Abstract
Kiwifruit (Actinidia deliciosa) is grown for its excellent food and nutritional value in many parts of the world. The main objective of this study was to optimize an efficient, reliable and economical protocol for In vitro micropropagation of Kiwifruit. The effect of coconut water along with BAP on shoot proliferation of Kiwifruit (Actinidia deliciosa) has been evaluated. It has been noticed that both the BAP and coconut water had a synergistic effect and none of them was found able to generate the maximum response when used separately. Maximum shoot length (7.2 ± 0.16), number of shoots (11.5 ± 1.5) and number of nodes (4.6 ± 0.22) were achieved on the MS medium containing 20% (v/v) coconut water with 2.0 mg/L of BAP (KW10). The use of coconut water also resulted in the longer sub-culturing time and the production of highly robust plants which were able to survive in the green house conditions. The proliferated shoots were subjected to root induction and half strength MS media with 0.2 mg/L IBA was found optimum for the root formation. The sufficiently rooted plantlets were transferred to green house for hardening. The potting mix comprised of 90% sand and 10% farm yard manure (v/v) and more than 95% of the plants, subjected to acclimatization, survived under green house conditions.
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Claims 7
Claim · model-written summary
Coconut water and BAP acted synergistically for shoot proliferation.
- Taxon
- kiwifruit
Actinidia deliciosat0 - Treatment
- BAP and coconut watere0
- Outcome
- maximum responsee0
- Direction
- synergistic effecte0
- Comparator
- used separatelye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“both the BAP and coconut water had a synergistic effect and none of them was found able to generate the maximum response when used separately”
t0“kiwifruit (Actinidia deliciosa)”
Claim · model-written summary
The specified medium gave maximum shoot length of 7.2 ± 0.16.
- Taxon
- kiwifruitActinidia deliciosat0
- Treatment
- MS medium containing 20% (v/v) coconut water with 2.0 mg/L of BAP (KW10)e0
- Outcome
- shoot lengthe0
- Direction
- Maximume0
- Value
- 7.2 ± 0.16e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Maximum shoot length (7.2 ± 0.16), number of shoots (11.5 ± 1.5) and number of nodes (4.6 ± 0.22) were achieved on the MS medium containing 20% (v/v) coconut water with 2.0 mg/L of BAP (KW10)”
t0“kiwifruit (Actinidia deliciosa)”
Claim · model-written summary
The same medium gave maximum shoot number of 11.5 ± 1.5.
- Taxon
- kiwifruitActinidia deliciosat0
- Treatment
- MS medium containing 20% (v/v) coconut water with 2.0 mg/L of BAP (KW10)e0
- Outcome
- number of shootse0
- Direction
- Maximume0
- Value
- 11.5 ± 1.5e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Maximum shoot length (7.2 ± 0.16), number of shoots (11.5 ± 1.5) and number of nodes (4.6 ± 0.22) were achieved on the MS medium containing 20% (v/v) coconut water with 2.0 mg/L of BAP (KW10)”
t0“kiwifruit (Actinidia deliciosa)”
Claim · model-written summary
The same medium gave maximum node number of 4.6 ± 0.22.
- Taxon
- kiwifruitActinidia deliciosat0
- Treatment
- MS medium containing 20% (v/v) coconut water with 2.0 mg/L of BAP (KW10)e0
- Outcome
- number of nodese0
- Direction
- Maximume0
- Value
- 4.6 ± 0.22e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Maximum shoot length (7.2 ± 0.16), number of shoots (11.5 ± 1.5) and number of nodes (4.6 ± 0.22) were achieved on the MS medium containing 20% (v/v) coconut water with 2.0 mg/L of BAP (KW10)”
t0“kiwifruit (Actinidia deliciosa)”
Claim · model-written summary
Coconut water lengthened subculturing time and yielded robust greenhouse-surviving plants.
- Taxon
- kiwifruitActinidia deliciosat0
- Treatment
- coconut watere0
- Outcome
- longer sub-culturing time and the production of highly robust plantse0
- Direction
- longere0
- Setting
- green house conditionse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The use of coconut water also resulted in the longer sub-culturing time and the production of highly robust plants which were able to survive in the green house conditions.”
t0“kiwifruit (Actinidia deliciosa)”
Claim · model-written summary
Half-strength MS with IBA was optimal for root formation.
- Taxon
- kiwifruitActinidia deliciosat0
- Treatment
- half strength MS media with 0.2 mg/L IBAe0
- Outcome
- root formatione0
- Direction
- optimume0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“half strength MS media with 0.2 mg/L IBA was found optimum for the root formation”
t0“kiwifruit (Actinidia deliciosa)”
Claim · model-written summary
More than 95% of acclimatized plants survived in the specified potting mix.
- Taxon
- kiwifruitActinidia deliciosat0
- Treatment
- 90% sand and 10% farm yard manure (v/v)e0
- Outcome
- plants, subjected to acclimatization, survivede0
- Direction
- more thane0
- Value
- more than 95%e0
- Setting
- green house conditionse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The potting mix comprised of 90% sand and 10% farm yard manure (v/v) and more than 95% of the plants, subjected to acclimatization, survived under green house conditions.”
t0“kiwifruit (Actinidia deliciosa)”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.