3“Similarly, treatment with the extract led to greater expression of both the PR3 and PR9 genes in infected resistant and susceptible seedlings as compared to the controls”
Claim · model-written summary
Extract treatment strongly induced expression of a retrotransposon protein encoding gene in wheat seedlings.
- Taxon
- wheatTriticum aestivume0
- Treatment
- extract treatmente4
- Gene
- retrotransposon protein encoding genee4
- Outcome
- expressione4
- Direction
- strongly inducede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“applied as a foliar spray to wheat (Triticum aestivum L.) seedlings”
e4“This is also applied to a retrotransposon protein encoding gene whose expression was strongly induced following extract treatment”
Claim · model-written summary
The crude extract may prime the resistance response of wheat before leaf rust infection.
- Taxon
- wheatTriticum aestivume5
- Treatment
- crude A. africanus extracte5
- Outcome
- resistance responsee5
- Qualifier
- defence-related genese5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“applied as a foliar spray to wheat (Triticum aestivum L.) seedlings”
e5“The induced expression of all these defence-related genes suggests that the crude A. africanus extract has the ability to prime the resistance response of wheat prior to leaf rust infection”
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See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.